Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   O1A01_RS19095 Genome accession   NZ_CP115153
Coordinates   3643887..3644924 (-) Length   345 a.a.
NCBI ID   WP_066414386.1    Uniprot ID   A0A223KTG1
Organism   Sutcliffiella sp. NC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3638887..3649924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1A01_RS19065 (O1A01_19065) - 3639106..3639804 (-) 699 WP_270064753.1 pilus assembly protein PilO -
  O1A01_RS19070 (O1A01_19070) - 3639801..3640388 (-) 588 WP_270064754.1 PilN domain-containing protein -
  O1A01_RS19075 (O1A01_19075) pilM 3640390..3641370 (-) 981 WP_270064755.1 pilus assembly protein PilM -
  O1A01_RS19080 (O1A01_19080) - 3641399..3642148 (-) 750 WP_270064756.1 A24 family peptidase -
  O1A01_RS19085 (O1A01_19085) - 3642152..3642583 (-) 432 WP_270064757.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  O1A01_RS19090 (O1A01_19090) - 3642675..3643883 (-) 1209 WP_270064758.1 type II secretion system F family protein -
  O1A01_RS19095 (O1A01_19095) pilT 3643887..3644924 (-) 1038 WP_066414386.1 type IV pilus twitching motility protein PilT Machinery gene
  O1A01_RS19100 (O1A01_19100) - 3644938..3646605 (-) 1668 WP_066414388.1 ATPase, T2SS/T4P/T4SS family -
  O1A01_RS19105 (O1A01_19105) - 3646628..3648004 (-) 1377 WP_270064759.1 hypothetical protein -
  O1A01_RS19110 (O1A01_19110) - 3648353..3649708 (-) 1356 WP_270064760.1 type II secretion system protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38542.36 Da        Isoelectric Point: 7.0344

>NTDB_id=768362 O1A01_RS19095 WP_066414386.1 3643887..3644924(-) (pilT) [Sutcliffiella sp. NC1]
MINEFQQWLYDAYSLKASDIHLSVGVPPVFRVNGELKRQGETILLPDDTNEIAKHMIPTHLWEVFQERGELDFSYSLPGV
SRFRVNAYRQRSSIALAIRIIPTSVPSIEELQLPPTIKKLVEQPQGLILVTGPTGSGKSTTLASMIDFMNRTKKKHIITL
EDPIEYLHKHKLCMIDQREIGFDTKSFANGLRASLRQDPDVILVGELRDLETIQTAITAAETGHLVLGTLHTTNAPSTID
RIIDVFPGHQQAQIRLQLASVLVAIVSQRLFPSIDKQSRKVATEILINNAAVANLIRNEKINQIQNVMQTSRAQGMHTLA
MSIQELVQAGLVSSQDVQSYLQESV

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=768362 O1A01_RS19095 WP_066414386.1 3643887..3644924(-) (pilT) [Sutcliffiella sp. NC1]
ATGATTAATGAATTTCAGCAATGGCTATATGATGCATATTCCTTGAAAGCTTCGGATATCCATTTATCGGTAGGAGTTCC
TCCGGTATTCCGTGTTAATGGGGAACTTAAGCGGCAAGGAGAAACGATATTACTTCCTGACGATACGAACGAAATTGCTA
AACATATGATCCCTACGCACCTTTGGGAAGTTTTTCAGGAGCGAGGAGAATTGGATTTCTCGTACAGTTTACCAGGAGTT
TCAAGATTTCGTGTTAATGCATATCGGCAACGTTCATCGATCGCATTAGCGATTCGGATTATCCCAACTAGTGTTCCATC
AATTGAGGAACTTCAACTACCGCCAACAATTAAAAAGTTAGTGGAACAACCTCAAGGTTTAATACTAGTAACAGGGCCTA
CTGGTAGTGGGAAATCTACTACACTAGCTTCGATGATTGACTTCATGAACCGAACGAAGAAAAAGCATATTATTACGTTA
GAAGATCCAATAGAATATTTACACAAACATAAGCTATGCATGATAGATCAGAGGGAAATTGGATTTGATACAAAATCGTT
TGCTAACGGATTACGTGCGTCGTTACGACAAGATCCTGATGTCATTTTAGTAGGAGAGTTACGTGACTTAGAAACGATTC
AAACTGCAATTACAGCTGCTGAGACCGGACATCTTGTGCTAGGTACACTTCACACAACGAATGCGCCAAGTACGATTGAC
CGAATTATCGATGTGTTTCCAGGTCATCAGCAGGCACAAATCCGTCTACAGCTTGCATCTGTTTTAGTTGCAATTGTTTC
GCAACGATTGTTTCCATCTATTGATAAGCAAAGTAGAAAAGTAGCAACAGAAATACTCATTAATAATGCAGCGGTTGCTA
ATCTCATCCGTAACGAGAAAATTAATCAGATTCAAAATGTGATGCAGACAAGCCGTGCGCAAGGTATGCATACATTGGCT
ATGAGTATACAAGAACTTGTCCAAGCAGGTCTCGTATCATCTCAAGATGTTCAAAGTTATTTACAGGAGTCGGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A223KTG1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.628

91.884

0.493
  pilT Neisseria meningitidis 8013

50.15

96.522

0.484
  pilT Neisseria gonorrhoeae MS11

49.85

96.522

0.481
  pilT Pseudomonas aeruginosa PAK

47.416

95.362

0.452
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.687

96.232

0.449
  pilT Vibrio cholerae strain A1552

46.687

96.232

0.449
  pilT Acinetobacter baumannii strain A118

46.667

95.652

0.446
  pilT Acinetobacter nosocomialis M2

46.667

95.652

0.446
  pilT Acinetobacter baumannii D1279779

46.667

95.652

0.446
  pilT Legionella pneumophila strain Lp02

47.222

93.913

0.443
  pilT Legionella pneumophila strain ERS1305867

47.222

93.913

0.443
  pilT Pseudomonas stutzeri DSM 10701

46.605

93.913

0.438
  pilT Acinetobacter baylyi ADP1

45.679

93.913

0.429
  pilU Pseudomonas stutzeri DSM 10701

40.841

96.522

0.394
  pilU Acinetobacter baylyi ADP1

40.597

97.101

0.394
  pilU Vibrio cholerae strain A1552

40.12

96.812

0.388