Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N8M53_RS10875 Genome accession   NZ_CP114588
Coordinates   2360833..2361861 (-) Length   342 a.a.
NCBI ID   WP_269578793.1    Uniprot ID   A0AA47LR20
Organism   Salinivibrio kushneri strain TGB11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2355833..2366861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8M53_RS10845 (N8M53_10845) metK 2356223..2357377 (+) 1155 WP_077606492.1 methionine adenosyltransferase -
  N8M53_RS10850 (N8M53_10850) - 2357449..2357964 (+) 516 WP_269578789.1 SprT family zinc-dependent metalloprotease -
  N8M53_RS10855 (N8M53_10855) rsmE 2358075..2358806 (+) 732 WP_269578790.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N8M53_RS10860 (N8M53_10860) gshB 2358825..2359769 (+) 945 WP_269578791.1 glutathione synthase -
  N8M53_RS10865 (N8M53_10865) - 2359857..2360423 (+) 567 WP_269578792.1 YqgE/AlgH family protein -
  N8M53_RS10870 (N8M53_10870) ruvX 2360420..2360848 (+) 429 WP_046075064.1 Holliday junction resolvase RuvX -
  N8M53_RS10875 (N8M53_10875) pilT 2360833..2361861 (-) 1029 WP_269578793.1 type IV pilus twitching motility protein PilT Machinery gene
  N8M53_RS10880 (N8M53_10880) - 2361885..2362586 (+) 702 WP_269578794.1 YggS family pyridoxal phosphate-dependent enzyme -
  N8M53_RS10885 (N8M53_10885) proC 2362642..2363460 (+) 819 WP_269578795.1 pyrroline-5-carboxylate reductase -
  N8M53_RS10890 (N8M53_10890) - 2363477..2364031 (+) 555 WP_046075060.1 YggT family protein -
  N8M53_RS10895 (N8M53_10895) - 2364186..2364626 (+) 441 WP_077578285.1 DUF4426 domain-containing protein -
  N8M53_RS10900 (N8M53_10900) rdgB 2364655..2365251 (+) 597 WP_269578796.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  N8M53_RS10905 (N8M53_10905) hemW 2365255..2366406 (+) 1152 WP_269578797.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 37415.97 Da        Isoelectric Point: 6.4999

>NTDB_id=766664 N8M53_RS10875 WP_269578793.1 2360833..2361861(-) (pilT) [Salinivibrio kushneri strain TGB11]
MDINELLAFSVKHRASDLHLSAGVSPMVRVDGDMRKLSLPPLSHQAVRELVMAVMSPEQQHIFDNAWEIDFSFELDSVAR
FRVNAFHQQHGCAAVFRVVPTTVPRLEDLSAPPLFSELASLHQGLVLVTGPTGSGKSTTLAAMIAQINHHQQKHILTIED
PIEFIHPSLQSLVNQREVKRDTQSFQQALRSALREDPDVILVGELRDLETISLALTAAETGHLVFATLHTVSAAKSVDRI
IDVFPGSDKPLVRSMLSASLRAVISQQLVKSIQGQRVAAYEIMTVTSAICNLIREDRVAQITSMMQTGAAHGMQTMAQAM
AQLENQGVIAPQAAISPTQSVY

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=766664 N8M53_RS10875 WP_269578793.1 2360833..2361861(-) (pilT) [Salinivibrio kushneri strain TGB11]
ATGGATATTAATGAGCTACTTGCGTTTAGTGTAAAGCATCGCGCGTCAGATCTACATCTTTCTGCGGGGGTGTCGCCTAT
GGTGCGGGTAGATGGCGATATGCGCAAGCTCAGTCTGCCGCCGCTTAGCCACCAAGCCGTGCGCGAATTGGTAATGGCGG
TGATGAGCCCTGAACAGCAGCATATTTTTGACAATGCATGGGAAATCGACTTTTCCTTTGAGCTGGACTCGGTGGCACGC
TTTCGTGTTAATGCCTTCCATCAGCAACATGGCTGTGCGGCGGTTTTTCGCGTGGTGCCCACCACTGTACCAAGGTTAGA
GGATTTATCCGCGCCGCCACTTTTCAGTGAGCTGGCTTCGTTACACCAAGGGCTGGTGTTGGTGACTGGGCCGACAGGCT
CGGGGAAATCGACCACACTAGCGGCCATGATTGCCCAGATTAATCACCACCAACAAAAGCATATTCTGACTATTGAAGAT
CCGATTGAGTTTATCCATCCGTCTCTGCAAAGCTTGGTGAACCAGCGAGAGGTCAAGCGTGATACTCAGTCTTTTCAGCA
GGCATTGCGCTCAGCGCTGCGTGAAGACCCAGATGTGATATTGGTGGGTGAGTTACGTGATTTAGAAACCATCAGTTTGG
CATTAACGGCGGCAGAAACCGGGCATTTGGTGTTTGCGACTTTACATACCGTCAGCGCCGCTAAAAGTGTTGACCGTATT
ATTGATGTTTTTCCCGGTAGTGATAAACCCTTGGTACGTTCAATGCTCTCTGCCTCATTGCGGGCGGTGATCTCTCAGCA
GCTTGTCAAAAGCATCCAAGGTCAGCGTGTTGCCGCTTATGAAATTATGACGGTGACGTCAGCGATTTGTAACTTGATCC
GAGAGGATAGAGTGGCGCAAATCACCTCCATGATGCAAACGGGTGCCGCGCATGGCATGCAGACCATGGCACAAGCGATG
GCACAGCTAGAGAACCAAGGCGTGATTGCGCCGCAGGCGGCGATCTCGCCCACTCAATCTGTGTATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.771

97.076

0.658
  pilT Vibrio cholerae strain A1552

67.771

97.076

0.658
  pilT Neisseria meningitidis 8013

62.09

97.953

0.608
  pilT Neisseria gonorrhoeae MS11

62.09

97.953

0.608
  pilT Legionella pneumophila strain ERS1305867

61.976

97.661

0.605
  pilT Legionella pneumophila strain Lp02

61.976

97.661

0.605
  pilT Acinetobacter baumannii strain A118

61.677

97.661

0.602
  pilT Acinetobacter nosocomialis M2

61.677

97.661

0.602
  pilT Acinetobacter baumannii D1279779

61.677

97.661

0.602
  pilT Pseudomonas aeruginosa PAK

61.377

97.661

0.599
  pilT Pseudomonas stutzeri DSM 10701

60.778

97.661

0.594
  pilT Acinetobacter baylyi ADP1

61.094

96.199

0.588
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.16

94.737

0.494
  pilU Pseudomonas stutzeri DSM 10701

41.667

98.246

0.409
  pilU Acinetobacter baylyi ADP1

40.351

100

0.404
  pilU Vibrio cholerae strain A1552

40.469

99.708

0.404