Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSH96_RS27500 Genome accession   NZ_CP117436
Coordinates   6053433..6054467 (-) Length   344 a.a.
NCBI ID   WP_305456371.1    Uniprot ID   -
Organism   Pseudomonas sp. FP2338     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6048433..6059467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH96_RS27470 (PSH96_27465) ruvX 6048491..6048928 (+) 438 WP_003213744.1 Holliday junction resolvase RuvX -
  PSH96_RS27475 (PSH96_27470) pyrR 6049011..6049517 (+) 507 WP_056857700.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PSH96_RS27480 (PSH96_27475) - 6049542..6050546 (+) 1005 WP_056857699.1 aspartate carbamoyltransferase catalytic subunit -
  PSH96_RS27485 (PSH96_27480) - 6050543..6051814 (+) 1272 WP_138813466.1 dihydroorotase -
  PSH96_RS27490 (PSH96_27485) - 6051970..6052398 (-) 429 WP_138813465.1 TM2 domain-containing protein -
  PSH96_RS27495 (PSH96_27490) - 6052648..6053286 (+) 639 WP_305456369.1 C40 family peptidase -
  PSH96_RS27500 (PSH96_27495) pilT 6053433..6054467 (-) 1035 WP_305456371.1 type IV pilus twitching motility protein PilT Machinery gene
  PSH96_RS27505 (PSH96_27500) - 6054525..6055211 (+) 687 WP_305456373.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSH96_RS27510 (PSH96_27505) proC 6055245..6056063 (+) 819 WP_305456375.1 pyrroline-5-carboxylate reductase -
  PSH96_RS27515 (PSH96_27510) - 6056089..6056676 (+) 588 WP_305456377.1 YggT family protein -
  PSH96_RS27520 (PSH96_27515) - 6056871..6058010 (+) 1140 WP_305456379.1 homoserine O-acetyltransferase -
  PSH96_RS27525 (PSH96_27520) metW 6058018..6058638 (+) 621 WP_016972957.1 methionine biosynthesis protein MetW -
  PSH96_RS27530 (PSH96_27525) - 6058660..6059097 (+) 438 WP_017139456.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37212.03 Da        Isoelectric Point: 7.0820

>NTDB_id=785073 PSH96_RS27500 WP_305456371.1 6053433..6054467(-) (pilT) [Pseudomonas sp. FP2338]
MDITELLTASVRRGASDLHLSAGLAPMLRVDGEIWPLDGPALSPTQVADLLSPLLNQHQQKDFETSLETDFAFALPGLAR
FRANVFRQDRGMGAVFRTIATEVQSLEDLGLGEVFRRIAQLPCGLVLVTGATGSGKSTTLAAMIDHLNQHRRQHILTLED
PIEFIHRPKMALINQRQVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAAKSVDRM
VDVFPAGEKAMVRAMLSESLQAVVSQVLVKKLGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGEGVISREVARGQARVPTDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=785073 PSH96_RS27500 WP_305456371.1 6053433..6054467(-) (pilT) [Pseudomonas sp. FP2338]
ATGGATATCACTGAACTGCTGACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTGTCGGCCGGCCTGGCGCCGAT
GCTGCGTGTCGATGGCGAAATCTGGCCCTTGGATGGCCCGGCGCTTTCGCCTACGCAAGTTGCGGACTTGTTAAGCCCTT
TGCTCAATCAGCACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGCGCTGCCGGGCCTGGCGCGG
TTCCGCGCGAATGTGTTCCGCCAGGATCGCGGCATGGGCGCGGTGTTTCGCACCATTGCGACCGAAGTGCAGAGCCTGGA
AGACCTCGGATTAGGCGAGGTCTTCCGCCGTATCGCGCAGTTGCCGTGCGGCCTGGTGCTGGTGACCGGGGCGACCGGCT
CCGGCAAGTCGACGACGCTGGCGGCGATGATCGACCACCTCAACCAGCACCGACGCCAGCACATCCTCACGCTGGAAGAC
CCGATCGAATTTATCCACAGGCCGAAAATGGCCCTGATCAACCAGCGCCAGGTACACCGCGATACCCATAGCTTTTCGGC
GGCGCTGCGGTCGGCCCTGCGTGAGGACCCGGATGTGATTCTGGTGGGCGAGCTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACGGCCGCAGAGACCGGGCACTTGGTATTGGGCACGCTGCACACCACCTCAGCGGCAAAGAGCGTGGATCGGATG
GTGGACGTGTTCCCGGCGGGGGAAAAAGCCATGGTGCGCGCCATGTTGTCGGAGTCGTTGCAGGCGGTGGTGTCCCAGGT
GCTGGTCAAGAAGCTCGGCGGCGGCCGGGTGGCGGCGCATGAAATCATGCTGGGTACGCCAGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGGTCTCGGCGATTCAAACCGGCGGGGCGTTGGGGATGAAAACGCTGGATATGAGCTTG
AAGGCGTTGGTCGGCGAGGGGGTGATCAGCCGGGAGGTGGCGCGGGGGCAGGCGCGGGTGCCGACAGATATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

70.349

100

0.703
  pilT Pseudomonas stutzeri DSM 10701

69.186

100

0.692
  pilT Acinetobacter baumannii D1279779

68.915

99.128

0.683
  pilT Acinetobacter baumannii strain A118

68.915

99.128

0.683
  pilT Acinetobacter nosocomialis M2

68.915

99.128

0.683
  pilT Acinetobacter baylyi ADP1

68.328

99.128

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.029

100

0.622
  pilT Vibrio cholerae strain A1552

62.029

100

0.622
  pilT Legionella pneumophila strain Lp02

62.722

98.256

0.616
  pilT Legionella pneumophila strain ERS1305867

62.722

98.256

0.616
  pilT Neisseria meningitidis 8013

60

98.837

0.593
  pilT Neisseria gonorrhoeae MS11

59.412

98.837

0.587
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.45

96.802

0.488
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392