Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSH68_RS26185 Genome accession   NZ_CP117464
Coordinates   5724459..5725493 (-) Length   344 a.a.
NCBI ID   WP_034109947.1    Uniprot ID   A0A855QRP0
Organism   Pseudomonas lurida strain FP1465     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5719459..5730493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH68_RS26155 (PSH68_26145) ruvX 5719591..5720028 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  PSH68_RS26160 (PSH68_26150) pyrR 5720101..5720607 (+) 507 WP_069078480.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PSH68_RS26165 (PSH68_26155) - 5720632..5721636 (+) 1005 WP_025857712.1 aspartate carbamoyltransferase catalytic subunit -
  PSH68_RS26170 (PSH68_26160) - 5721633..5722904 (+) 1272 WP_098467514.1 dihydroorotase -
  PSH68_RS26175 (PSH68_26165) - 5722995..5723405 (-) 411 WP_164403484.1 TM2 domain-containing protein -
  PSH68_RS26180 (PSH68_26170) - 5723674..5724312 (+) 639 WP_034109945.1 C40 family peptidase -
  PSH68_RS26185 (PSH68_26175) pilT 5724459..5725493 (-) 1035 WP_034109947.1 type IV pilus twitching motility protein PilT Machinery gene
  PSH68_RS26190 (PSH68_26180) - 5725551..5726237 (+) 687 WP_034109949.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSH68_RS26195 (PSH68_26185) proC 5726271..5727089 (+) 819 WP_069078482.1 pyrroline-5-carboxylate reductase -
  PSH68_RS26200 (PSH68_26190) - 5727099..5727689 (+) 591 WP_034109951.1 YggT family protein -
  PSH68_RS26205 (PSH68_26195) - 5727825..5728964 (+) 1140 WP_034109954.1 homoserine O-acetyltransferase -
  PSH68_RS26210 (PSH68_26200) metW 5728972..5729592 (+) 621 WP_034109956.1 methionine biosynthesis protein MetW -
  PSH68_RS26215 (PSH68_26205) - 5729614..5730048 (+) 435 WP_015886348.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37402.36 Da        Isoelectric Point: 6.8388

>NTDB_id=785815 PSH68_RS26185 WP_034109947.1 5724459..5725493(-) (pilT) [Pseudomonas lurida strain FP1465]
MDITELLTASVRRGASDLHLSAGLVPMLRVDGEVWPLDWPVLSALQVADLLSPLLNQHQQKDFETSLETDFAFELPGVAR
FRANVFQQARGMGAVFRTIPCEVRSLESLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDFLNQHRRQHILTLED
PIEFIHTPKIALINQRQVHRDTHDFSTALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRL
VDVFPAGEKAMVRSMLSETLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGAGVISLEAAREKARVPVDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=785815 PSH68_RS26185 WP_034109947.1 5724459..5725493(-) (pilT) [Pseudomonas lurida strain FP1465]
ATGGATATCACAGAATTACTGACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTGTCGGCGGGCCTGGTGCCGAT
GCTGCGGGTGGATGGCGAGGTGTGGCCTTTGGATTGGCCGGTGCTTTCAGCGCTGCAAGTGGCGGACCTATTGAGCCCGC
TGCTCAATCAGCACCAGCAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGTTGCCCGGCGTTGCGCGT
TTCCGGGCAAATGTATTCCAGCAGGCGCGTGGCATGGGCGCGGTATTTCGCACCATCCCGTGCGAAGTCCGGAGCCTGGA
AAGCCTTGGCCTTGGCGAAGTGTTCCAGCGTATCGCCCAGCTACCCCGTGGCCTGGTCTTGGTGACCGGTCCTACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCGATGATCGACTTTCTCAATCAGCATCGGCGTCAGCACATCCTGACCCTCGAAGAC
CCCATCGAATTTATCCACACGCCCAAAATCGCCCTGATCAACCAGCGCCAGGTGCACCGCGACACGCATGACTTTTCCAC
AGCGCTGCGTTCCGCGCTCCGGGAAGACCCGGACGTGATCCTGGTGGGTGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCCGAGACCGGGCACCTGGTATTTGGCACCCTGCACACCACGTCGGCGGCAAAGACCGTAGACCGACTG
GTGGACGTGTTCCCGGCCGGAGAAAAGGCCATGGTCCGCTCGATGCTGTCCGAGACGCTGCAGGCGGTGGTGTCTCAGGT
TCTGGTGAAGAAGGTCGGCGGCGGGCGCGTGGCGGCCCATGAAATCATGTTGGGCACACCGGCCATTCGCAATCTGATTC
GCGAGGACAAGGTGGCGCAGATGGTTTCGGCGATCCAGACGGGCGGGGCGCTGGGGATGAAGACGCTGGACATGAGCTTG
AAGGCGCTGGTCGGGGCAGGAGTGATCAGCCTGGAAGCGGCGCGGGAGAAGGCGAGGGTGCCTGTGGATATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A855QRP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.607

99.128

0.73
  pilT Pseudomonas stutzeri DSM 10701

71.848

99.128

0.712
  pilT Acinetobacter baumannii D1279779

70.088

99.128

0.695
  pilT Acinetobacter baumannii strain A118

70.088

99.128

0.695
  pilT Acinetobacter nosocomialis M2

70.088

99.128

0.695
  pilT Acinetobacter baylyi ADP1

69.501

99.128

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.392

97.965

0.631
  pilT Vibrio cholerae strain A1552

64.392

97.965

0.631
  pilT Legionella pneumophila strain Lp02

63.609

98.256

0.625
  pilT Legionella pneumophila strain ERS1305867

63.609

98.256

0.625
  pilT Neisseria gonorrhoeae MS11

62.059

98.837

0.613
  pilT Neisseria meningitidis 8013

62.059

98.837

0.613
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

99.419

0.497
  pilU Vibrio cholerae strain A1552

38.841

100

0.39
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387
  pilU Pseudomonas stutzeri DSM 10701

39.104

97.384

0.381