Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KK030_RS03800 Genome accession   NZ_CP116249
Coordinates   765140..766120 (+) Length   326 a.a.
NCBI ID   WP_063452722.1    Uniprot ID   -
Organism   Enterobacter roggenkampii strain 120063     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 760140..771120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KK030_RS03765 (KK030_03760) - 760518..760844 (+) 327 WP_003862421.1 YggL family protein -
  KK030_RS03770 (KK030_03765) - 760901..761617 (+) 717 WP_008499762.1 DUF2884 domain-containing protein -
  KK030_RS03775 (KK030_03770) hemW 761802..762950 (-) 1149 WP_063435943.1 radical SAM family heme chaperone HemW -
  KK030_RS03780 (KK030_03775) - 762943..763536 (-) 594 WP_023294524.1 XTP/dITP diphosphatase -
  KK030_RS03785 (KK030_03780) yggU 763540..763836 (-) 297 WP_008499758.1 DUF167 family protein YggU -
  KK030_RS03790 (KK030_03785) - 763833..764399 (-) 567 WP_008499757.1 YggT family protein -
  KK030_RS03795 (KK030_03790) - 764421..765122 (-) 702 WP_214574757.1 YggS family pyridoxal phosphate-dependent enzyme -
  KK030_RS03800 (KK030_03795) pilT 765140..766120 (+) 981 WP_063452722.1 type IV pilus twitching motility protein PilT Machinery gene
  KK030_RS03805 (KK030_03800) ruvX 766131..766547 (-) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  KK030_RS03810 (KK030_03805) - 766547..767107 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  KK030_RS03815 (KK030_03810) gshB 767190..768137 (-) 948 WP_214574758.1 glutathione synthase -
  KK030_RS03820 (KK030_03815) rsmE 768157..768888 (-) 732 WP_023344870.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KK030_RS03825 (KK030_03820) endA 768940..769647 (-) 708 WP_021242074.1 deoxyribonuclease I -
  KK030_RS03830 (KK030_03825) - 769742..770239 (-) 498 WP_021242073.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35483.43 Da        Isoelectric Point: 6.6962

>NTDB_id=776373 KK030_RS03800 WP_063452722.1 765140..766120(+) (pilT) [Enterobacter roggenkampii strain 120063]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPSPDLGALLKAWLNDEQQGIWWANGQVDFAATVAGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLASLGAPRAIPELLTNASGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLVPDLQDGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=776373 KK030_RS03800 WP_063452722.1 765140..766120(+) (pilT) [Enterobacter roggenkampii strain 120063]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACCGGCCGCCTTGAACCGGCACCGTTTCCTTCTCCGGACCTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAACGGGCAGGTGGATTTTGCCGCGACGGTGGCGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGAGTCTCTGTGACGCTGCGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCGCTGGG
CGCGCCGAGGGCAATCCCGGAACTGTTAACCAATGCGTCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGCCATATTCTCACGCTCGAAGATCCGGTGGAG
TTTATCTATCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATCGGCCAGCATAGCCCGTCATTTGCCGATGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTGATTTTGCTGGGAGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CAGCGGAGACCGGGCACCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAAGCTGGTTCC
TGACTTACAGGACGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACCGCCGCGGCGGCGAATCTGATACGAGAAGGAA
AAACGTGGCAGCTGCCCGGCATTATTCAAACCGGACAGCAGGCAGGCATGCAGAACTTTGACCAAAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.963

99.08

0.436
  pilU Pseudomonas stutzeri DSM 10701

39.879

100

0.405
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365