Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PS049_RS09970 Genome accession   NZ_CP117562
Coordinates   2061584..2062564 (+) Length   326 a.a.
NCBI ID   WP_273820259.1    Uniprot ID   A0AAX3MQS4
Organism   Escherichia albertii strain BIA_7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2056584..2067564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PS049_RS09940 (PS049_09940) - 2057189..2058196 (+) 1008 WP_273783048.1 DUF1202 family protein -
  PS049_RS09945 (PS049_09945) hemW 2058261..2059397 (-) 1137 WP_000239934.1 radical SAM family heme chaperone HemW -
  PS049_RS09950 (PS049_09950) - 2059390..2059983 (-) 594 WP_001174776.1 XTP/dITP diphosphatase -
  PS049_RS09955 (PS049_09955) yggU 2059991..2060281 (-) 291 WP_001277225.1 DUF167 family protein YggU -
  PS049_RS09960 (PS049_09960) yggT 2060278..2060844 (-) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  PS049_RS09965 (PS049_09965) yggS 2060862..2061566 (-) 705 WP_000997809.1 pyridoxal phosphate homeostasis protein -
  PS049_RS09970 (PS049_09970) pilT 2061584..2062564 (+) 981 WP_273820259.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PS049_RS09975 (PS049_09975) ruvX 2062748..2063164 (-) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  PS049_RS09980 (PS049_09980) - 2063164..2063727 (-) 564 WP_002461296.1 YqgE/AlgH family protein -
  PS049_RS09985 (PS049_09985) gshB 2063836..2064786 (-) 951 WP_273783052.1 glutathione synthase -
  PS049_RS09990 (PS049_09990) rsmE 2064799..2065530 (-) 732 WP_002461297.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PS049_RS09995 (PS049_09995) endA 2065610..2066317 (-) 708 WP_273820260.1 deoxyribonuclease I -
  PS049_RS10000 (PS049_10000) - 2066412..2066909 (-) 498 WP_002461299.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35987.12 Da        Isoelectric Point: 5.7939

>NTDB_id=786389 PS049_RS09970 WP_273820259.1 2061584..2062564(+) (pilT) [Escherichia albertii strain BIA_7]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDIEVLLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERPVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=786389 PS049_RS09970 WP_273820259.1 2061584..2062564(+) (pilT) [Escherichia albertii strain BIA_7]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACATTGAAGTGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGACTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGCCCGCAACTCGAACAGCTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCGGCTCAGGCGGTCGAACGGCCGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCGGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

38.253

100

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362