Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PCP76_RS01710 Genome accession   NZ_CP115297
Coordinates   339824..341044 (-) Length   406 a.a.
NCBI ID   WP_073649708.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 135     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 334824..346044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP76_RS01665 (PCP76_01660) - 335005..335454 (-) 450 WP_033970456.1 MOSC domain-containing protein -
  PCP76_RS01670 (PCP76_01665) - 335451..336080 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  PCP76_RS01675 (PCP76_01670) - 336213..336638 (+) 426 WP_033976385.1 GNAT family N-acetyltransferase -
  PCP76_RS01680 (PCP76_01675) - 336635..337105 (+) 471 WP_073649710.1 FAD/FMN-containing dehydrogenase -
  PCP76_RS01685 (PCP76_01680) - 337102..337791 (+) 690 WP_031805639.1 energy-coupling factor ABC transporter permease -
  PCP76_RS01690 (PCP76_01685) - 337897..338106 (+) 210 WP_073649709.1 hypothetical protein -
  PCP76_RS01695 (PCP76_01690) yacG 338143..338343 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PCP76_RS01700 (PCP76_01695) coaE 338340..338951 (-) 612 WP_033970460.1 dephospho-CoA kinase -
  PCP76_RS01705 (PCP76_01700) pilD 338948..339820 (-) 873 WP_033976386.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP76_RS01710 (PCP76_01705) pilC 339824..341044 (-) 1221 WP_073649708.1 type 4a pilus biogenesis protein PilC Machinery gene
  PCP76_RS01715 (PCP76_01710) pilB 341048..342748 (-) 1701 WP_033976388.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP76_RS01720 (PCP76_01715) pilA 342980..343435 (+) 456 WP_033976389.1 pilin Machinery gene
  PCP76_RS01730 (PCP76_01725) nadC 343624..344472 (-) 849 WP_073649707.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44316.09 Da        Isoelectric Point: 9.8784

>NTDB_id=771605 PCP76_RS01710 WP_073649708.1 339824..341044(-) (pilC) [Pseudomonas aeruginosa strain 135]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGVIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=771605 PCP76_RS01710 WP_073649708.1 339824..341044(-) (pilC) [Pseudomonas aeruginosa strain 135]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGCTGGTAGATGA
GATCAAGCAAGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCGCAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTGCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGCGTGGGGGTGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCGAAAAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTAGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAATCCGG
TTCGCTCGACGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baumannii D1279779

60.644

99.507

0.603
  pilC Acinetobacter baylyi ADP1

60.345

100

0.603
  pilC Legionella pneumophila strain ERS1305867

54.293

97.537

0.53
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.365

100

0.424
  pilC Vibrio campbellii strain DS40M4

41.604

98.276

0.409
  pilC Thermus thermophilus HB27

36.908

98.768

0.365