Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PCP64_RS11500 Genome accession   NZ_CP115276
Coordinates   2457638..2458672 (+) Length   344 a.a.
NCBI ID   WP_126861445.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F013     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2452638..2463672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP64_RS11475 (PCP64_11475) - 2452926..2454893 (-) 1968 WP_003121751.1 dynamin-like GTPase family protein -
  PCP64_RS11480 (PCP64_11480) - 2455001..2455297 (-) 297 WP_031628111.1 DUF167 domain-containing protein -
  PCP64_RS11485 (PCP64_11485) - 2455294..2455887 (-) 594 WP_003084544.1 YggT family protein -
  PCP64_RS11490 (PCP64_11490) proC 2455898..2456719 (-) 822 WP_003084546.1 pyrroline-5-carboxylate reductase -
  PCP64_RS11495 (PCP64_11495) - 2456731..2457423 (-) 693 WP_023122937.1 YggS family pyridoxal phosphate-dependent enzyme -
  PCP64_RS11500 (PCP64_11500) pilT 2457638..2458672 (+) 1035 WP_126861445.1 type IV pilus twitching motility protein PilT Machinery gene
  PCP64_RS11505 (PCP64_11505) pilU 2458850..2459998 (+) 1149 WP_003110016.1 type IV pilus ATPase PilU Machinery gene
  PCP64_RS11510 (PCP64_11510) czcD 2460006..2460905 (-) 900 WP_003100957.1 CDF family zinc transporter CzcD -
  PCP64_RS11515 (PCP64_11515) - 2461059..2461463 (+) 405 WP_003084560.1 NINE protein -
  PCP64_RS11520 (PCP64_11520) - 2461706..2463079 (+) 1374 WP_003100955.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38062.97 Da        Isoelectric Point: 6.8364

>NTDB_id=771103 PCP64_RS11500 WP_126861445.1 2457638..2458672(+) (pilT) [Pseudomonas aeruginosa strain F013]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGEVFKRVSDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQSVISQTLIKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KVLVAKGLISRENAREKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=771103 PCP64_RS11500 WP_126861445.1 2457638..2458672(+) (pilT) [Pseudomonas aeruginosa strain F013]
ATGGATATTACCGAGCTGCTCGCCTTCAGTGCCAAACAGGGCGCTTCGGACCTGCACCTCTCCGCCGGCCTGCCGCCCAT
GATCCGGGTGGATGGCGATGTACGCCGGATCAACCTGCCACCGCTGGAACACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAATTCCTCGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTATTCCGGACCATTCCCTCCAAGGTACTGACCATGGA
GGAGCTTGGCATGGGAGAAGTGTTCAAACGTGTTTCAGACGTCCCGCGCGGGTTGGTACTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCGATGCTCGATTACCTGAACAACACCAAGTACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCTCGGCTTCAGCGA
AGCGCTGCGCTCGGCGCTGCGGGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CCCTGACCGCGGCGGAGACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCGAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAAAAGGCCATGGTTCGCTCGATGCTCTCCGAGTCGCTGCAATCGGTGATCTCGCAGAC
CCTGATCAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGTGGCTCGCTGGGCATGCAGACCCTTGACATGTGCCTC
AAGGTCCTGGTCGCCAAGGGCCTGATCAGCCGCGAGAATGCCCGCGAGAAGGCGAAGATCCCGGAAAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

99.709

100

0.997
  pilT Pseudomonas stutzeri DSM 10701

93.895

100

0.939
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

81.686

100

0.817
  pilT Legionella pneumophila strain Lp02

74.556

98.256

0.733
  pilT Legionella pneumophila strain ERS1305867

74.556

98.256

0.733
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.765

98.837

0.66
  pilT Vibrio cholerae strain A1552

66.765

98.837

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

41.298

98.547

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395