Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PQO03_RS07000 Genome accession   NZ_CP117811
Coordinates   1775973..1777028 (+) Length   351 a.a.
NCBI ID   WP_274149042.1    Uniprot ID   -
Organism   Lentisphaera profundi strain SAORIC-696     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1770973..1782028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQO03_RS06975 (PQO03_06975) - 1771569..1772546 (+) 978 WP_274149031.1 L,D-transpeptidase -
  PQO03_RS06980 (PQO03_06980) - 1772552..1773514 (+) 963 WP_274149033.1 acetyl-CoA carboxylase carboxyltransferase subunit alpha -
  PQO03_RS06985 (PQO03_06985) - 1773607..1774194 (+) 588 WP_274149035.1 thioredoxin family protein -
  PQO03_RS06990 (PQO03_06990) - 1774197..1774928 (+) 732 WP_274149037.1 protein-disulfide reductase DsbD domain-containing protein -
  PQO03_RS06995 (PQO03_06995) thiD 1774962..1775780 (-) 819 WP_274149039.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  PQO03_RS07000 (PQO03_07000) pilT 1775973..1777028 (+) 1056 WP_274149042.1 type IV pilus twitching motility protein PilT Machinery gene
  PQO03_RS07005 (PQO03_07005) - 1777038..1778282 (+) 1245 WP_274149044.1 type II secretion system F family protein -
  PQO03_RS07010 (PQO03_07010) - 1778316..1781345 (+) 3030 WP_274149046.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38372.88 Da        Isoelectric Point: 4.9955

>NTDB_id=787623 PQO03_RS07000 WP_274149042.1 1775973..1777028(+) (pilT) [Lentisphaera profundi strain SAORIC-696]
MIEMHDLLQLVIDEGASDLHITVGVAPMLRINGSLNALETEALAAEDTERLMKSITSDKNRVGVSEHGGADFGFAFGEQA
RFRVSVFKQKNTYAMVLRTISNTLLTLEQIGLPPAIKDLLYTPRGLILVTGPTGSGKSTTLASMINVINHERDCHILTVE
DPIEFYHPHRTSIVNQREIGPDVPSFSEALKRALRQDPDVILVGEMRDLETVSAAVTAAETGHLVFGTLHTTGAAETIDR
IIDVFPTDQQEQVRAQLASGLVAVISQALLPKVGGGRVAAFEILISTDSIKSLIRDNKTFQIVSEIQTGQKYGMNTLDAH
LLQLYEEEKISYADMIVKCQDVKAINSNLNL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=787623 PQO03_RS07000 WP_274149042.1 1775973..1777028(+) (pilT) [Lentisphaera profundi strain SAORIC-696]
ATGATTGAAATGCACGACTTACTGCAACTTGTCATTGACGAAGGCGCAAGTGACCTCCACATAACTGTGGGTGTAGCACC
AATGCTACGAATTAACGGCTCTCTAAATGCACTAGAAACTGAAGCACTTGCTGCTGAAGATACAGAGCGGCTAATGAAGT
CAATTACTTCAGATAAAAATCGCGTAGGCGTCAGTGAACACGGCGGTGCCGATTTTGGTTTTGCTTTTGGTGAACAAGCC
CGTTTTCGTGTCTCCGTTTTTAAGCAAAAAAACACTTACGCAATGGTTTTACGTACTATTTCCAATACCCTTCTAACTCT
TGAACAAATCGGGCTACCGCCTGCCATCAAAGACTTACTCTACACCCCTCGTGGACTCATCCTCGTAACTGGCCCAACTG
GCTCGGGAAAATCCACGACTCTCGCATCAATGATAAACGTGATTAATCACGAACGCGACTGCCATATCCTCACAGTAGAA
GATCCTATCGAATTTTATCACCCCCACAGAACTTCAATCGTAAATCAACGCGAAATCGGCCCTGATGTCCCTTCTTTCTC
CGAAGCGCTTAAAAGAGCACTTCGCCAAGACCCTGACGTTATTCTTGTAGGCGAAATGCGCGACCTTGAAACTGTATCAG
CCGCGGTTACCGCTGCTGAAACGGGTCACTTAGTTTTTGGTACACTGCATACTACCGGCGCCGCTGAAACTATAGATAGA
ATTATTGATGTTTTCCCAACAGACCAACAAGAACAAGTCAGAGCTCAATTAGCCTCAGGACTCGTCGCCGTGATTTCACA
AGCACTTTTACCTAAAGTCGGCGGTGGCCGTGTTGCTGCTTTTGAAATTCTAATCTCTACTGACTCTATCAAGTCCCTCA
TTCGTGACAACAAAACCTTCCAAATTGTTTCTGAAATTCAAACAGGACAAAAATACGGCATGAATACTTTAGATGCTCAC
CTACTTCAGCTCTACGAAGAAGAAAAAATTTCTTACGCCGACATGATTGTAAAATGTCAAGACGTCAAAGCTATCAACAG
CAATCTCAACCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.061

97.721

0.519
  pilT Acinetobacter nosocomialis M2

53.333

94.017

0.501
  pilT Acinetobacter baumannii D1279779

53.333

94.017

0.501
  pilT Acinetobacter baumannii strain A118

53.333

94.017

0.501
  pilT Acinetobacter baylyi ADP1

53.251

92.023

0.49
  pilT Pseudomonas stutzeri DSM 10701

51.212

94.017

0.481
  pilT Legionella pneumophila strain ERS1305867

50.909

94.017

0.479
  pilT Legionella pneumophila strain Lp02

50.909

94.017

0.479
  pilT Pseudomonas aeruginosa PAK

50.606

94.017

0.476
  pilT Neisseria gonorrhoeae MS11

47.977

98.575

0.473
  pilT Neisseria meningitidis 8013

50.153

93.162

0.467
  pilT Vibrio cholerae strain A1552

50.155

92.023

0.462
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.155

92.023

0.462
  pilU Acinetobacter baylyi ADP1

43.027

96.011

0.413
  pilU Pseudomonas stutzeri DSM 10701

41.543

96.011

0.399
  pilU Vibrio cholerae strain A1552

41.369

95.726

0.396