Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PUP72_RS27050 Genome accession   NZ_CP118134
Coordinates   5949364..5950398 (-) Length   344 a.a.
NCBI ID   WP_056846963.1    Uniprot ID   -
Organism   Pseudomonas synxantha strain ATCC 17413     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5944364..5955398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP72_RS27020 (PUP72_27020) ruvX 5944476..5944913 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  PUP72_RS27025 (PUP72_27025) pyrR 5945047..5945553 (+) 507 WP_046069929.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PUP72_RS27030 (PUP72_27030) - 5945578..5946582 (+) 1005 WP_046069930.1 aspartate carbamoyltransferase catalytic subunit -
  PUP72_RS27035 (PUP72_27035) - 5946579..5947850 (+) 1272 WP_274356769.1 dihydroorotase -
  PUP72_RS27040 (PUP72_27040) - 5947970..5948398 (-) 429 WP_056846962.1 TM2 domain-containing protein -
  PUP72_RS27045 (PUP72_27045) - 5948648..5949286 (+) 639 WP_046069933.1 C40 family peptidase -
  PUP72_RS27050 (PUP72_27050) pilT 5949364..5950398 (-) 1035 WP_056846963.1 type IV pilus twitching motility protein PilT Machinery gene
  PUP72_RS27055 (PUP72_27055) - 5950456..5951142 (+) 687 WP_046069935.1 YggS family pyridoxal phosphate-dependent enzyme -
  PUP72_RS27060 (PUP72_27060) proC 5951177..5951995 (+) 819 WP_046069936.1 pyrroline-5-carboxylate reductase -
  PUP72_RS27065 (PUP72_27065) - 5952021..5952608 (+) 588 WP_046069937.1 YggT family protein -
  PUP72_RS27070 (PUP72_27070) metX 5952750..5953892 (+) 1143 WP_046069938.1 homoserine O-succinyltransferase MetX -
  PUP72_RS27075 (PUP72_27075) metW 5953918..5954538 (+) 621 WP_046069939.1 methionine biosynthesis protein MetW -
  PUP72_RS27080 (PUP72_27080) - 5954560..5954997 (+) 438 WP_046069940.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37625.51 Da        Isoelectric Point: 7.3204

>NTDB_id=790276 PUP72_RS27050 WP_056846963.1 5949364..5950398(-) (pilT) [Pseudomonas synxantha strain ATCC 17413]
MDITQLLTASVSRGASDLHLSAGLVPMLRIDGEVWPMDWPVLEASQMVDLVCPLLNKHQQKDFETSLETDFAFELPGVAR
FRANVFRQDRGVGAVFRTIPTEVQSLEDLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRRQHILTLED
PIEFIHRPKKSLITQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
IDVFPAGEKVMVRSMLSESLQAVVSQVLVKKVGGGRVAAHELMLGTPAIRNLIREDKVAQVVSAIQTGGALGMKTLDMSL
KALVARGVVSRDDAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=790276 PUP72_RS27050 WP_056846963.1 5949364..5950398(-) (pilT) [Pseudomonas synxantha strain ATCC 17413]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGAGGCGCCTCCGACCTGCATCTGTCGGCCGGCCTGGTGCCGAT
GCTGCGCATCGATGGCGAGGTCTGGCCCATGGATTGGCCGGTGCTTGAGGCATCGCAAATGGTCGACCTGGTGTGCCCGT
TGCTGAATAAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGTTGCCCGGGGTGGCGCGT
TTTCGAGCCAACGTGTTCCGCCAGGACCGCGGCGTGGGTGCGGTATTTCGCACTATCCCGACCGAGGTCCAGAGCCTGGA
AGACCTGGGGCTGGGCGAGGTTTTCCAGCGTATCGCCCAGCTTCCACGCGGCCTGGTATTGGTCACTGGGCCCACCGGCT
CGGGTAAGTCCACCACCTTGGCCGCGATGATCGACCACCTTAACCAGCATCGACGCCAGCATATCCTCACCCTGGAAGAT
CCTATTGAATTTATCCACAGGCCGAAAAAGTCCCTGATCACCCAGCGCCAAGTGCACCGCGATACCCACAGCTTTTCGGT
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGATGTGATACTGGTGGGTGAGCTACGCGACCTGGAAACCATTCGCCTGG
CGCTGACGGCCGCTGAGACCGGGCACCTGGTATTTGGCACCCTCCACACTTCATCGGCAGCAAAGACCGTGGACAGGCTG
ATAGACGTCTTCCCTGCCGGGGAAAAGGTCATGGTTCGCTCAATGCTGTCGGAGTCGTTGCAGGCGGTGGTGTCCCAAGT
GCTGGTGAAGAAGGTCGGCGGCGGGCGCGTGGCGGCTCATGAACTCATGCTGGGCACGCCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTGGCGCAGGTGGTCTCGGCGATTCAGACCGGTGGGGCGTTGGGGATGAAGACGCTGGATATGAGTTTG
AAGGCGTTGGTTGCGCGAGGCGTGGTCAGTCGAGACGATGCGCGAGAGAAGGCGAGGGTGCCTGCAGACATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.221

100

0.712
  pilT Pseudomonas stutzeri DSM 10701

70.349

100

0.703
  pilT Acinetobacter baumannii D1279779

70.088

99.128

0.695
  pilT Acinetobacter baumannii strain A118

70.088

99.128

0.695
  pilT Acinetobacter nosocomialis M2

69.795

99.128

0.692
  pilT Acinetobacter baylyi ADP1

68.328

99.128

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.579

97.965

0.642
  pilT Vibrio cholerae strain A1552

65.579

97.965

0.642
  pilT Legionella pneumophila strain Lp02

63.314

98.256

0.622
  pilT Legionella pneumophila strain ERS1305867

63.314

98.256

0.622
  pilT Neisseria meningitidis 8013

60.882

98.837

0.602
  pilT Neisseria gonorrhoeae MS11

60.588

98.837

0.599
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.503
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392
  pilU Vibrio cholerae strain A1552

39.13

100

0.392