Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PO881_RS15735 Genome accession   NZ_CP117199
Coordinates   3433327..3434319 (-) Length   330 a.a.
NCBI ID   WP_101761906.1    Uniprot ID   -
Organism   Pantoea sp. SS70     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3428327..3439319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PO881_RS15705 (PO881_15705) - 3428985..3429497 (+) 513 WP_277974072.1 SprT family zinc-dependent metalloprotease -
  PO881_RS15710 (PO881_15710) endA 3429586..3430293 (+) 708 WP_277974073.1 deoxyribonuclease I -
  PO881_RS15715 (PO881_15715) rsmE 3430367..3431098 (+) 732 WP_008107252.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PO881_RS15720 (PO881_15720) gshB 3431176..3432120 (+) 945 WP_101761909.1 glutathione synthase -
  PO881_RS15725 (PO881_15725) - 3432203..3432766 (+) 564 WP_101761908.1 YqgE/AlgH family protein -
  PO881_RS15730 (PO881_15730) ruvX 3432766..3433182 (+) 417 WP_277974074.1 Holliday junction resolvase RuvX -
  PO881_RS15735 (PO881_15735) pilT 3433327..3434319 (-) 993 WP_101761906.1 type IV pilus twitching motility protein PilT Machinery gene
  PO881_RS15740 (PO881_15740) - 3434336..3435043 (+) 708 WP_101761905.1 YggS family pyridoxal phosphate-dependent enzyme -
  PO881_RS15745 (PO881_15745) - 3435056..3435610 (+) 555 WP_008107240.1 YggT family protein -
  PO881_RS15750 (PO881_15750) - 3435631..3436224 (+) 594 WP_110868025.1 XTP/dITP diphosphatase -
  PO881_RS15755 (PO881_15755) hemW 3436217..3437368 (+) 1152 WP_277974075.1 radical SAM family heme chaperone HemW -
  PO881_RS15760 (PO881_15760) - 3437409..3439172 (-) 1764 WP_277974076.1 Ig-like domain-containing protein -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35747.08 Da        Isoelectric Point: 6.6168

>NTDB_id=782821 PO881_RS15735 WP_101761906.1 3433327..3434319(-) (pilT) [Pantoea sp. SS70]
MELDEIVALSVKHNAADLHLCSGHLPHWRRQGVLEPIPQQSALDGGWLEAFMQQWLTASQQAELEENGHVDFAITLASGI
RLRANLFMQRHGLSLALRLIASQAPDLSSLHLPEVVSQLLQLEEGLILITGATGSGKSTTLAAMVDCLNRQQARHILTLE
DPIEFVHRSQRSLIQQREVGAHCASFQHGLKAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGATQAVDR
LVDVFPAEEKNLVRTQLAGSLKAVLAQRLVPAKAGGRIGLFEVLVATPAVANLIREGKMHQLPGVLQTGAQAGMQTFAQS
EQGRQAAGMI

Nucleotide


Download         Length: 993 bp        

>NTDB_id=782821 PO881_RS15735 WP_101761906.1 3433327..3434319(-) (pilT) [Pantoea sp. SS70]
ATGGAGTTGGATGAAATAGTGGCCCTTAGTGTAAAGCATAACGCCGCCGATCTGCACCTTTGCAGCGGACATTTGCCGCA
CTGGCGGCGACAAGGCGTGCTGGAGCCGATTCCGCAGCAGAGCGCGCTGGATGGCGGCTGGCTGGAAGCCTTTATGCAGC
AGTGGCTCACGGCGTCGCAGCAGGCCGAGCTGGAGGAGAACGGCCACGTTGACTTCGCGATTACGCTTGCCAGCGGCATA
CGGCTGCGCGCCAACCTGTTTATGCAGCGTCACGGCTTATCGCTGGCGCTACGGCTGATTGCCAGCCAGGCGCCCGATCT
CAGCAGCCTGCATCTGCCCGAGGTGGTGAGCCAGCTGTTACAGCTGGAAGAGGGACTGATTTTAATTACCGGCGCCACCG
GCAGCGGCAAATCGACCACGCTGGCGGCAATGGTGGATTGTCTGAATCGTCAGCAGGCGCGGCACATTCTGACGCTGGAA
GATCCGATTGAGTTTGTGCATCGCAGCCAGCGCTCGCTGATTCAACAGCGCGAAGTAGGTGCGCACTGCGCCTCGTTTCA
ACACGGGCTGAAAGCCGCGCTGCGTGAAGATCCTGATGTGATTCTGCTCGGCGAACTGCGCGACAGCGAAACCATCCGCC
TGGCGCTCACTGCAGCAGAAACCGGGCATCTGGTGCTGGCTACGCTCCATACGCGCGGCGCAACCCAGGCGGTTGATCGG
CTGGTGGATGTCTTTCCCGCCGAGGAGAAAAACCTGGTGCGCACTCAATTAGCGGGCAGCCTGAAAGCGGTGCTGGCGCA
ACGTTTAGTGCCGGCAAAAGCGGGCGGGCGGATTGGCTTGTTTGAAGTGTTGGTGGCGACGCCAGCGGTGGCAAACCTGA
TCCGCGAAGGCAAGATGCATCAGTTGCCTGGCGTGTTGCAAACCGGCGCGCAGGCTGGCATGCAAACTTTTGCGCAGAGC
GAGCAGGGGCGTCAGGCGGCGGGGATGATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.348

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.348

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.193

100

0.485
  pilT Vibrio cholerae strain A1552

48.193

100

0.485
  pilT Pseudomonas stutzeri DSM 10701

46.97

100

0.47
  pilT Acinetobacter baylyi ADP1

46.667

100

0.467
  pilT Pseudomonas aeruginosa PAK

46.061

100

0.461
  pilT Acinetobacter baumannii D1279779

46.061

100

0.461
  pilT Acinetobacter baumannii strain A118

46.061

100

0.461
  pilT Acinetobacter nosocomialis M2

45.758

100

0.458
  pilT Legionella pneumophila strain Lp02

46.708

96.667

0.452
  pilT Legionella pneumophila strain ERS1305867

46.708

96.667

0.452
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.342

97.576

0.442
  pilU Pseudomonas stutzeri DSM 10701

41.615

97.576

0.406
  pilU Vibrio cholerae strain A1552

41.433

97.273

0.403
  pilU Acinetobacter baylyi ADP1

37.578

97.576

0.367