Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSH78_RS01885 Genome accession   NZ_CP117452
Coordinates   387227..388261 (+) Length   344 a.a.
NCBI ID   WP_305498174.1    Uniprot ID   -
Organism   Pseudomonas sp. FP198     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 382227..393261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH78_RS01855 (PSH78_01855) - 382530..382964 (-) 435 WP_305498164.1 DUF4426 domain-containing protein -
  PSH78_RS01860 (PSH78_01860) metW 382991..383611 (-) 621 WP_305498166.1 methionine biosynthesis protein MetW -
  PSH78_RS01865 (PSH78_01865) - 383619..384758 (-) 1140 WP_305498167.1 homoserine O-acetyltransferase -
  PSH78_RS01870 (PSH78_01870) - 385031..385621 (-) 591 WP_305498169.1 YggT family protein -
  PSH78_RS01875 (PSH78_01875) proC 385632..386450 (-) 819 WP_305498170.1 pyrroline-5-carboxylate reductase -
  PSH78_RS01880 (PSH78_01880) - 386479..387171 (-) 693 WP_305498172.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSH78_RS01885 (PSH78_01885) pilT 387227..388261 (+) 1035 WP_305498174.1 type IV pilus twitching motility protein PilT Machinery gene
  PSH78_RS01890 (PSH78_01890) - 388410..389021 (-) 612 WP_305498175.1 C40 family peptidase -
  PSH78_RS01895 (PSH78_01895) - 389271..389699 (+) 429 WP_305498177.1 TM2 domain-containing protein -
  PSH78_RS01900 (PSH78_01900) - 389823..391094 (-) 1272 WP_305498179.1 dihydroorotase -
  PSH78_RS01905 (PSH78_01905) - 391091..392095 (-) 1005 WP_003177495.1 aspartate carbamoyltransferase catalytic subunit -
  PSH78_RS01910 (PSH78_01910) pyrR 392120..392626 (-) 507 WP_305498180.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PSH78_RS01915 (PSH78_01915) ruvX 392709..393146 (-) 438 WP_305498182.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38174.82 Da        Isoelectric Point: 7.0157

>NTDB_id=785468 PSH78_RS01885 WP_305498174.1 387227..388261(+) (pilT) [Pseudomonas sp. FP198]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITEAPRGLVLVTGPTGSGKSTTLAAMIDYLNSHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQGFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKIAQMYSSIQTGGSLGMQTLDMCL
KDLVSKGLISREHARERARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=785468 PSH78_RS01885 WP_305498174.1 387227..388261(+) (pilT) [Pseudomonas sp. FP198]
ATGGATATCACTGAATTGCTGGCGTTCAGCGCCAAACAGGGGGCGTCGGACCTGCACCTGTCCGCCGGGCTGCCGCCGAT
GATCCGCGTGGACGGCGATGTACGGCGAATCAACCTGCCGGCGCTGGACCACAAGCAGGTTCATGAGCTGATCTACGACA
TCATGAACGACCGCCAACGAGTGGATTACGAAAAGTTTCTGGAAACCGATTTTTCCTTCGATGTACCCGGCGTGGCGCGA
TTTCGGGTCAATGCCTTCAACCATAATCGGGGCGCGGGGGCCGTATTCCGGACCATTCCTTCAAAAGTCCTGACCATGGA
AGACCTGGGGATGGGCGAGGTGTTTCGCAAGATCACCGAGGCTCCTCGCGGACTGGTGCTGGTAACCGGGCCGACCGGTT
CAGGCAAGTCCACCACCCTGGCGGCCATGATCGATTACCTGAACAGCCACAAGCACCATCACATCCTCACCATCGAAGAC
CCCATCGAATTCGTGCATGAACCGCGCAAGTGTCTGATCAACCAGCGTGAGGTGCATCGCGATACCCAGGGTTTCTCCAC
GGCCCTGCGCTCGGCCCTGCGGGAGGACCCGGATGTGATCCTAGTGGGGGAAATGCGCGACCTGGAGACCATTCGCCTGG
CGCTGACCGCCGCCGAAACCGGGCACCTGGTGTTCGGCACCCTGCACACCACGTCGGCGGCCAAGACGATTGACCGGGTG
GTGGATGTGTTTCCGGGCGACGAGAAGTCGATGGTCCGCTCGATGCTCTCGGAGTCGCTTCAGGCGGTGATCTCCCAGAC
GCTGGTCAAGAAGATTGGCGGCGGCCGGATCGCGGCGCACGAGATCATGCTGGGCACTTCGGCGATCCGCAACCTGATCC
GCGAAGACAAGATCGCGCAGATGTATTCATCGATCCAGACCGGCGGCTCGCTGGGGATGCAGACGCTGGACATGTGCCTG
AAGGATCTGGTCAGCAAGGGGCTGATCAGCCGCGAGCATGCGCGGGAGCGGGCGCGTACGCCGGATAATTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.372

100

0.884
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.965

0.666
  pilT Vibrio cholerae strain A1552

67.953

97.965

0.666
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392