Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   O2T12_RS23010 Genome accession   NZ_CP114886
Coordinates   5530617..5531651 (-) Length   344 a.a.
NCBI ID   WP_163370308.1    Uniprot ID   -
Organism   Endozoicomonas sp. GU-1 strain Ap1-3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5525617..5536651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O2T12_RS22995 (O2T12_22995) rppH 5526316..5526807 (+) 492 WP_101744739.1 RNA pyrophosphohydrolase -
  O2T12_RS23000 (O2T12_23000) ptsP 5526993..5529269 (+) 2277 WP_269676575.1 phosphoenolpyruvate--protein phosphotransferase -
  O2T12_RS23005 (O2T12_23005) - 5529430..5530553 (-) 1124 Protein_4505 PilT/PilU family type 4a pilus ATPase -
  O2T12_RS23010 (O2T12_23010) pilT 5530617..5531651 (-) 1035 WP_163370308.1 type IV pilus twitching motility protein PilT Machinery gene
  O2T12_RS23015 (O2T12_23015) - 5531753..5532487 (+) 735 WP_269675976.1 YggS family pyridoxal phosphate-dependent enzyme -
  O2T12_RS23020 (O2T12_23020) proC 5532484..5533236 (+) 753 WP_269675865.1 pyrroline-5-carboxylate reductase -
  O2T12_RS23025 (O2T12_23025) - 5533388..5533942 (+) 555 WP_269675866.1 YggT family protein -
  O2T12_RS23030 (O2T12_23030) - 5534345..5536552 (+) 2208 WP_269675867.1 glycoside hydrolase domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38042.76 Da        Isoelectric Point: 6.8355

>NTDB_id=767375 O2T12_RS23010 WP_163370308.1 5530617..5531651(-) (pilT) [Endozoicomonas sp. GU-1 strain Ap1-3]
MDITELLAFSYKQGASDLHLSAGLPPIIRIDGDVRRINLPPMEQQEVHGLIYDIMNDKQRKDLEEHLEADFSFEVPGVAR
FRVNAFNQNRGAGAVFRAIPSKVLTMDDLGMGQVFKTIADKPRGLVLVTGPTGSGKSTTLAAMMDYINDTKYQHILTIED
PIEFIHTPKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKNGGGRVAAHEIMLGTAGIRNLIREDKVAQMYSAIQTGGGLGMQTLDQCL
TNLMKKGLISRDVAREKAKDRDAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=767375 O2T12_RS23010 WP_163370308.1 5530617..5531651(-) (pilT) [Endozoicomonas sp. GU-1 strain Ap1-3]
ATGGATATTACTGAGCTATTGGCGTTCAGCTATAAACAGGGGGCTTCGGATCTGCATCTGTCGGCAGGACTTCCGCCAAT
TATTCGAATCGACGGTGATGTACGCCGTATCAATCTGCCGCCCATGGAGCAGCAAGAAGTTCATGGTTTGATCTATGACA
TCATGAACGACAAACAGCGTAAAGATCTGGAAGAGCACCTGGAAGCGGACTTCTCGTTTGAAGTACCGGGCGTTGCCCGT
TTCCGCGTCAATGCTTTCAATCAAAACCGGGGAGCGGGGGCGGTGTTCCGGGCTATCCCCAGTAAAGTACTGACCATGGA
TGATCTGGGCATGGGACAGGTATTTAAAACCATTGCGGATAAACCCCGGGGGTTAGTGCTGGTGACTGGCCCTACCGGCT
CTGGCAAGAGCACCACGCTGGCGGCCATGATGGATTACATTAATGACACCAAATATCAGCATATCCTGACCATCGAAGAC
CCCATTGAATTTATCCACACCCCGAAAAAGTGTCTGGTTAACCAGCGGGAAGTGCATCGGGATACCCATAGCTTCAGCAA
TGCCCTGCGCTCTGCGTTGCGTGAAGATCCCGATATTATTCTGGTCGGTGAGATGCGTGACCTGGAAACCATCCGACTGG
CGATGACCGCTGCGGAAACCGGTCATTTGGTGTTCGGTACGTTGCACACCACCTCGGCGGCAAAAACCATTGACCGTATC
GTCGATGTATTTCCGGCGGAAGAGAAGTCCATGGTGCGTTCAATGCTCTCGGAATCACTCCAGGCTGTGATCTCGCAAAC
ACTTTTGAAGAAAAACGGTGGAGGCCGGGTAGCTGCCCATGAGATTATGCTTGGCACGGCAGGTATTCGAAACCTGATTC
GCGAAGATAAGGTTGCCCAGATGTACTCGGCCATTCAGACCGGCGGTGGGCTTGGCATGCAAACACTGGATCAGTGCCTG
ACGAACCTGATGAAAAAGGGACTGATCTCTCGTGATGTGGCCCGTGAAAAGGCCAAGGACCGTGACGCATTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

85.756

100

0.858
  pilT Pseudomonas aeruginosa PAK

85.465

100

0.855
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

72.965

100

0.73
  pilT Legionella pneumophila strain ERS1305867

72.965

100

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.667

100

0.669
  pilT Vibrio cholerae strain A1552

66.667

100

0.669
  pilT Neisseria meningitidis 8013

65.507

100

0.657
  pilT Neisseria gonorrhoeae MS11

64.928

100

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

41.088

96.221

0.395
  pilU Acinetobacter baylyi ADP1

40.06

96.512

0.387