Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   O4546_RS10900 Genome accession   NZ_CP114590
Coordinates   2380829..2381845 (-) Length   338 a.a.
NCBI ID   WP_269587060.1    Uniprot ID   -
Organism   Salinivibrio kushneri strain TGB19     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2375829..2386845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4546_RS10870 (O4546_10870) metK 2376207..2377361 (+) 1155 WP_269587056.1 methionine adenosyltransferase -
  O4546_RS10875 (O4546_10875) - 2377432..2377959 (+) 528 WP_269587057.1 SprT family zinc-dependent metalloprotease -
  O4546_RS10880 (O4546_10880) rsmE 2378058..2378789 (+) 732 WP_077649810.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  O4546_RS10885 (O4546_10885) gshB 2378808..2379752 (+) 945 WP_269587058.1 glutathione synthase -
  O4546_RS10890 (O4546_10890) - 2379841..2380407 (+) 567 WP_069362088.1 YqgE/AlgH family protein -
  O4546_RS10895 (O4546_10895) ruvX 2380404..2380832 (+) 429 WP_269587059.1 Holliday junction resolvase RuvX -
  O4546_RS10900 (O4546_10900) pilT 2380829..2381845 (-) 1017 WP_269587060.1 type IV pilus twitching motility protein PilT Machinery gene
  O4546_RS10905 (O4546_10905) - 2381869..2382570 (+) 702 WP_269587061.1 YggS family pyridoxal phosphate-dependent enzyme -
  O4546_RS10910 (O4546_10910) proC 2382625..2383443 (+) 819 WP_269587062.1 pyrroline-5-carboxylate reductase -
  O4546_RS10915 (O4546_10915) - 2383460..2384014 (+) 555 WP_069362083.1 YggT family protein -
  O4546_RS10920 (O4546_10920) - 2384169..2384609 (+) 441 WP_077522840.1 DUF4426 domain-containing protein -
  O4546_RS10925 (O4546_10925) - 2384638..2385234 (+) 597 WP_069362081.1 XTP/dITP diphosphatase -
  O4546_RS10930 (O4546_10930) hemW 2385238..2386389 (+) 1152 WP_269587063.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36822.28 Da        Isoelectric Point: 6.5001

>NTDB_id=766695 O4546_RS10900 WP_269587060.1 2380829..2381845(-) (pilT) [Salinivibrio kushneri strain TGB19]
MDINELLAFSVKHRASDLHLSAGVSPMVRVDGDMRKLSLPPLSHQAVRELVLAVMGPEQQHVFDNTWEVDFSFELNSVAR
FRVNAFHQLHGCAAVFRVVPTAVPSLEDLSAPPLFSELALLHQGLVLVTGPTGSGKSTTLAAMIAQINHHQQKHILTIED
PIEFIHPSEQSLVNQREVKRDTQSFQQALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTASAAKSVDRI
IDVFPGSDKPLVRSMLSSSLQAVISQQLVKSTQGQRVAAYEIMTVTSAVCNLIREDRVAQIASMMQTGTAHGMQTMAQAM
AQLEKQGVIAPQAAISPS

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=766695 O4546_RS10900 WP_269587060.1 2380829..2381845(-) (pilT) [Salinivibrio kushneri strain TGB19]
ATGGATATTAATGAGCTACTTGCGTTTAGTGTAAAGCATCGCGCGTCAGATCTACATCTTTCTGCGGGGGTGTCGCCTAT
GGTGCGGGTAGATGGCGATATGCGCAAGCTTAGTCTGCCACCGCTGAGCCACCAAGCCGTGCGGGAACTGGTACTGGCAG
TGATGGGCCCCGAGCAGCAGCATGTTTTTGACAACACATGGGAAGTCGACTTTTCCTTTGAACTAAACTCGGTGGCGCGT
TTTCGCGTCAATGCGTTCCACCAGCTACATGGCTGTGCGGCGGTCTTTCGTGTGGTGCCCACCGCCGTACCAAGTCTAGA
GGATTTATCCGCGCCGCCACTTTTCAGTGAGCTGGCTTTGTTACATCAAGGGTTGGTGTTGGTGACTGGGCCGACAGGCT
CGGGGAAGTCGACCACACTGGCGGCGATGATTGCGCAGATTAACCATCACCAACAAAAGCATATTCTGACTATCGAAGAT
CCGATTGAATTTATCCATCCGTCCGAGCAAAGCTTGGTGAACCAGCGCGAGGTCAAGCGTGATACGCAGTCTTTTCAGCA
AGCCTTGCGCTCAGCGCTGCGTGAAGACCCAGATGTGATATTAGTGGGCGAGCTACGAGACTTAGAAACCATCCGTCTAG
CGTTAACCGCGGCAGAGACCGGGCACTTGGTGTTTGCGACCTTGCACACCGCTAGCGCAGCGAAAAGTGTCGATCGTATC
ATTGATGTATTTCCCGGCAGCGATAAGCCCTTGGTGCGTTCGATGCTGTCGTCCTCACTGCAGGCGGTGATTTCTCAACA
ACTGGTCAAAAGCACCCAAGGTCAGCGTGTTGCTGCTTATGAAATTATGACGGTGACATCAGCGGTTTGTAACTTGATCC
GAGAGGATAGAGTGGCGCAAATCGCCTCCATGATGCAAACTGGTACCGCGCATGGTATGCAGACGATGGCGCAAGCGATG
GCCCAGCTCGAGAAACAAGGGGTGATTGCACCGCAGGCGGCGATCTCGCCCAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.867

98.225

0.657
  pilT Vibrio cholerae strain A1552

66.867

98.225

0.657
  pilT Neisseria gonorrhoeae MS11

62.388

99.112

0.618
  pilT Neisseria meningitidis 8013

62.388

99.112

0.618
  pilT Legionella pneumophila strain ERS1305867

61.976

98.817

0.612
  pilT Legionella pneumophila strain Lp02

61.976

98.817

0.612
  pilT Acinetobacter baumannii D1279779

61.677

98.817

0.609
  pilT Acinetobacter baumannii strain A118

61.677

98.817

0.609
  pilT Acinetobacter nosocomialis M2

61.677

98.817

0.609
  pilT Pseudomonas aeruginosa PAK

61.377

98.817

0.607
  pilT Pseudomonas stutzeri DSM 10701

61.078

98.817

0.604
  pilT Acinetobacter baylyi ADP1

61.094

97.337

0.595
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.606

97.633

0.494
  pilU Pseudomonas stutzeri DSM 10701

42.262

99.408

0.42
  pilU Acinetobacter baylyi ADP1

42.638

96.45

0.411
  pilU Vibrio cholerae strain A1552

40.476

99.408

0.402