Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PJ250_RS13075 Genome accession   NZ_CP116339
Coordinates   2917547..2919283 (-) Length   578 a.a.
NCBI ID   WP_271645013.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. JBR18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2912547..2924283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJ250_RS13060 (PJ250_13060) - 2914106..2915110 (+) 1005 WP_271645010.1 class I fructose-bisphosphate aldolase -
  PJ250_RS13065 (PJ250_13065) - 2915227..2916090 (-) 864 WP_271645011.1 A24 family peptidase -
  PJ250_RS13070 (PJ250_13070) pilC 2916094..2917356 (-) 1263 WP_271645012.1 type II secretion system F family protein Machinery gene
  PJ250_RS13075 (PJ250_13075) pilB 2917547..2919283 (-) 1737 WP_271645013.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PJ250_RS13080 (PJ250_13080) pilA 2919364..2919780 (-) 417 WP_271645014.1 pilin Machinery gene
  PJ250_RS13085 (PJ250_13085) pilA2 2919917..2920351 (-) 435 WP_271645015.1 pilin Machinery gene
  PJ250_RS13090 (PJ250_13090) pilR 2920988..2922433 (-) 1446 WP_271645017.1 sigma-54 dependent transcriptional regulator Regulator
  PJ250_RS13095 (PJ250_13095) - 2922530..2924137 (-) 1608 WP_271645018.1 ATP-binding protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62983.23 Da        Isoelectric Point: 5.9563

>NTDB_id=776661 PJ250_RS13075 WP_271645013.1 2917547..2919283(-) (pilB) [Pseudoxanthomonas sp. JBR18]
MNAVATSQTPNLVGITGIARRLVMDGALEEGAARTAMAEATQAKVPLAQWIQEKKLVAPAQLAAANAIEFGIPLIDASAF
DASQNALKLVSEELLQKHQVLPLFKRGNKLYVGVSDPTKMQALDEIKFHTNLVVEPILVADDQLKRTLEAWSNASDSLGG
LGGDEDGLENLDTSGGDDDISGDTGIDAKGEDTPVVKFVNKVLVDAIRRGASDIHFEPYETDYRVRLRIDGILKNVAKAP
QKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPDQQK
LFMDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPHDIP
PHALLAEGFTQQELDEGFTVYEPVGCDECTEGYKGRLGIYQVMPMTDEIATIVLEGGGAIQIAEAAQRSKIRDLRQSALW
KVKQGLTGLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=776661 PJ250_RS13075 WP_271645013.1 2917547..2919283(-) (pilB) [Pseudoxanthomonas sp. JBR18]
GTGAACGCTGTCGCCACCAGTCAAACGCCTAACTTGGTCGGCATTACCGGGATTGCCCGCCGACTGGTCATGGACGGCGC
CTTGGAGGAGGGCGCTGCGCGCACTGCGATGGCGGAGGCCACGCAGGCCAAAGTGCCGCTGGCGCAGTGGATCCAAGAAA
AAAAGCTCGTTGCGCCTGCGCAGCTCGCGGCAGCCAACGCGATCGAATTCGGCATCCCCCTCATCGATGCCAGCGCCTTC
GACGCCTCCCAGAACGCGCTCAAGCTGGTCAGCGAGGAACTGCTTCAGAAGCATCAGGTACTGCCACTGTTCAAGCGCGG
CAACAAGCTCTATGTCGGCGTCTCCGATCCGACCAAGATGCAGGCGCTGGATGAGATCAAGTTCCACACCAATTTGGTGG
TGGAGCCGATCCTGGTCGCCGACGATCAGCTCAAGCGCACGCTGGAGGCCTGGAGCAATGCGTCTGACTCCCTCGGCGGG
TTGGGCGGGGATGAGGATGGCCTTGAAAACCTGGACACCAGTGGCGGCGATGACGACATCAGCGGCGATACCGGCATCGA
TGCCAAGGGCGAAGACACCCCCGTGGTCAAGTTCGTCAACAAGGTGCTGGTCGACGCCATCCGTCGCGGCGCGTCGGACA
TCCATTTCGAGCCCTACGAAACCGACTATCGGGTGCGCTTGCGCATCGACGGCATCCTGAAGAATGTCGCCAAGGCGCCG
CAGAAGCTCAATCAGCGCATCGCCGCCCGCCTGAAGGTCATGTCCCAGCTGGACATCGCGGAAAAGCGCGTCCCCCAGGA
CGGGCGTATCAAGCTCAACCTCTCCAAGACCCGGCAGATCGACTTCCGCGTCAGCACTCTGCCGACTCTGTTCGGCGAGA
AGATCGTGCTGCGTATCCTGGACGGCAGCGCCGCCAAGCTGGGCATCGACAAGCTAGGTTACGAGCCAGACCAGCAAAAG
CTGTTCATGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCCGGCAAGACCGTGTCGCT
GTATACCGCGCTCGGCATCCTGAACGATGAAACGCGCAACATTTCCACCGCCGAGGACCCGGTGGAAATCCGCCTGCCGG
GCGTCAACCAGGTCCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCACTGCGCAGCTTTCTGCGTCAGGACCCC
GACATCATCATGGTCGGCGAGATCCGCGACCTGGAAACTGCGGAAATCGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTGTCCACCCTGCATACCAACGATGCCCCGCAGACCATTGCCCGCCTGATGAACATGGGTATCGCGCCCTACAACATCA
CCAGCTCGGTCACCTTGGTCATCGCCCAACGCTTGGCCCGACGCCTGTGCTCCAACTGCAAGCGTCCCCACGACATTCCT
CCGCACGCCCTGCTGGCCGAAGGATTCACCCAGCAAGAGCTGGACGAAGGTTTCACCGTCTACGAGCCGGTGGGCTGCGA
TGAGTGCACTGAAGGCTACAAGGGCCGGCTGGGCATCTATCAGGTCATGCCGATGACCGACGAGATCGCCACGATCGTGC
TGGAAGGCGGTGGTGCCATCCAGATCGCCGAGGCCGCCCAGCGCTCTAAGATCCGCGACCTGCGCCAATCGGCGCTGTGG
AAGGTCAAGCAGGGGTTAACCGGGCTGGCCGAGATCAATCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.862

100

0.561
  pilB Acinetobacter baylyi ADP1

55.07

98.962

0.545
  pilB Legionella pneumophila strain ERS1305867

52.381

98.097

0.514
  pilB Vibrio cholerae strain A1552

49.912

98.097

0.49
  pilF Neisseria gonorrhoeae MS11

48.763

97.924

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

46.655

95.675

0.446
  pilB Vibrio campbellii strain DS40M4

49.318

88.754

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.139

92.388

0.362