Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSH79_RS26095 Genome accession   NZ_CP117445
Coordinates   5810256..5811290 (-) Length   344 a.a.
NCBI ID   WP_187679032.1    Uniprot ID   -
Organism   Pseudomonas sp. FP2196     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5805256..5816290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH79_RS26065 (PSH79_26060) ruvX 5805331..5805768 (+) 438 WP_008077844.1 Holliday junction resolvase RuvX -
  PSH79_RS26070 (PSH79_26065) pyrR 5805866..5806372 (+) 507 WP_305440304.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PSH79_RS26075 (PSH79_26070) - 5806399..5807403 (+) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  PSH79_RS26080 (PSH79_26075) - 5807400..5808671 (+) 1272 WP_305440305.1 dihydroorotase -
  PSH79_RS26085 (PSH79_26080) - 5808856..5809290 (-) 435 WP_305440307.1 TM2 domain-containing protein -
  PSH79_RS26090 (PSH79_26085) - 5809539..5810162 (+) 624 WP_305440308.1 C40 family peptidase -
  PSH79_RS26095 (PSH79_26090) pilT 5810256..5811290 (-) 1035 WP_187679032.1 type IV pilus twitching motility protein PilT Machinery gene
  PSH79_RS26100 (PSH79_26095) - 5811347..5812033 (+) 687 WP_305440310.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSH79_RS26105 (PSH79_26100) proC 5812057..5812875 (+) 819 WP_305440311.1 pyrroline-5-carboxylate reductase -
  PSH79_RS26110 (PSH79_26105) - 5812886..5813476 (+) 591 WP_095186992.1 YggT family protein -
  PSH79_RS26115 (PSH79_26110) - 5813473..5813763 (+) 291 WP_305440312.1 DUF167 domain-containing protein -
  PSH79_RS26120 (PSH79_26115) - 5813989..5815128 (+) 1140 WP_305440313.1 homoserine O-acetyltransferase -
  PSH79_RS26125 (PSH79_26120) metW 5815136..5815756 (+) 621 WP_305440314.1 methionine biosynthesis protein MetW -
  PSH79_RS26130 (PSH79_26125) - 5815783..5816217 (+) 435 WP_305440315.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38087.73 Da        Isoelectric Point: 7.0157

>NTDB_id=785302 PSH79_RS26095 WP_187679032.1 5810256..5811290(-) (pilT) [Pseudomonas sp. FP2196]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=785302 PSH79_RS26095 WP_187679032.1 5810256..5811290(-) (pilT) [Pseudomonas sp. FP2196]
ATGGATATCACTGAATTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAATTGATCTACGACA
TCATGAACGACACCCAGCGCGTCGACTTCGAGAAACACCTGGAAACGGATTTCTCCTTCGAAGTCCCTGGTGTCGCGCGC
TTCCGGGTCAATGCCTTCAACCAGAACCGTGGCGCTGGCGCGGTATTCCGCACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGTGATGTCTTCCGCAAGATCACCGACGCCCCGCGTGGGCTGGTATTGGTGACCGGGCCGACCGGTT
CCGGCAAGTCGACCACGCTGGCGGCGATGATCGATTATCTCAACACCCATCGTCATCACCACATCCTGACCATCGAAGAC
CCGATCGAATTCGTTCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCACCGCGATACCCGCAGTTTCGCCAC
GGCGTTGCGTTCAGCGCTGCGCGAAGATCCGGATGTGATTCTGGTGGGCGAGATGCGTGACCTGGAAACCATTCGTCTCG
CACTGACTGCTGCTGAGACCGGGCATCTGGTGTTCGGCACGCTGCACACCACGTCGGCAGCCAAGACCATCGACCGGGTG
GTGGACGTATTCCCCGGTGACGAGAAGTCGATGGTGCGTTCGATGCTGTCAGAGTCGTTGCTGGCGGTGGTGTCGCAGAC
GCTGATCAAGAAGATCGGCGGCGGGCGCGTGGCGGCGCACGAGATCATGCTCGGCACATCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGTCTATTCAGACGGGTGGGTCGTTGGGGATGCAGACACTGGACATGTGCCTG
AAAGATCTGGTGACCAAAGGCTTGATCAGCCGCGAGCATGCGCGGGAGAAGGCGCGTACGCCGGATAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

72.781

98.256

0.715
  pilT Legionella pneumophila strain ERS1305867

72.781

98.256

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398