Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QN220_RS04020 Genome accession   NZ_CP126343
Coordinates   822507..823487 (+) Length   326 a.a.
NCBI ID   WP_153443487.1    Uniprot ID   -
Organism   Escherichia coli strain DAX4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 817507..828487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN220_RS03990 (QN220_03990) yggM 818022..819029 (+) 1008 WP_000745210.1 DUF1202 family protein -
  QN220_RS03995 (QN220_03995) hemW 819184..820320 (-) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  QN220_RS04000 (QN220_04000) rdgB 820313..820906 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  QN220_RS04005 (QN220_04005) yggU 820914..821204 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  QN220_RS04010 (QN220_04010) yggT 821201..821767 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  QN220_RS04015 (QN220_04015) yggS 821785..822489 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  QN220_RS04020 (QN220_04020) pilT 822507..823487 (+) 981 WP_153443487.1 type IV pilus twitching motility protein PilT Machinery gene
  QN220_RS04025 (QN220_04025) - 823536..823664 (+) 129 WP_001278270.1 hypothetical protein -
  QN220_RS04030 (QN220_04030) ruvX 823671..824087 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  QN220_RS04035 (QN220_04035) yqgE 824087..824650 (-) 564 WP_001350133.1 YqgE/AlgH family protein -
  QN220_RS04040 (QN220_04040) gshB 824759..825709 (-) 951 WP_000593273.1 glutathione synthase -
  QN220_RS04045 (QN220_04045) rsmE 825722..826453 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QN220_RS04050 (QN220_04050) endA 826533..827240 (-) 708 WP_000286500.1 deoxyribonuclease I -
  QN220_RS04055 (QN220_04055) yggI 827335..827832 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35873.09 Da        Isoelectric Point: 5.5639

>NTDB_id=767093 QN220_RS04020 WP_153443487.1 822507..823487(+) (pilT) [Escherichia coli strain DAX4]
MNMEEIVALSVKHNVSDLHLCSAWPARWCIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=767093 QN220_RS04020 WP_153443487.1 822507..823487(+) (pilT) [Escherichia coli strain DAX4]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGTGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCATGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.597

97.546

0.445
  pilT Legionella pneumophila strain Lp02

45.597

97.546

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.968

95.092

0.39
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

37.048

100

0.377