Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PCP76_RS01720 Genome accession   NZ_CP115297
Coordinates   342980..343435 (+) Length   151 a.a.
NCBI ID   WP_033976389.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 135     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 337980..348435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP76_RS01695 (PCP76_01690) yacG 338143..338343 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PCP76_RS01700 (PCP76_01695) coaE 338340..338951 (-) 612 WP_033970460.1 dephospho-CoA kinase -
  PCP76_RS01705 (PCP76_01700) pilD 338948..339820 (-) 873 WP_033976386.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP76_RS01710 (PCP76_01705) pilC 339824..341044 (-) 1221 WP_073649708.1 type 4a pilus biogenesis protein PilC Machinery gene
  PCP76_RS01715 (PCP76_01710) pilB 341048..342748 (-) 1701 WP_033976388.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP76_RS01720 (PCP76_01715) pilA 342980..343435 (+) 456 WP_033976389.1 pilin Machinery gene
  PCP76_RS01730 (PCP76_01725) nadC 343624..344472 (-) 849 WP_073649707.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PCP76_RS01735 (PCP76_01730) - 344758..347013 (+) 2256 WP_073649706.1 DUF1631 domain-containing protein -
  PCP76_RS01740 (PCP76_01735) ampD 347163..347729 (+) 567 WP_039028111.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15862.20 Da        Isoelectric Point: 7.7840

>NTDB_id=771607 PCP76_RS01720 WP_033976389.1 342980..343435(+) (pilA) [Pseudomonas aeruginosa strain 135]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASINPLKTTVEEALSRGWSVRSGTGAEDAAKKEVPLG
VDGKANNLGTIALNPDPANGAADITLTFTMDAAGPKNKDKVITLTRTATDGLWKCTSDQEKQFIPKGCSDK

Nucleotide


Download         Length: 456 bp        

>NTDB_id=771607 PCP76_RS01720 WP_033976389.1 342980..343435(+) (pilA) [Pseudomonas aeruginosa strain 135]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAATACCAGAACTATGTTGCGCGCTCGGAAGGCGCTTCGGCGCTGGCGTCCATCAACCCGTTGAAGACAACTGTAG
AAGAGGCTCTTTCTCGTGGTTGGAGCGTTAGGAGTGGTACTGGTGCAGAAGACGCTGCTAAAAAAGAAGTTCCGCTGGGT
GTAGATGGAAAGGCCAATAATCTCGGCACCATTGCTCTGAACCCTGATCCTGCAAATGGTGCTGCTGATATTACTCTGAC
CTTCACCATGGATGCTGCTGGTCCGAAGAACAAAGACAAAGTCATTACTCTGACTCGTACTGCTACTGATGGCCTTTGGA
AGTGCACCAGTGATCAGGAAAAGCAGTTCATTCCGAAAGGCTGCTCTGACAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

87.919

98.675

0.868
  pilA Acinetobacter baumannii strain A118

44.371

100

0.444
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44

99.338

0.437
  pilA Vibrio cholerae strain A1552

44

99.338

0.437
  pilA Vibrio cholerae C6706

44

99.338

0.437
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.421

100

0.437
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.205

96.689

0.437
  pilA Vibrio parahaemolyticus RIMD 2210633

47.761

88.742

0.424
  comP Acinetobacter baylyi ADP1

41.333

99.338

0.411
  pilA2 Legionella pneumophila str. Paris

40.136

97.351

0.391
  pilA2 Legionella pneumophila strain ERS1305867

39.456

97.351

0.384
  pilA/pilA1 Eikenella corrodens VA1

36.25

100

0.384
  pilA Acinetobacter nosocomialis M2

46.61

78.146

0.364