Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IB222_RS03795 Genome accession   NZ_CP115024
Coordinates   783548..784528 (+) Length   326 a.a.
NCBI ID   WP_149384570.1    Uniprot ID   A0AAU7HKN8
Organism   Citrobacter sp. C13     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 778548..789528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IB222_RS03765 (IB222_003760) ansB 779063..780109 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  IB222_RS03770 (IB222_003765) hemW 780224..781360 (-) 1137 WP_048236736.1 radical SAM family heme chaperone HemW -
  IB222_RS03775 (IB222_003770) - 781353..781946 (-) 594 WP_003825414.1 XTP/dITP diphosphatase -
  IB222_RS03780 (IB222_003775) yggU 781954..782244 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  IB222_RS03785 (IB222_003780) - 782241..782807 (-) 567 WP_003825417.1 YggT family protein -
  IB222_RS03790 (IB222_003785) - 782826..783530 (-) 705 WP_057067562.1 YggS family pyridoxal phosphate-dependent enzyme -
  IB222_RS03795 (IB222_003790) pilT 783548..784528 (+) 981 WP_149384570.1 type IV pilus twitching motility protein PilT Machinery gene
  IB222_RS03800 (IB222_003795) ruvX 784525..784941 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  IB222_RS03805 (IB222_003800) - 784941..785504 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  IB222_RS03810 (IB222_003805) gshB 785680..786627 (-) 948 WP_003027083.1 glutathione synthase -
  IB222_RS03815 (IB222_003810) rsmE 786647..787378 (-) 732 WP_003825432.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IB222_RS03820 (IB222_003815) endA 787453..788160 (-) 708 WP_008785767.1 deoxyribonuclease I -
  IB222_RS03825 (IB222_003820) - 788255..788752 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35941.12 Da        Isoelectric Point: 6.7587

>NTDB_id=767881 IB222_RS03795 WP_149384570.1 783548..784528(+) (pilT) [Citrobacter sp. C13]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTTPDVENLLMCWLSEQQQVQWREQGQVDFAIALAGSQRLRA
SAFVHQQGTSLALRLLPLDCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FSATEKDPVRNQLADSLRAVLSQKLEKDKQGGRVALFELLVNTPAVCNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=767881 IB222_RS03795 WP_149384570.1 783548..784528(+) (pilT) [Citrobacter sp. C13]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGGTAGATTTTGCTATTGCGCTGGCAGGTTCCCAGCGGCTGCGCGCC
AGTGCTTTTGTTCATCAACAGGGAACCTCACTGGCGCTAAGATTGCTCCCGCTTGATTGTCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTAGAGTACCTTAATCAGCATGTTGCAGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAACGGGAGGTGGGCGTACACTGCGCTTCTTTTGCCGCCGGTTTACG
CGGCGCGCTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAACTGCGCGACGCGGAAACCATTCGTCTGGCGTTAACGG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCACAGGCCATCGCGCGGCTGGTGGACACC
TTTTCGGCAACAGAGAAGGATCCGGTGCGTAACCAGTTAGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGAA
AGATAAGCAGGGAGGACGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCTGCCGTGTGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTGCCCGGCGTAATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGTCAGCAGCAGCGT
CAGGCGCAGGGACGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Neisseria meningitidis 8013

46.667

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.364

100

0.469
  pilT Acinetobacter baylyi ADP1

45.897

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Acinetobacter baumannii strain A118

45.593

100

0.46
  pilT Acinetobacter baumannii D1279779

45.593

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.289

100

0.457
  pilT Legionella pneumophila strain Lp02

46.855

97.546

0.457
  pilT Legionella pneumophila strain ERS1305867

46.855

97.546

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368