Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PS034_RS04020 Genome accession   NZ_CP117659
Coordinates   825725..826705 (+) Length   326 a.a.
NCBI ID   WP_072251619.1    Uniprot ID   -
Organism   Escherichia albertii strain BIA_13     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 820725..831705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PS034_RS03990 (PS034_03990) - 821330..822337 (+) 1008 WP_025238393.1 DUF1202 family protein -
  PS034_RS03995 (PS034_03995) hemW 822402..823538 (-) 1137 WP_059270889.1 radical SAM family heme chaperone HemW -
  PS034_RS04000 (PS034_04000) - 823531..824124 (-) 594 WP_001174776.1 XTP/dITP diphosphatase -
  PS034_RS04005 (PS034_04005) yggU 824132..824422 (-) 291 WP_001277225.1 DUF167 family protein YggU -
  PS034_RS04010 (PS034_04010) yggT 824419..824985 (-) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  PS034_RS04015 (PS034_04015) yggS 825003..825707 (-) 705 WP_000997809.1 pyridoxal phosphate homeostasis protein -
  PS034_RS04020 (PS034_04020) pilT 825725..826705 (+) 981 WP_072251619.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PS034_RS04025 (PS034_04025) ruvX 826889..827305 (-) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  PS034_RS04030 (PS034_04030) - 827305..827868 (-) 564 WP_059216737.1 YqgE/AlgH family protein -
  PS034_RS04035 (PS034_04035) gshB 827977..828927 (-) 951 WP_000593300.1 glutathione synthase -
  PS034_RS04040 (PS034_04040) rsmE 828940..829671 (-) 732 WP_002461297.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PS034_RS04045 (PS034_04045) endA 829751..830458 (-) 708 WP_002461298.1 deoxyribonuclease I -
  PS034_RS04050 (PS034_04050) - 830553..831050 (-) 498 WP_059258680.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36033.25 Da        Isoelectric Point: 5.7939

>NTDB_id=786827 PS034_RS04020 WP_072251619.1 825725..826705(+) (pilT) [Escherichia albertii strain BIA_13]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDIEVLLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPMVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=786827 PS034_RS04020 WP_072251619.1 825725..826705(+) (pilT) [Escherichia albertii strain BIA_13]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACATTGAAGTGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGACTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGCCCGCAACTCGAACAGCTTGG
TGCGCCGATGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCGGCTCAGGCGGTCGAACGGCTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCGGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

38.253

100

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362