Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSH76_RS28500 Genome accession   NZ_CP117447
Coordinates   6262667..6263701 (-) Length   344 a.a.
NCBI ID   WP_305493299.1    Uniprot ID   A0AAJ6KSH2
Organism   Pseudomonas sp. FP215     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6257667..6268701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH76_RS28470 (PSH76_28460) ruvX 6257667..6258104 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  PSH76_RS28475 (PSH76_28465) pyrR 6258253..6258759 (+) 507 WP_032894723.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PSH76_RS28480 (PSH76_28470) - 6258784..6259788 (+) 1005 WP_014720325.1 aspartate carbamoyltransferase catalytic subunit -
  PSH76_RS28485 (PSH76_28475) - 6259785..6261056 (+) 1272 WP_305493297.1 dihydroorotase -
  PSH76_RS28490 (PSH76_28480) - 6261202..6261630 (-) 429 WP_032894720.1 TM2 domain-containing protein -
  PSH76_RS28495 (PSH76_28485) - 6261880..6262518 (+) 639 WP_305493298.1 C40 family peptidase -
  PSH76_RS28500 (PSH76_28490) pilT 6262667..6263701 (-) 1035 WP_305493299.1 type IV pilus twitching motility protein PilT Machinery gene
  PSH76_RS28505 (PSH76_28495) - 6263759..6264445 (+) 687 WP_032894716.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSH76_RS28510 (PSH76_28500) proC 6264479..6265297 (+) 819 WP_056783041.1 pyrroline-5-carboxylate reductase -
  PSH76_RS28515 (PSH76_28505) - 6265323..6265910 (+) 588 WP_003195087.1 YggT family protein -
  PSH76_RS28520 (PSH76_28510) - 6266131..6267270 (+) 1140 WP_032894712.1 homoserine O-acetyltransferase -
  PSH76_RS28525 (PSH76_28515) metW 6267278..6267898 (+) 621 WP_003195091.1 methionine biosynthesis protein MetW -
  PSH76_RS28530 (PSH76_28520) - 6267920..6268354 (+) 435 WP_032894709.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37324.11 Da        Isoelectric Point: 7.2995

>NTDB_id=785349 PSH76_RS28500 WP_305493299.1 6262667..6263701(-) (pilT) [Pseudomonas sp. FP215]
MDITQLLAASVSRGASDLHLSAGLAPMLRIDGEVWPLDGPALNATQVVDLLSPLLNKHQQKDFETSLETDFSFELPGVAR
FRANVFRQDRGVGAVFRAIPAEVQSLEDLGLSEVFQRIARLPRGLVLVTGPTGSGKSTTLAAMIDDLNRHRRQHILTLED
PIEFIHRPKMALVNQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAVKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGAGVISREEAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=785349 PSH76_RS28500 WP_305493299.1 6262667..6263701(-) (pilT) [Pseudomonas sp. FP215]
ATGGATATCACGCAATTGCTGGCGGCCAGCGTAAGCCGAGGCGCCTCAGACCTGCATCTGTCAGCCGGCCTGGCACCGAT
GCTGCGCATTGATGGCGAGGTTTGGCCCCTGGATGGGCCGGCGCTGAACGCTACCCAGGTGGTCGACTTATTGAGCCCTT
TGCTGAATAAACATCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTTCCTTCGAATTGCCAGGCGTCGCCCGG
TTCCGGGCGAACGTGTTCCGCCAGGATCGCGGCGTGGGTGCGGTGTTTCGGGCTATCCCGGCTGAGGTACAGAGCCTGGA
GGACCTGGGATTGAGCGAGGTGTTCCAGCGAATCGCCCGCCTCCCGCGCGGCCTAGTGCTGGTGACCGGGCCTACCGGCT
CCGGCAAATCCACGACCCTGGCGGCGATGATCGACGACCTCAACCGGCATCGACGCCAGCATATCCTCACGCTGGAAGAC
CCCATCGAGTTTATCCACAGGCCGAAAATGGCCTTGGTCAACCAGCGCCAGGTGCATCGCGACACCCACAGTTTCTCGGT
GGCCCTGCGTTCAGCCCTGCGCGAAGACCCGGATGTGATCCTGGTGGGTGAACTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCAGAGACCGGGCACCTGGTGTTTGGCACCTTGCACACCTCGTCGGCGGTAAAGACAGTAGACAGGCTG
GTGGACGTCTTCCCGGCCGGGGAGAAGGCGATGGTCCGCTCGATGCTGTCGGAGTCGCTGCAGGCGGTGGTGTCTCAGGT
GCTGGTGAAGAAGGTGGGCGGCGGGCGCGTGGCCGCCCATGAAATCATGCTGGGCACCCCGGCGATTCGTAACTTGATTC
GCGAAGACAAGGTGGCGCAGATGGTTTCGGCTATTCAGACGGGTGGGGCGTTGGGGATGAAGACCCTGGATATGAGTTTG
AAGGCGTTGGTCGGCGCGGGAGTGATTAGCCGGGAAGAGGCGCGGGAGAAGGCGAGGGTGCCGGCAGATATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(40-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.674

100

0.727
  pilT Pseudomonas stutzeri DSM 10701

71.512

100

0.715
  pilT Acinetobacter baumannii D1279779

69.501

99.128

0.689
  pilT Acinetobacter baumannii strain A118

69.501

99.128

0.689
  pilT Acinetobacter nosocomialis M2

69.501

99.128

0.689
  pilT Acinetobacter baylyi ADP1

68.328

99.128

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.985

97.965

0.637
  pilT Vibrio cholerae strain A1552

64.985

97.965

0.637
  pilT Legionella pneumophila strain Lp02

64.497

98.256

0.634
  pilT Legionella pneumophila strain ERS1305867

64.497

98.256

0.634
  pilT Neisseria meningitidis 8013

61.471

98.837

0.608
  pilT Neisseria gonorrhoeae MS11

61.176

98.837

0.605
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.497
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

39.403

97.384

0.384
  pilU Acinetobacter baylyi ADP1

37.572

100

0.378