Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ICA20_RS04320 Genome accession   NZ_CP115032
Coordinates   872426..873406 (+) Length   326 a.a.
NCBI ID   WP_038633604.1    Uniprot ID   A0AAU7IRA2
Organism   Citrobacter sp. C5_2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 867426..878406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICA20_RS04290 (ICA20_004290) ansB 867939..868985 (+) 1047 WP_038633619.1 L-asparaginase 2 -
  ICA20_RS04295 (ICA20_004295) hemW 869102..870238 (-) 1137 WP_038633617.1 radical SAM family heme chaperone HemW -
  ICA20_RS04300 (ICA20_004300) - 870231..870824 (-) 594 WP_038633614.1 XTP/dITP diphosphatase -
  ICA20_RS04305 (ICA20_004305) yggU 870832..871122 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  ICA20_RS04310 (ICA20_004310) - 871119..871685 (-) 567 WP_038633609.1 YggT family protein -
  ICA20_RS04315 (ICA20_004315) - 871704..872408 (-) 705 WP_038633606.1 YggS family pyridoxal phosphate-dependent enzyme -
  ICA20_RS04320 (ICA20_004320) pilT 872426..873406 (+) 981 WP_038633604.1 type IV pilus twitching motility protein PilT Machinery gene
  ICA20_RS04325 (ICA20_004325) ruvX 873403..873819 (-) 417 WP_038633601.1 Holliday junction resolvase RuvX -
  ICA20_RS04330 (ICA20_004330) - 873819..874382 (-) 564 WP_032940453.1 YqgE/AlgH family protein -
  ICA20_RS04335 (ICA20_004335) gshB 874494..875441 (-) 948 WP_038633598.1 glutathione synthase -
  ICA20_RS04340 (ICA20_004340) rsmE 875454..876185 (-) 732 WP_038633593.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ICA20_RS04345 (ICA20_004345) endA 876260..876967 (-) 708 WP_038633590.1 deoxyribonuclease I -
  ICA20_RS04350 (ICA20_004350) - 877062..877559 (-) 498 WP_038633587.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35910.20 Da        Isoelectric Point: 6.7577

>NTDB_id=767950 ICA20_RS04320 WP_038633604.1 872426..873406(+) (pilT) [Citrobacter sp. C5_2]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGNVEIAPFTTPDVETLLMCWLSEQQQVQWQKQGQIDFAITLTNSRRLRA
SAFVHQQGISLALRLLPLDCPCLDDLQTPAALPELLQSENGLILVTGATGSGKSTTLAAMVSYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAPEKDPVRNQLADSLRAVLSQKLEQDKQGGRVALFELLINTPAVGNLIREGKTHQLPGIMQTGQQTGMQTFTQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=767950 ICA20_RS04320 WP_038633604.1 872426..873406(+) (pilT) [Citrobacter sp. C5_2]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAATGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATACGCGGAAATGTCGAAATCGCGCCGTTTACCACGCCTGACGTAGAGACGTTGCTGATGTGCTGGCTCAGCG
AGCAACAACAGGTACAGTGGCAGAAACAGGGACAGATAGATTTTGCCATTACGCTGACGAACTCACGGCGTCTGCGTGCG
AGCGCATTTGTGCATCAGCAGGGGATCTCTCTGGCGCTAAGGCTATTGCCGCTTGATTGCCCATGTTTGGATGATCTCCA
GACTCCCGCCGCCTTACCTGAGCTGCTGCAAAGTGAAAATGGATTAATTCTGGTGACCGGCGCTACCGGCAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTGTCATATCTTAATCAGCACGTCGCGGGACATATTTTGACGCTGGAAGATCCCATTGAA
TATCGTTACACCAGCCAGCGTTGTCTCATTCAACAACGGGAGGTAGGCGTACACTGCGCTTCTTTCGCCGCCGGTTTGCG
CGGCGCGCTGCGCGAAGATCCTGATGTCATTCTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACACACGCGAGGGGCGGCTCAGGCTATTGCGCGGCTGGTAGATACC
TTTCCTGCACCGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAACA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCATCAACACGCCCGCCGTTGGTAACCTGATCCGTGAAGGGA
AAACGCATCAGCTACCCGGCATAATGCAAACCGGGCAACAGACAGGCATGCAGACCTTTACGCAAAGCCTGCAGCAGCGC
CAGGCGCAGGGACGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.652

98.773

0.451
  pilT Legionella pneumophila strain Lp02

45.652

98.773

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.81

98.466

0.402
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Acinetobacter baylyi ADP1

39.077

99.693

0.39
  pilU Vibrio cholerae strain A1552

40.323

95.092

0.383