Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   PJ250_RS13085 Genome accession   NZ_CP116339
Coordinates   2919917..2920351 (-) Length   144 a.a.
NCBI ID   WP_271645015.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. JBR18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2914917..2925351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJ250_RS13065 (PJ250_13065) - 2915227..2916090 (-) 864 WP_271645011.1 A24 family peptidase -
  PJ250_RS13070 (PJ250_13070) pilC 2916094..2917356 (-) 1263 WP_271645012.1 type II secretion system F family protein Machinery gene
  PJ250_RS13075 (PJ250_13075) pilB 2917547..2919283 (-) 1737 WP_271645013.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PJ250_RS13080 (PJ250_13080) pilA 2919364..2919780 (-) 417 WP_271645014.1 pilin Machinery gene
  PJ250_RS13085 (PJ250_13085) pilA2 2919917..2920351 (-) 435 WP_271645015.1 pilin Machinery gene
  PJ250_RS13090 (PJ250_13090) pilR 2920988..2922433 (-) 1446 WP_271645017.1 sigma-54 dependent transcriptional regulator Regulator
  PJ250_RS13095 (PJ250_13095) - 2922530..2924137 (-) 1608 WP_271645018.1 ATP-binding protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14818.92 Da        Isoelectric Point: 4.5873

>NTDB_id=776663 PJ250_RS13085 WP_271645015.1 2919917..2920351(-) (pilA2) [Pseudoxanthomonas sp. JBR18]
MKKNMQGGFTLIELMIVVAIIAILAAIALPAYQDYTIRAKVSEGLVQADAAKLAVSEAAQSLGGLANVTTDNAGFSLKDD
ATDYVKSVAIADGGEITVTTKATGATTDPVLVLTPTQADNDAPITWVCTHTSGEAKHVPATCRG

Nucleotide


Download         Length: 435 bp        

>NTDB_id=776663 PJ250_RS13085 WP_271645015.1 2919917..2920351(-) (pilA2) [Pseudoxanthomonas sp. JBR18]
ATGAAGAAGAACATGCAGGGCGGTTTCACCCTCATCGAACTGATGATCGTGGTCGCCATCATCGCCATCCTGGCCGCCAT
CGCACTGCCGGCCTATCAGGATTACACCATTCGCGCCAAGGTGTCCGAAGGCTTGGTCCAGGCTGACGCAGCGAAGCTGG
CTGTGTCAGAAGCAGCGCAGTCGCTTGGTGGACTTGCGAACGTCACGACCGATAACGCAGGTTTCTCACTTAAAGATGAC
GCTACCGACTATGTGAAGAGCGTGGCGATCGCAGACGGAGGCGAGATCACCGTCACCACTAAGGCAACCGGCGCTACGAC
GGATCCCGTTCTGGTGCTGACCCCGACGCAGGCAGATAACGATGCGCCGATTACCTGGGTATGCACCCATACTTCGGGCG
AAGCGAAGCATGTTCCGGCTACCTGCCGCGGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)

Pilin (bacterial filament) (Pilin)

(38-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

56.429

97.222

0.549
  pilA2 Legionella pneumophila strain ERS1305867

55.714

97.222

0.542
  comP Acinetobacter baylyi ADP1

49.02

100

0.521
  pilA Ralstonia pseudosolanacearum GMI1000

43.86

100

0.521
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.234

100

0.486
  pilA Haemophilus influenzae Rd KW20

40.132

100

0.424
  pilA Haemophilus influenzae 86-028NP

41.958

99.306

0.417
  pilE Neisseria gonorrhoeae strain FA1090

37.342

100

0.41
  pilE Neisseria gonorrhoeae MS11

36.943

100

0.403
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.278

100

0.403
  pilA/pilA1 Eikenella corrodens VA1

37.013

100

0.396
  pilA Pseudomonas aeruginosa PAK

37.086

100

0.389