Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NL414_RS05315 Genome accession   NZ_CP117013
Coordinates   1008732..1009712 (+) Length   326 a.a.
NCBI ID   WP_001604836.1    Uniprot ID   -
Organism   Escherichia coli strain MLI114     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1003732..1014712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL414_RS05285 (NL414_005285) - 1004109..1005095 (-) 987 WP_000784004.1 TRAP transporter substrate-binding protein -
  NL414_RS05290 (NL414_005290) hemW 1005409..1006545 (-) 1137 WP_257843506.1 radical SAM family heme chaperone HemW -
  NL414_RS05295 (NL414_005295) rdgB 1006538..1007131 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  NL414_RS05300 (NL414_005300) yggU 1007139..1007429 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  NL414_RS05305 (NL414_005305) yggT 1007426..1007992 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NL414_RS05310 (NL414_005310) yggS 1008010..1008714 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  NL414_RS05315 (NL414_005315) pilT 1008732..1009712 (+) 981 WP_001604836.1 type IV pilus twitching motility protein PilT Machinery gene
  NL414_RS05320 (NL414_005320) ruvX 1009827..1010243 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  NL414_RS05325 (NL414_005325) yqgE 1010243..1010806 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  NL414_RS05330 (NL414_005330) gshB 1010915..1011865 (-) 951 WP_000593273.1 glutathione synthase -
  NL414_RS05335 (NL414_005335) rsmE 1011878..1012609 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NL414_RS05340 (NL414_005340) endA 1012689..1013396 (-) 708 WP_000286497.1 deoxyribonuclease I -
  NL414_RS05345 (NL414_005345) yggI 1013491..1013988 (-) 498 WP_001296359.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36033.27 Da        Isoelectric Point: 5.7980

>NTDB_id=781991 NL414_RS05315 WP_001604836.1 1008732..1009712(+) (pilT) [Escherichia coli strain MLI114]
MYMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINIPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=781991 NL414_RS05315 WP_001604836.1 1008732..1009712(+) (pilT) [Escherichia coli strain MLI114]
ATGTATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCGAATGGAAGCTGCGCCATTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTGGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACATTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTAGGGGAAGGACGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.405

96.933

0.411
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

38.125

98.16

0.374
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362