Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IB196_RS03730 Genome accession   NZ_CP115031
Coordinates   749010..749990 (+) Length   326 a.a.
NCBI ID   WP_040232091.1    Uniprot ID   A0AAU7ICT9
Organism   Citrobacter sp. C6_1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 744010..754990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IB196_RS03700 (IB196_003705) ansB 744542..745588 (+) 1047 WP_149692375.1 L-asparaginase 2 -
  IB196_RS03705 (IB196_003710) hemW 745686..746822 (-) 1137 WP_188231908.1 radical SAM family heme chaperone HemW -
  IB196_RS03710 (IB196_003715) - 746815..747408 (-) 594 WP_005123283.1 XTP/dITP diphosphatase -
  IB196_RS03715 (IB196_003720) yggU 747416..747706 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  IB196_RS03720 (IB196_003725) - 747703..748269 (-) 567 WP_003825417.1 YggT family protein -
  IB196_RS03725 (IB196_003730) - 748288..748992 (-) 705 WP_188231909.1 YggS family pyridoxal phosphate-dependent enzyme -
  IB196_RS03730 (IB196_003735) pilT 749010..749990 (+) 981 WP_040232091.1 type IV pilus twitching motility protein PilT Machinery gene
  IB196_RS03735 (IB196_003740) ruvX 749987..750403 (-) 417 WP_006686843.1 Holliday junction resolvase RuvX -
  IB196_RS03740 (IB196_003745) - 750403..750966 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  IB196_RS03745 (IB196_003750) gshB 751142..752089 (-) 948 WP_188231910.1 glutathione synthase -
  IB196_RS03750 (IB196_003755) rsmE 752109..752840 (-) 732 WP_040232093.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IB196_RS03755 (IB196_003760) endA 752915..753622 (-) 708 WP_188231911.1 deoxyribonuclease I -
  IB196_RS03760 (IB196_003765) - 753717..754214 (-) 498 WP_188231912.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36050.30 Da        Isoelectric Point: 6.4971

>NTDB_id=767926 IB196_RS03730 WP_040232091.1 749010..749990(+) (pilT) [Citrobacter sp. C6_1]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVESAPFTAPDVENLLMCWLSEQQQVQWREQGQIDFAVTLMDSRRLRA
SAFTHQQGMSLALRLLPLECPLLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVNYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGVHCTSFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FAASEKDPVRNQLADSLRAVISQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=767926 IB196_RS03730 WP_040232091.1 749010..749990(+) (pilT) [Citrobacter sp. C6_1]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATACGCGGAAAAGTCGAAAGCGCACCGTTTACCGCGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTAATGGACTCCCGACGGCTGCGCGCC
AGCGCATTTACCCATCAACAGGGAATGTCGCTGGCGCTAAGACTGCTACCGCTGGAATGTCCTCTTTTAGACGATCTCCA
GCCTCCCGCGGTCATACCTGAACTGCTGCACAGTGAAAACGGATTGATTCTGGTGACGGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGAACTATCTCAATCAACACATTGAGGGGCATATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGTACACTGCACCTCTTTTGCCGCCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTGACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTAGACACC
TTTGCGGCATCAGAAAAAGATCCCGTGCGTAATCAACTGGCGGATAGCCTGCGGGCGGTTATTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAGCTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATACAAACCGGGCAGCAGACAGGTATGCAGACGTTCGCACAAAGTTTGCAGCAGCGC
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Acinetobacter baylyi ADP1

37.461

99.08

0.371