Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PFZ56_RS03645 Genome accession   NZ_CP116502
Coordinates   771208..772242 (-) Length   344 a.a.
NCBI ID   WP_170001811.1    Uniprot ID   -
Organism   Pseudomonas danubii strain JDS02PS016     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 766208..777242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFZ56_RS03615 (PFZ56_03595) ruvX 766341..766778 (+) 438 WP_016964942.1 Holliday junction resolvase RuvX -
  PFZ56_RS03620 (PFZ56_03600) pyrR 766895..767401 (+) 507 WP_060841533.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PFZ56_RS03625 (PFZ56_03605) - 767427..768431 (+) 1005 WP_011064007.1 aspartate carbamoyltransferase catalytic subunit -
  PFZ56_RS03630 (PFZ56_03610) - 768428..769699 (+) 1272 WP_170001813.1 dihydroorotase -
  PFZ56_RS03635 (PFZ56_03615) - 769827..770246 (-) 420 WP_102862075.1 TM2 domain-containing protein -
  PFZ56_RS03640 (PFZ56_03620) - 770498..771121 (+) 624 WP_102862076.1 C40 family peptidase -
  PFZ56_RS03645 (PFZ56_03625) pilT 771208..772242 (-) 1035 WP_170001811.1 type IV pilus twitching motility protein PilT Machinery gene
  PFZ56_RS03650 (PFZ56_03630) - 772426..773124 (+) 699 WP_170001809.1 YggS family pyridoxal phosphate-dependent enzyme -
  PFZ56_RS03655 (PFZ56_03635) proC 773159..773977 (+) 819 WP_102862079.1 pyrroline-5-carboxylate reductase -
  PFZ56_RS03660 (PFZ56_03640) - 774002..774589 (+) 588 WP_102862080.1 YggT family protein -
  PFZ56_RS03665 (PFZ56_03645) - 774589..774882 (+) 294 WP_170001807.1 DUF167 domain-containing protein -
  PFZ56_RS03670 (PFZ56_03650) - 775046..776185 (+) 1140 WP_102862082.1 homoserine O-acetyltransferase -
  PFZ56_RS03675 (PFZ56_03655) metW 776193..776813 (+) 621 WP_011064017.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37365.15 Da        Isoelectric Point: 6.8919

>NTDB_id=777639 PFZ56_RS03645 WP_170001811.1 771208..772242(-) (pilT) [Pseudomonas danubii strain JDS02PS016]
MDITELLTLSISRGASDLHLSAGLAPMLRIDGDIRPLDSPALDAQQVLALMHGLMSEPQRQVFASQQDLDFAYELPGVAR
FRVNAFQHSRGAAAVLRLIPSRVLSLEALGLGQVFQQIAEAPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRQQHILTLED
PIEFIHPQKSCLIHQREVPRDTRSFATALRAALREDPDVILLGELRDLESIRLALTAAETGHLVFATLHTASAAKSIDRL
VDVFPGEEKALVRSMLAESLQAVIAQVLLKKVGGGRVAAHEIMLGNAAIRNLIREDKVAQMYSAIQTGGAQGMRTLDMSL
KALLGQGLISREQARDQARMPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=777639 PFZ56_RS03645 WP_170001811.1 771208..772242(-) (pilT) [Pseudomonas danubii strain JDS02PS016]
ATGGACATCACCGAACTCCTGACTTTGAGCATCTCCCGGGGCGCTTCCGACCTGCATCTGTCTGCCGGACTGGCGCCGAT
GTTGCGCATTGATGGCGATATTCGTCCGCTGGACAGCCCGGCGCTGGATGCACAGCAGGTGCTGGCCTTGATGCATGGCC
TGATGAGCGAGCCACAGCGGCAGGTGTTCGCCAGCCAGCAGGATCTGGACTTTGCCTACGAACTGCCGGGCGTGGCGCGG
TTCCGGGTCAATGCCTTCCAGCACTCCCGGGGAGCGGCTGCAGTGCTGCGCCTGATCCCGTCACGGGTACTGAGCTTGGA
GGCGTTGGGACTGGGGCAAGTGTTTCAGCAGATTGCCGAAGCGCCCCGGGGGCTGGTGCTGGTCACCGGGCCCACCGGTA
GCGGCAAGTCCACCACCTTGGCGGCGATGATCGATCACCTGAACCAGCATCGGCAGCAGCACATCCTCACCCTCGAAGAC
CCCATCGAGTTCATTCATCCGCAGAAAAGTTGCCTGATCCACCAGCGCGAAGTGCCGCGTGATACCCGCAGTTTCGCCAC
CGCCTTGCGTGCGGCGCTGCGGGAGGACCCGGACGTGATCCTGCTGGGAGAGTTGCGCGATCTGGAAAGCATTCGCCTGG
CCCTGACCGCCGCCGAAACCGGGCACCTGGTGTTCGCCACCTTGCACACTGCATCCGCGGCCAAGAGCATCGACCGGCTG
GTGGACGTGTTCCCGGGGGAGGAAAAGGCCCTGGTGCGCTCGATGCTGGCCGAGTCGTTGCAGGCGGTCATTGCCCAGGT
GTTGCTGAAGAAAGTGGGCGGTGGGCGGGTGGCGGCTCACGAAATCATGCTGGGCAATGCGGCGATCCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGGGCCCAGGGCATGCGCACCCTGGACATGAGCCTC
AAGGCATTGCTGGGGCAAGGGCTGATCAGCCGAGAACAGGCGCGGGATCAGGCCCGGATGCCGGAGAGCTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

68.895

100

0.689
  pilT Pseudomonas stutzeri DSM 10701

68.895

100

0.689
  pilT Acinetobacter nosocomialis M2

66.57

100

0.666
  pilT Acinetobacter baumannii D1279779

66.57

100

0.666
  pilT Acinetobacter baumannii strain A118

66.57

100

0.666
  pilT Acinetobacter baylyi ADP1

65.988

100

0.66
  pilT Legionella pneumophila strain Lp02

61.834

98.256

0.608
  pilT Legionella pneumophila strain ERS1305867

61.834

98.256

0.608
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

60.882

98.837

0.602
  pilT Vibrio cholerae strain A1552

60.882

98.837

0.602
  pilT Neisseria meningitidis 8013

59.71

100

0.599
  pilT Neisseria gonorrhoeae MS11

59.42

100

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40.597

97.384

0.395
  pilU Acinetobacter baylyi ADP1

38.841

100

0.39
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387