Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSH69_RS27990 Genome accession   NZ_CP117461
Coordinates   6211515..6212549 (-) Length   344 a.a.
NCBI ID   WP_305410396.1    Uniprot ID   -
Organism   Pseudomonas sp. FP1740     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6206515..6217549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH69_RS27960 (PSH69_27950) ruvX 6206696..6207142 (+) 447 WP_019580620.1 Holliday junction resolvase RuvX -
  PSH69_RS27965 (PSH69_27955) pyrR 6207241..6207744 (+) 504 WP_086944407.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PSH69_RS27970 (PSH69_27960) - 6207772..6208776 (+) 1005 WP_007939362.1 aspartate carbamoyltransferase catalytic subunit -
  PSH69_RS27975 (PSH69_27965) - 6208773..6210044 (+) 1272 WP_305410394.1 dihydroorotase -
  PSH69_RS27980 (PSH69_27970) - 6210082..6210513 (-) 432 WP_018928709.1 TM2 domain-containing protein -
  PSH69_RS27985 (PSH69_27975) - 6210764..6211384 (+) 621 WP_305410395.1 C40 family peptidase -
  PSH69_RS27990 (PSH69_27980) pilT 6211515..6212549 (-) 1035 WP_305410396.1 type IV pilus twitching motility protein PilT Machinery gene
  PSH69_RS27995 (PSH69_27985) - 6212607..6213293 (+) 687 WP_105340812.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSH69_RS28000 (PSH69_27990) proC 6213328..6214146 (+) 819 WP_305410397.1 pyrroline-5-carboxylate reductase -
  PSH69_RS28005 (PSH69_27995) - 6214157..6214747 (+) 591 WP_305410398.1 YggT family protein -
  PSH69_RS28010 (PSH69_28000) - 6214752..6215045 (+) 294 WP_018928703.1 DUF167 domain-containing protein -
  PSH69_RS28015 (PSH69_28005) - 6215142..6216281 (+) 1140 WP_019580611.1 homoserine O-acetyltransferase -
  PSH69_RS28020 (PSH69_28010) metW 6216289..6216909 (+) 621 WP_007939351.1 methionine biosynthesis protein MetW -
  PSH69_RS28025 (PSH69_28015) - 6216936..6217370 (+) 435 WP_305410399.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38027.64 Da        Isoelectric Point: 6.8712

>NTDB_id=785740 PSH69_RS27990 WP_305410396.1 6211515..6212549(-) (pilT) [Pseudomonas sp. FP1740]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKEVHDLIYDIMNDLQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGDVFRKITEAPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQALIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KDLVTKGVISREHAREKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=785740 PSH69_RS27990 WP_305410396.1 6211515..6212549(-) (pilT) [Pseudomonas sp. FP1740]
ATGGATATCACTGAGCTGCTGGCTTTCAGCGCCAAACAAGGCGCGTCCGACCTGCACCTGTCTGCCGGCCTGCCACCGAT
GATCCGTGTCGATGGCGATGTGCGACGCATCAACTTGCCGGCCCTGGACCACAAGGAGGTGCACGATCTGATCTACGACA
TCATGAACGACCTCCAACGGGTGGACTTCGAGAAGCATCTTGAAACCGACTTTTCCTTCGAAGTGCCCGGCGTGGCGCGT
TTCCGGGTCAATGCCTTCAACCAGAATCGTGGTGCGGGCGCGGTGTTCCGGACCATTCCGTCGAAGGTCCTGAGCATGGA
CGATCTGGGCATGGGGGACGTGTTTCGCAAGATTACCGAAGCGCCCCGGGGCCTGGTGCTGGTGACCGGCCCCACCGGTT
CCGGCAAGTCCACGACGCTGGCGGCGATGATCGATTACCTGAACAACCATCGCCATCACCACATCCTCACCATCGAAGAC
CCCATCGAGTTTGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGAGAAGTCCATCGCGATACACGCAGCTTCGCCAC
GGCCCTGCGCTCGGCCCTGCGTGAAGACCCGGATGTGATCCTGGTGGGGGAAATGCGGGATCTGGAGACTATTCGGCTGG
CATTGACCGCCGCCGAGACCGGTCATCTGGTATTCGGCACGCTACACACCACCTCGGCGGCAAAAACCATCGACCGGGTG
GTGGACGTGTTTCCGGGCGACGAGAAATCGATGGTGCGCTCGATGCTCTCCGAGTCGCTGCTGGCGGTAGTATCCCAGGC
ATTGATCAAGAAAATCGGCGGCGGGCGGGTTGCCGCCCACGAAATCATGCTCGGCACCTCGGCGATCCGTAACCTGATCC
GTGAAGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGTGGGTCGCTGGGGATGCAGACGCTGGATATGTGTCTG
AAGGATCTCGTGACCAAGGGCGTGATCAGTCGCGAGCATGCGCGGGAGAAGGCGCGGTCGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.209

100

0.872
  pilT Pseudomonas aeruginosa PAK

86.628

100

0.866
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

71.802

100

0.718
  pilT Legionella pneumophila strain ERS1305867

71.802

100

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398