Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSH77_RS28440 Genome accession   NZ_CP117459
Coordinates   6197677..6198711 (-) Length   344 a.a.
NCBI ID   WP_305430218.1    Uniprot ID   -
Organism   Pseudomonas extremorientalis strain FP1754     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6192677..6203711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH77_RS28410 (PSH77_28400) ruvX 6192775..6193212 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  PSH77_RS28415 (PSH77_28405) pyrR 6193286..6193792 (+) 507 WP_058426562.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PSH77_RS28420 (PSH77_28410) - 6193817..6194821 (+) 1005 WP_058426561.1 aspartate carbamoyltransferase catalytic subunit -
  PSH77_RS28425 (PSH77_28415) - 6194818..6196089 (+) 1272 WP_305430217.1 dihydroorotase -
  PSH77_RS28430 (PSH77_28420) - 6196236..6196646 (-) 411 WP_162276412.1 TM2 domain-containing protein -
  PSH77_RS28435 (PSH77_28425) - 6196915..6197550 (+) 636 WP_071490370.1 C40 family peptidase -
  PSH77_RS28440 (PSH77_28430) pilT 6197677..6198711 (-) 1035 WP_305430218.1 type IV pilus twitching motility protein PilT Machinery gene
  PSH77_RS28445 (PSH77_28435) - 6198769..6199455 (+) 687 WP_092241976.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSH77_RS28450 (PSH77_28440) proC 6199489..6200307 (+) 819 WP_071490373.1 pyrroline-5-carboxylate reductase -
  PSH77_RS28455 (PSH77_28445) - 6200317..6200907 (+) 591 WP_025859579.1 YggT family protein -
  PSH77_RS28460 (PSH77_28450) - 6201027..6202166 (+) 1140 WP_071490374.1 homoserine O-acetyltransferase -
  PSH77_RS28465 (PSH77_28455) metW 6202174..6202794 (+) 621 WP_034109956.1 methionine biosynthesis protein MetW -
  PSH77_RS28470 (PSH77_28460) - 6202816..6203253 (+) 438 WP_257102893.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37461.23 Da        Isoelectric Point: 6.8433

>NTDB_id=785681 PSH77_RS28440 WP_305430218.1 6197677..6198711(-) (pilT) [Pseudomonas extremorientalis strain FP1754]
MDITELLTASVRRGASDLHLSAGLAPMLRVDGEVWPLDGRVLSPPQVADLLSPLLNQHQQKDFETSLETDFAFELPGVAR
FRANVFRQERGMGAVFRAVPSEVQSLERLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDFLNQHRRQHILTLED
PIEFIHTPNMALINQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETMRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGEGVISREEAQQKARVPGEI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=785681 PSH77_RS28440 WP_305430218.1 6197677..6198711(-) (pilT) [Pseudomonas extremorientalis strain FP1754]
ATGGATATCACTGAATTACTCACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTGTCCGCCGGACTGGCGCCGAT
GCTGCGGGTGGATGGGGAGGTCTGGCCGCTGGATGGGCGGGTGCTTTCACCCCCGCAAGTGGCCGACTTGTTGAGCCCAT
TGCTCAATCAGCACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAACTGCCCGGTGTGGCGCGG
TTCCGCGCCAATGTGTTCCGGCAGGAGCGGGGCATGGGCGCAGTGTTTCGCGCCGTACCGTCCGAGGTCCAGAGCCTGGA
GCGCCTCGGCCTGGGGGAAGTATTCCAGCGTATCGCCCAGCTGCCGCGCGGCCTGGTGCTGGTGACCGGCCCTACCGGCT
CGGGCAAGTCTACGACTCTGGCGGCGATGATCGACTTTCTCAATCAGCATCGACGCCAGCACATCCTCACCCTTGAAGAC
CCCATCGAGTTTATCCACACGCCGAACATGGCATTGATCAACCAACGCCAGGTGCACCGCGACACCCATAGTTTTTCGGT
TGCCCTGCGCTCGGCGCTCAGGGAAGACCCGGACGTGATCCTGGTGGGTGAACTGCGCGACCTGGAAACCATGCGCCTGG
CCCTGACGGCGGCTGAGACCGGGCACCTGGTATTCGGCACCTTGCATACCTCGTCGGCGGCAAAGACCGTGGATCGGCTG
GTGGATGTGTTTCCGGCGGGGGAAAAGGCCATGGTCCGCTCGATGCTGTCGGAGTCGTTGCAGGCAGTGGTGTCCCAGGT
GCTGGTGAAGAAGATCGGCGGGGGGCGGGTGGCGGCCCATGAAATCATGTTGGGTACGCCGGCTATTCGCAATCTGATCC
GTGAGGACAAGGTGGCGCAGATGGTCTCGGCGATCCAGACGGGTGGGGCGCTGGGGATGAAGACGCTGGATATGAGCTTG
AAGGCGTTGGTCGGGGAGGGGGTGATCAGTCGGGAAGAGGCGCAGCAGAAGGCGAGGGTGCCTGGGGAAATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.434

99.128

0.718
  pilT Pseudomonas stutzeri DSM 10701

70.381

99.128

0.698
  pilT Acinetobacter nosocomialis M2

68.622

99.128

0.68
  pilT Acinetobacter baumannii D1279779

68.622

99.128

0.68
  pilT Acinetobacter baumannii strain A118

68.622

99.128

0.68
  pilT Acinetobacter baylyi ADP1

67.742

99.128

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.392

97.965

0.631
  pilT Vibrio cholerae strain A1552

64.392

97.965

0.631
  pilT Legionella pneumophila strain Lp02

62.722

98.256

0.616
  pilT Legionella pneumophila strain ERS1305867

62.722

98.256

0.616
  pilT Neisseria meningitidis 8013

60.588

98.837

0.599
  pilT Neisseria gonorrhoeae MS11

60.294

98.837

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.406

100

0.485
  pilU Vibrio cholerae strain A1552

39.823

98.547

0.392
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387