NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	PSH73_RS24790	Genome accession	NZ_CP117441
Coordinates	5487129..5488163 (-)	Length	344 a.a.
NCBI ID	WP_120734288.1	Uniprot ID	-
Organism	Pseudomonas sp. FP2294
Function	type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5482129..5493163

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSH73_RS24760 (PSH73_24755)	ruvX	5482211..5482648 (+)	438	WP_005792259.1	Holliday junction resolvase RuvX	-
PSH73_RS24765 (PSH73_24760)	pyrR	5482731..5483237 (+)	507	WP_057444186.1	bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR	-
PSH73_RS24770 (PSH73_24765)	-	5483262..5484266 (+)	1005	WP_057440370.1	aspartate carbamoyltransferase catalytic subunit	-
PSH73_RS24775 (PSH73_24770)	-	5484263..5485534 (+)	1272	WP_057440371.1	dihydroorotase	-
PSH73_RS24780 (PSH73_24775)	-	5485697..5486125 (-)	429	WP_057440372.1	TM2 domain-containing protein	-
PSH73_RS24785 (PSH73_24780)	-	5486375..5487010 (+)	636	WP_057440373.1	C40 family peptidase	-
PSH73_RS24790 (PSH73_24785)	pilT	5487129..5488163 (-)	1035	WP_120734288.1	type IV pilus twitching motility protein PilT	Machinery gene
PSH73_RS24795 (PSH73_24790)	-	5488221..5488907 (+)	687	WP_057960885.1	YggS family pyridoxal phosphate-dependent enzyme	-
PSH73_RS24800 (PSH73_24795)	proC	5488941..5489759 (+)	819	WP_271043684.1	pyrroline-5-carboxylate reductase	-
PSH73_RS24805 (PSH73_24800)	-	5489769..5490359 (+)	591	WP_057440376.1	YggT family protein	-
PSH73_RS24810 (PSH73_24805)	-	5490514..5491653 (+)	1140	WP_057440377.1	homoserine O-acetyltransferase	-
PSH73_RS24815 (PSH73_24810)	metW	5491661..5492281 (+)	621	WP_029300485.1	methionine biosynthesis protein MetW	-
PSH73_RS24820 (PSH73_24815)	-	5492303..5492737 (+)	435	WP_029300487.1	DUF4426 domain-containing protein	-

Sequence

Protein

Download Length: 344 a.a. Molecular weight: 37311.94 Da Isoelectric Point: 6.8923

                                >NTDB_id=785198 PSH73_RS24790 WP_120734288.1 5487129..5488163(-) (pilT) [Pseudomonas sp. FP2294]

MDITELLAASVSQGASDLHLSAGLAPRMRVDGEVLPLDWPVLSATQVADLLSPILHKHQQKDFETSLETDFSFDLPGVAR

FRANVFCQHRGLGAVFRAIPSRVQSLEALGLGDIFRRIAELPRGLVLVTGATGSGKSTTLAAMVDYLNSTRQQHILTLED

PIEFVHEPRKALISQRQVHRDTHSFSTALRSALREDPDVILVGELRDLETIRLALTASETGHLVFGTLHTSSAAKTVDRL

VDVFPAGEKAMVRSMLSESLQAVVSQVLLKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQLYSAIQTGGALGMKTLDMSL

KGLVSQGLVRLEEAREQARVPADI

Nucleotide

Download Length: 1035 bp

                                >NTDB_id=785198 PSH73_RS24790 WP_120734288.1 5487129..5488163(-) (pilT) [Pseudomonas sp. FP2294]

ATGGACATCACTGAACTGCTGGCCGCAAGCGTCAGCCAGGGCGCCTCCGACCTGCATCTGTCGGCAGGCTTGGCGCCGAG

GATGCGCGTCGATGGCGAGGTACTGCCACTGGATTGGCCGGTGCTGAGTGCTACGCAAGTAGCGGACTTGCTCAGCCCGA

TCCTCCATAAACACCAGCAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTTCCTTCGATTTGCCTGGCGTCGCGCGC

TTTCGCGCCAACGTGTTCTGCCAGCACCGGGGGCTGGGGGCGGTGTTCCGTGCGATCCCCTCTCGGGTCCAGAGCCTGGA

AGCCCTGGGGCTGGGGGACATATTCCGGCGCATCGCCGAATTGCCCAGAGGGCTGGTGCTGGTGACTGGTGCCACGGGCT

CGGGCAAGTCCACCACCCTGGCGGCGATGGTCGATTACCTCAACAGCACCCGTCAGCAGCACATCCTGACCCTTGAAGAC

CCTATCGAGTTTGTCCACGAACCGCGTAAAGCGCTGATCAGCCAACGCCAGGTGCACCGCGACACCCACAGTTTCTCGAC

GGCCCTGCGTTCGGCGTTGCGCGAAGACCCGGATGTGATCCTGGTTGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG

CCCTGACGGCGTCGGAAACCGGGCACTTGGTGTTTGGCACCCTGCACACCAGTTCGGCGGCGAAAACCGTGGATCGGCTG

GTGGATGTGTTTCCGGCGGGGGAAAAGGCCATGGTCCGCTCGATGCTCTCGGAATCGCTGCAGGCGGTGGTGTCCCAGGT

GTTGCTGAAGAAAGTCGGCGGCGGGCGCGTGGCGGCCCATGAAATCATGCTCGGCACACCGGCGATCCGCAACCTGATCC

GCGAGGACAAGGTGGCGCAGTTGTATTCGGCGATTCAGACGGGCGGGGCGCTGGGGATGAAGACGTTGGATATGAGCTTG

AAGGGGTTGGTCAGTCAGGGACTGGTCAGGCTTGAAGAGGCCCGGGAGCAGGCGCGGGTGCCCGCAGATATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Pseudomonas aeruginosa PAK	72.674	100	0.727
pilT	Pseudomonas stutzeri DSM 10701	71.512	100	0.715
pilT	Acinetobacter nosocomialis M2	70.088	99.128	0.695
pilT	Acinetobacter baumannii D1279779	69.795	99.128	0.692
pilT	Acinetobacter baumannii strain A118	69.795	99.128	0.692
pilT	Acinetobacter baylyi ADP1	69.795	99.128	0.692
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	63.953	100	0.64
pilT	Vibrio cholerae strain A1552	63.953	100	0.64
pilT	Legionella pneumophila strain Lp02	63.609	98.256	0.625
pilT	Legionella pneumophila strain ERS1305867	63.609	98.256	0.625
pilT	Neisseria meningitidis 8013	62.647	98.837	0.619
pilT	Neisseria gonorrhoeae MS11	62.353	98.837	0.616
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	48.986	100	0.491
pilU	Vibrio cholerae strain A1552	38.551	100	0.387
pilU	Pseudomonas stutzeri DSM 10701	39.104	97.384	0.381
pilU	Acinetobacter baylyi ADP1	36.705	100	0.369

Detailed information NTG0785198