Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   O1Q74_RS03805 Genome accession   NZ_CP116477
Coordinates   787161..788177 (+) Length   338 a.a.
NCBI ID   WP_271876199.1    Uniprot ID   -
Organism   Pectobacterium sp. A5351     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 782161..793177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q74_RS03770 (O1Q74_03765) - 782363..782623 (-) 261 WP_271876193.1 type II toxin-antitoxin system ParD family antitoxin -
  O1Q74_RS03775 (O1Q74_03770) hemW 782850..783992 (-) 1143 WP_271876194.1 radical SAM family heme chaperone HemW -
  O1Q74_RS03780 (O1Q74_03775) - 783985..784578 (-) 594 WP_271876195.1 XTP/dITP diphosphatase -
  O1Q74_RS03785 (O1Q74_03780) yggU 784610..784900 (-) 291 WP_271876196.1 DUF167 family protein YggU -
  O1Q74_RS03790 (O1Q74_03785) - 784897..785451 (-) 555 WP_010295003.1 YggT family protein -
  O1Q74_RS03795 (O1Q74_03790) proC 785500..786321 (-) 822 WP_271876197.1 pyrroline-5-carboxylate reductase -
  O1Q74_RS03800 (O1Q74_03795) - 786412..787140 (-) 729 WP_271876198.1 YggS family pyridoxal phosphate-dependent enzyme -
  O1Q74_RS03805 (O1Q74_03800) pilT 787161..788177 (+) 1017 WP_271876199.1 type IV pilus twitching motility protein PilT Machinery gene
  O1Q74_RS03810 (O1Q74_03805) - 788146..789972 (-) 1827 WP_271876200.1 diguanylate cyclase -
  O1Q74_RS03815 (O1Q74_03810) - 790496..792067 (-) 1572 WP_271876201.1 methyl-accepting chemotaxis protein -
  O1Q74_RS03820 (O1Q74_03815) nsrR 792378..792803 (+) 426 WP_015841587.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37040.29 Da        Isoelectric Point: 6.8228

>NTDB_id=777484 O1Q74_RS03805 WP_271876199.1 787161..788177(+) (pilT) [Pectobacterium sp. A5351]
MELDEWIARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPEQVAQWCDAWLEPAQREQLRQFGQVDGALMLADGQ
RLRVNVFRQRQGLSAALRIIPSAQPSLDGLQAPPILSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGTHSASFAQALRATLREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPATQGGRIALYEVLTVTAAVSNLIREGKTHQLPGLIQTGTAAGMQTFEQS
YQQRCRDRLISHSYDPVV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=777484 O1Q74_RS03805 WP_271876199.1 787161..788177(+) (pilT) [Pectobacterium sp. A5351]
ATGGAATTGGATGAGTGGATAGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCATCTTTGTAGCGGGCATCCGCCAGT
ATTGCGTGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACACCGGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCCGCGCAGCGAGAGCAACTGCGGCAGTTTGGTCAAGTGGACGGTGCGTTAATGCTGGCTGATGGGCAA
CGTCTGCGGGTGAATGTGTTTCGCCAGCGGCAAGGGCTGTCTGCGGCGCTGCGTATTATTCCGTCTGCTCAGCCTTCATT
GGACGGGCTACAGGCACCACCCATTCTATCGACGTTACTGGAAAAACCGAATGGGCTGATTCTGATTACCGGTGCGACGG
GGAGTGGAAAGTCCACGACGCTGGCAGCCATGCTCGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAATTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGCGGGAGATCGGTACGCACAGCGCATCTTTTGC
GCAGGCGTTACGGGCGACATTGCGGGAAGATCCTGATGTGATTCTGTTGGGGGAATTGCGTGATACGGAGACGATCCGCC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTATCCACGCTGCATACGCGCAGTGCCTCTCAGGCGGTCGATCGT
TTGATTGACGTCTTTCCCGGCGAAGAGAAAGCCTATGTTCGCAGCCAGCTCGCCACCTGTTTGCAAGCTGTGGTGACGCA
AAAGTTATTGCCAGCCACACAAGGTGGCCGCATCGCGCTTTATGAAGTACTGACGGTGACAGCGGCAGTGAGCAACCTGA
TTCGGGAGGGGAAAACACACCAGCTTCCCGGCCTGATTCAAACGGGAACGGCTGCGGGAATGCAAACGTTCGAACAAAGC
TACCAGCAGCGCTGTAGGGATAGGCTTATTTCACACAGTTACGACCCTGTCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.393

95.562

0.491
  pilT Vibrio cholerae strain A1552

51.393

95.562

0.491
  pilT Neisseria meningitidis 8013

49.699

98.225

0.488
  pilT Neisseria gonorrhoeae MS11

49.398

98.225

0.485
  pilT Legionella pneumophila strain ERS1305867

49.547

97.929

0.485
  pilT Legionella pneumophila strain Lp02

49.547

97.929

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.338

97.929

0.473
  pilT Pseudomonas aeruginosa PAK

48.036

97.929

0.47
  pilT Acinetobacter baylyi ADP1

48.916

95.562

0.467
  pilT Acinetobacter baumannii D1279779

48.447

95.266

0.462
  pilT Acinetobacter baumannii strain A118

48.447

95.266

0.462
  pilT Acinetobacter nosocomialis M2

48.447

95.266

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.141

98.521

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.433

94.97

0.393
  pilU Vibrio cholerae strain A1552

38.416

100

0.388
  pilU Acinetobacter baylyi ADP1

39.13

95.266

0.373