Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JLD64_RS18175 Genome accession   NZ_AP022508
Coordinates   3847400..3848380 (-) Length   326 a.a.
NCBI ID   WP_193031033.1    Uniprot ID   -
Organism   Enterobacter bugandensis strain STN0717-56     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3842400..3853380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JLD64_RS18145 (STN0717ENT56_35030) - 3843289..3843786 (+) 498 WP_028014364.1 SprT family zinc-dependent metalloprotease -
  JLD64_RS18150 (STN0717ENT56_35040) endA 3843881..3844588 (+) 708 WP_028014365.1 deoxyribonuclease I -
  JLD64_RS18155 (STN0717ENT56_35050) rsmE 3844640..3845371 (+) 732 WP_100170841.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JLD64_RS18160 (STN0717ENT56_35060) gshB 3845391..3846338 (+) 948 WP_041908326.1 glutathione synthase -
  JLD64_RS18165 (STN0717ENT56_35070) - 3846413..3846973 (+) 561 WP_028014368.1 YqgE/AlgH family protein -
  JLD64_RS18170 (STN0717ENT56_35080) ruvX 3846973..3847389 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  JLD64_RS18175 (STN0717ENT56_35090) pilT 3847400..3848380 (-) 981 WP_193031033.1 type IV pilus twitching motility protein PilT Machinery gene
  JLD64_RS18180 (STN0717ENT56_35100) - 3848398..3849099 (+) 702 WP_199886719.1 YggS family pyridoxal phosphate-dependent enzyme -
  JLD64_RS18185 (STN0717ENT56_35110) - 3849121..3849687 (+) 567 WP_045260768.1 YggT family protein -
  JLD64_RS18190 (STN0717ENT56_35120) yggU 3849684..3849980 (+) 297 WP_047364545.1 DUF167 family protein YggU -
  JLD64_RS18195 (STN0717ENT56_35130) - 3849984..3850577 (+) 594 WP_193031031.1 XTP/dITP diphosphatase -
  JLD64_RS18200 (STN0717ENT56_35140) hemW 3850570..3851712 (+) 1143 WP_193031029.1 radical SAM family heme chaperone HemW -
  JLD64_RS18205 (STN0717ENT56_35150) - 3851770..3852114 (+) 345 WP_370551781.1 endonuclease domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35597.68 Da        Isoelectric Point: 6.3904

>NTDB_id=78378 JLD64_RS18175 WP_193031033.1 3847400..3848380(-) (pilT) [Enterobacter bugandensis strain STN0717-56]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGDQRLRG
SAFKQMKGISVTLRLLPRSCPQLPSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNRHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLIDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=78378 JLD64_RS18175 WP_193031033.1 3847400..3848380(-) (pilT) [Enterobacter bugandensis strain STN0717-56]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTGGGCCGCCTTGAGCCGGCCCCGTTTCCGCCGCCGGATGTCGGAGCGTTGCTAAAGGCGTGGCTTAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCGCAGGAGACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCATCTCCGTTACCCTGCGGCTGTTGCCGCGTTCCTGTCCGCAGCTCCCTTCGCTTGG
GGTGCCGCGCGCTATTCCCGAACTGTTGTCCAGCGACAACGGGTTGATTCTGGTAACGGGAGCGACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCAATGGTCGACTTCCTCAACCGCCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAGGCGCTGCG
CAGTGCGTTACGCGAGGACCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGGGCAGCACAGGCGATTGAACGGCTGATCGATACT
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCAGTGCTGGCGCAAAAGCTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTACGAGCTGCTGGTAAACACCGCGGCGGCGGCGAATCTGATTCGTGAGGGGA
AGACGTGGCAGCTGCCCGGCATCATTCAAACCGGCCAGCAGGCGGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCACAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Legionella pneumophila strain Lp02

50

98.773

0.494
  pilT Legionella pneumophila strain ERS1305867

50

98.773

0.494
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Acinetobacter baylyi ADP1

48.624

100

0.488
  pilT Acinetobacter baumannii D1279779

48.624

100

0.488
  pilT Acinetobacter baumannii strain A118

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

49.062

98.16

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

98.773

0.42
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment