Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PCP76_RS01715 Genome accession   NZ_CP115297
Coordinates   341048..342748 (-) Length   566 a.a.
NCBI ID   WP_033976388.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 135     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 336048..347748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP76_RS01675 (PCP76_01670) - 336213..336638 (+) 426 WP_033976385.1 GNAT family N-acetyltransferase -
  PCP76_RS01680 (PCP76_01675) - 336635..337105 (+) 471 WP_073649710.1 FAD/FMN-containing dehydrogenase -
  PCP76_RS01685 (PCP76_01680) - 337102..337791 (+) 690 WP_031805639.1 energy-coupling factor ABC transporter permease -
  PCP76_RS01690 (PCP76_01685) - 337897..338106 (+) 210 WP_073649709.1 hypothetical protein -
  PCP76_RS01695 (PCP76_01690) yacG 338143..338343 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PCP76_RS01700 (PCP76_01695) coaE 338340..338951 (-) 612 WP_033970460.1 dephospho-CoA kinase -
  PCP76_RS01705 (PCP76_01700) pilD 338948..339820 (-) 873 WP_033976386.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP76_RS01710 (PCP76_01705) pilC 339824..341044 (-) 1221 WP_073649708.1 type 4a pilus biogenesis protein PilC Machinery gene
  PCP76_RS01715 (PCP76_01710) pilB 341048..342748 (-) 1701 WP_033976388.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP76_RS01720 (PCP76_01715) pilA 342980..343435 (+) 456 WP_033976389.1 pilin Machinery gene
  PCP76_RS01730 (PCP76_01725) nadC 343624..344472 (-) 849 WP_073649707.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PCP76_RS01735 (PCP76_01730) - 344758..347013 (+) 2256 WP_073649706.1 DUF1631 domain-containing protein -
  PCP76_RS01740 (PCP76_01735) ampD 347163..347729 (+) 567 WP_039028111.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62311.21 Da        Isoelectric Point: 5.7028

>NTDB_id=771606 PCP76_RS01715 WP_033976388.1 341048..342748(-) (pilB) [Pseudomonas aeruginosa strain 135]
MNDSIQLSGLSRQLVQANLLDEKTALQAQAQAQRNKLALVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=771606 PCP76_RS01715 WP_033976388.1 341048..342748(-) (pilB) [Pseudomonas aeruginosa strain 135]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAAACCGCCCTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGGCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAGAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGGCGCGGCAACAAGCTCTTCGTGGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAACTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGCTCGCCAGCCGCATCTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTGTACACCGGCCTGAACATCCTGAATACCACCGA
TATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCTTGCGCCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCTATCAAGGCGGCACAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.867

100

0.585
  pilB Acinetobacter baylyi ADP1

57.194

99.47

0.569
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

50.709

99.647

0.505
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio campbellii strain DS40M4

49.291

99.647

0.491
  pilB Vibrio cholerae strain A1552

52.767

89.399

0.472
  pilF Thermus thermophilus HB27

40.253

97.88

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.417

93.11

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

38.951

94.346

0.367