Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSH60_RS27170 Genome accession   NZ_CP117456
Coordinates   5904312..5905346 (-) Length   344 a.a.
NCBI ID   WP_045791532.1    Uniprot ID   -
Organism   Pseudomonas simiae strain FP1885     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5899312..5910346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH60_RS27140 (PSH60_27135) ruvX 5899382..5899819 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  PSH60_RS27145 (PSH60_27140) pyrR 5899901..5900407 (+) 507 WP_010207223.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PSH60_RS27150 (PSH60_27145) - 5900432..5901436 (+) 1005 WP_010207224.1 aspartate carbamoyltransferase catalytic subunit -
  PSH60_RS27155 (PSH60_27150) - 5901433..5902704 (+) 1272 WP_021492671.1 dihydroorotase -
  PSH60_RS27160 (PSH60_27155) - 5902854..5903264 (-) 411 WP_164673138.1 TM2 domain-containing protein -
  PSH60_RS27165 (PSH60_27160) - 5903533..5904171 (+) 639 WP_021492670.1 C40 family peptidase -
  PSH60_RS27170 (PSH60_27165) pilT 5904312..5905346 (-) 1035 WP_045791532.1 type IV pilus twitching motility protein PilT Machinery gene
  PSH60_RS27175 (PSH60_27170) - 5905404..5906090 (+) 687 WP_010207232.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSH60_RS27180 (PSH60_27175) proC 5906125..5906943 (+) 819 WP_305375390.1 pyrroline-5-carboxylate reductase -
  PSH60_RS27185 (PSH60_27180) - 5906953..5907543 (+) 591 WP_010207234.1 YggT family protein -
  PSH60_RS27190 (PSH60_27185) - 5907751..5908890 (+) 1140 WP_010207235.1 homoserine O-acetyltransferase -
  PSH60_RS27195 (PSH60_27190) metW 5908898..5909518 (+) 621 WP_010207236.1 methionine biosynthesis protein MetW -
  PSH60_RS27200 (PSH60_27195) - 5909540..5909974 (+) 435 WP_010207238.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37527.30 Da        Isoelectric Point: 6.7318

>NTDB_id=785598 PSH60_RS27170 WP_045791532.1 5904312..5905346(-) (pilT) [Pseudomonas simiae strain FP1885]
MDITELLTASVRRGASDLHLSAGLAPMLRVDGEVWPLEGPVLSPTQVADLLSPLLNQHQQKDFETSLETDFAFELPDVAR
FRANVFRQDRGLSAVFRTIPSEVRSLESLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDFLNQHRRHHILTLED
PIEFIHTPKMALINQRQVHRDTHGFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KVLVGEGLVSREDAREKARVPTDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=785598 PSH60_RS27170 WP_045791532.1 5904312..5905346(-) (pilT) [Pseudomonas simiae strain FP1885]
ATGGATATCACTGAACTACTCACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTGTCCGCCGGCCTGGCGCCGAT
GCTGCGGGTTGATGGCGAGGTCTGGCCGCTGGAGGGGCCGGTGCTTTCACCTACGCAAGTAGCGGACTTATTGAGCCCTT
TGCTCAATCAGCATCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCGTTCGAGTTGCCCGACGTGGCGCGT
TTCCGGGCCAATGTGTTCCGGCAGGATCGCGGGCTGAGCGCGGTGTTTCGTACCATACCGTCCGAAGTCCGGAGCTTGGA
AAGCCTTGGTCTCGGTGAAGTATTCCAGCGTATCGCCCAACTGCCCCGGGGCCTCGTGTTGGTCACCGGCCCGACCGGTT
CAGGCAAGTCCACCACCCTGGCGGCGATGATTGATTTTCTCAATCAGCATCGGCGCCATCACATCCTCACCCTTGAAGAC
CCCATCGAATTTATCCACACGCCGAAAATGGCGTTGATCAACCAACGCCAGGTGCATCGCGACACCCATGGCTTTTCGGT
TGCCCTGCGCTCGGCCCTGAGGGAAGACCCGGACGTGATCCTGGTGGGCGAACTGCGTGACTTGGAGACCATCCGCCTGG
CGCTGACGGCGGCCGAGACCGGGCACCTGGTATTTGGCACCCTGCACACCAGTTCGGCCGCAAAGACCGTAGACCGGCTG
GTGGATGTGTTCCCGGCCGGGGAAAAGGCCATGGTCCGCTCGATGTTGTCGGAGTCGTTGCAAGCGGTGGTGTCCCAGGT
GCTGGTGAAGAAGATCGGCGGCGGGCGGGTGGCGGCTCACGAGATCATGCTGGGCACGCCGGCCATTCGGAATTTGATCC
GCGAGGACAAGGTGGCGCAGATGGTCTCAGCGATCCAGACGGGTGGGGCGTTGGGGATGAAGACGTTGGATATGAGCTTG
AAGGTGCTGGTCGGGGAGGGGCTGGTCAGCCGGGAAGACGCGCGGGAGAAGGCGAGGGTACCGACAGACATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.837

100

0.738
  pilT Pseudomonas stutzeri DSM 10701

71.512

100

0.715
  pilT Acinetobacter baumannii D1279779

69.795

99.128

0.692
  pilT Acinetobacter baumannii strain A118

69.795

99.128

0.692
  pilT Acinetobacter nosocomialis M2

69.795

99.128

0.692
  pilT Acinetobacter baylyi ADP1

69.208

99.128

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.688

97.965

0.634
  pilT Vibrio cholerae strain A1552

64.688

97.965

0.634
  pilT Legionella pneumophila strain Lp02

63.905

98.256

0.628
  pilT Legionella pneumophila strain ERS1305867

63.905

98.256

0.628
  pilT Neisseria meningitidis 8013

62.059

98.837

0.613
  pilT Neisseria gonorrhoeae MS11

61.765

98.837

0.61
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.494
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

38.551

100

0.387
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381