Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KATP_RS03755 Genome accession   NZ_AP022665
Coordinates   783125..784105 (+) Length   326 a.a.
NCBI ID   WP_035892027.1    Uniprot ID   -
Organism   Kluyvera ascorbata strain TP1631     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 778125..789105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KATP_RS03720 (KATP_07350) - 778561..778887 (+) 327 WP_035892034.1 YggL family protein -
  KATP_RS03725 (KATP_07360) - 778940..779659 (+) 720 WP_035892033.1 DUF2884 domain-containing protein -
  KATP_RS03730 (KATP_07370) hemW 779801..780937 (-) 1137 WP_035892032.1 radical SAM family heme chaperone HemW -
  KATP_RS03735 (KATP_07380) - 780930..781523 (-) 594 WP_035892031.1 XTP/dITP diphosphatase -
  KATP_RS03740 (KATP_07390) yggU 781532..781822 (-) 291 WP_035892030.1 DUF167 family protein YggU -
  KATP_RS03745 (KATP_07400) - 781819..782385 (-) 567 WP_035892029.1 YggT family protein -
  KATP_RS03750 (KATP_07410) - 782404..783108 (-) 705 WP_035892028.1 YggS family pyridoxal phosphate-dependent enzyme -
  KATP_RS03755 (KATP_07420) pilT 783125..784105 (+) 981 WP_035892027.1 type IV pilus twitching motility protein PilT Machinery gene
  KATP_RS03760 (KATP_07430) - 784629..785282 (+) 654 WP_035892026.1 LuxR C-terminal-related transcriptional regulator -
  KATP_RS03765 (KATP_07440) ruvX 785384..785803 (-) 420 WP_035892025.1 Holliday junction resolvase RuvX -
  KATP_RS03770 (KATP_07450) - 785803..786366 (-) 564 WP_035892024.1 YqgE/AlgH family protein -
  KATP_RS03775 (KATP_07460) gshB 786512..787459 (-) 948 WP_035892023.1 glutathione synthase -
  KATP_RS03780 (KATP_07470) rsmE 787472..788203 (-) 732 WP_035892022.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KATP_RS03785 (KATP_07480) endA 788282..788989 (-) 708 WP_035892021.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35594.76 Da        Isoelectric Point: 6.7760

>NTDB_id=79104 KATP_RS03755 WP_035892027.1 783125..784105(+) (pilT) [Kluyvera ascorbata strain TP1631]
MDIQEMVSLSVKHNVSDLHLCSSGMARWRQAGQLLIAPFPSPEPQILLDAWLSETQRREWQQRQQLDFALSLTGCPRLRA
SAFAHSGGVSLVLRLLSTHCPRLDELHTPVALAELLREESGLILVTGSTGSGKSTTLAAMVDHLNHQLAGHIVTLEDPVE
FLHESGSCLIHQREVGLHTSSFSEALRAALRQDPDVILLGELRDSDTIRLALTAAETGHLVLATLHTRGAAQAIERLIDV
FPAQEKDPVRNQLASSLCAVLSQKLQSDKHGGRVALFEMLINTPAVGNLIREGKTHQLAGVMQTGLQSGMQTFSQSYQQR
VKAGML

Nucleotide


Download         Length: 981 bp        

>NTDB_id=79104 KATP_RS03755 WP_035892027.1 783125..784105(+) (pilT) [Kluyvera ascorbata strain TP1631]
ATGGATATACAAGAAATGGTGTCGCTTAGTGTAAAGCATAACGTATCGGATCTACACCTATGTAGCAGCGGGATGGCACG
CTGGCGCCAGGCCGGTCAGCTGTTAATTGCGCCATTTCCGTCGCCTGAGCCGCAAATACTGCTGGATGCATGGTTAAGTG
AAACACAGCGGCGCGAGTGGCAGCAGCGCCAACAGCTTGATTTTGCGCTCTCCCTTACCGGTTGCCCGCGCCTGAGGGCC
AGCGCTTTTGCTCATAGCGGCGGGGTTTCATTGGTACTGCGGCTGTTATCTACACATTGCCCAAGGCTCGATGAACTGCA
CACGCCGGTAGCACTCGCGGAGTTACTGCGAGAAGAGAGCGGGCTGATACTGGTGACGGGGTCCACCGGTAGCGGCAAAT
CAACCACGCTCGCCGCGATGGTTGATCACCTCAATCATCAGCTTGCCGGGCATATTGTGACGCTTGAAGACCCCGTCGAA
TTTCTACATGAAAGCGGAAGCTGTTTGATACATCAGCGGGAAGTCGGCCTGCACACCTCCTCATTTTCCGAGGCGCTACG
GGCAGCGCTACGTCAGGACCCCGATGTGATTCTACTGGGTGAGCTACGTGACAGTGACACCATTCGGCTTGCGCTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACGCTGCACACGCGAGGGGCTGCTCAGGCTATCGAACGGTTGATTGACGTG
TTTCCCGCGCAGGAAAAAGATCCGGTGCGTAATCAACTGGCGAGCAGTTTATGCGCGGTGCTATCCCAAAAGCTGCAGTC
GGATAAACACGGAGGTCGCGTCGCGCTGTTTGAAATGCTGATTAACACGCCGGCCGTCGGCAACCTGATTCGTGAAGGTA
AGACGCATCAGCTTGCTGGCGTGATGCAAACCGGGTTACAGAGTGGGATGCAGACTTTTAGCCAGAGCTATCAACAAAGA
GTAAAAGCCGGGATGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.632

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.632

100

0.491
  pilT Neisseria meningitidis 8013

46.97

100

0.475
  pilT Acinetobacter baylyi ADP1

46.809

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.667

100

0.472
  pilT Pseudomonas aeruginosa PAK

45.946

100

0.469
  pilT Acinetobacter baumannii strain A118

46.201

100

0.466
  pilT Acinetobacter baumannii D1279779

46.201

100

0.466
  pilT Acinetobacter nosocomialis M2

46.201

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Legionella pneumophila strain Lp02

45.455

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.455

100

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.304

96.933

0.429
  pilU Pseudomonas stutzeri DSM 10701

41.25

98.16

0.405
  pilU Acinetobacter baylyi ADP1

39.385

99.693

0.393
  pilU Vibrio cholerae strain A1552

39.198

99.387

0.39

Multiple sequence alignment