Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N5B28_RS03840 Genome accession   NZ_CP114160
Coordinates   764681..765661 (+) Length   326 a.a.
NCBI ID   WP_023290994.1    Uniprot ID   -
Organism   Klebsiella quasipneumoniae strain 2022CK-00516     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 759681..770661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5B28_RS03810 (N5B28_03810) - 760063..761049 (-) 987 WP_023290997.1 TRAP transporter substrate-binding protein -
  N5B28_RS03815 (N5B28_03815) hemW 761354..762490 (-) 1137 WP_048298268.1 radical SAM family heme chaperone HemW -
  N5B28_RS03820 (N5B28_03820) - 762483..763076 (-) 594 WP_048298266.1 XTP/dITP diphosphatase -
  N5B28_RS03825 (N5B28_03825) yggU 763089..763379 (-) 291 WP_004205183.1 DUF167 family protein YggU -
  N5B28_RS03830 (N5B28_03830) - 763376..763942 (-) 567 WP_072421873.1 YggT family protein -
  N5B28_RS03835 (N5B28_03835) - 763963..764664 (-) 702 WP_004205181.1 YggS family pyridoxal phosphate-dependent enzyme -
  N5B28_RS03840 (N5B28_03840) pilT 764681..765661 (+) 981 WP_023290994.1 type IV pilus twitching motility protein PilT Machinery gene
  N5B28_RS03845 (N5B28_03845) - 766010..766663 (+) 654 WP_032454048.1 LuxR C-terminal-related transcriptional regulator -
  N5B28_RS03850 (N5B28_03850) ruvX 766715..767131 (-) 417 WP_101996192.1 Holliday junction resolvase RuvX -
  N5B28_RS03855 (N5B28_03855) - 767131..767694 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  N5B28_RS03860 (N5B28_03860) gshB 767869..768819 (-) 951 WP_004205176.1 glutathione synthase -
  N5B28_RS03865 (N5B28_03865) rsmE 768840..769571 (-) 732 WP_023290991.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N5B28_RS03870 (N5B28_03870) endA 769663..770370 (-) 708 WP_032454045.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35413.82 Da        Isoelectric Point: 6.8575

>NTDB_id=764564 N5B28_RS03840 WP_023290994.1 764681..765661(+) (pilT) [Klebsiella quasipneumoniae strain 2022CK-00516]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLESAPFPAPDIADLLNDWLDAAQFQHWQEHGQIDFALTLACGSRLRA
SAFAHTRGISLVLRLLPVQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFSAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VATGQL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=764564 N5B28_RS03840 WP_023290994.1 764681..765661(+) (pilT) [Klebsiella quasipneumoniae strain 2022CK-00516]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGTCCGCTCCCTTTCCCGCGCCGGATATCGCGGATTTACTCAACGACTGGCTCGATG
CCGCACAGTTCCAGCACTGGCAGGAGCACGGCCAGATTGACTTTGCGCTGACTCTGGCCTGCGGTTCGCGGCTACGCGCC
AGCGCTTTCGCCCATACGCGCGGAATATCGCTGGTGCTGCGTCTGCTGCCCGTCCAGTGCCCGCGTCTGGAGACGCTGGG
CGCGCCGCCTGCGCTAAGCGAGCTGCTGGCGGAAGAGAGTGGCCTGCTGCTGGTCACCGGGGCGACGGGCAGCGGCAAGT
CGACTACCCTGGCGGCGATGGTCGGGCATCTCAACCAGCATCTTGATGGCCATATTCTGACGCTGGAGGATCCGGTGGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCAGCAGCGGGAGATTGGCCGTCACTGTCCTTCGTTCTCCGCGGCGCTTCG
GGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTTGGGGAGTTACGCGACAGCGAAACCATCCGTCTGGCGTTGACGG
CTGCGGAAACCGGGCATCTGGTGATGGCGACATTACACACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGGAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTATTACC
CGCACGTCAGGGCGGCAGAGTTGCGCTATACGAGCTTCTGGTCAATACGCCGGCGGTGGCGAATTTGATCCGCGAAGGAA
AAGTGCACCAGCTGCCCGGTGTAATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACCCAGAGTTTTCAGCAACGT
GTGGCCACAGGCCAGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.682

100

0.518
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.682

100

0.518
  pilT Acinetobacter baylyi ADP1

48.624

100

0.488
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

47.706

100

0.479
  pilT Legionella pneumophila strain ERS1305867

47.84

99.387

0.475
  pilT Legionella pneumophila strain Lp02

47.84

99.387

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.164

97.239

0.429
  pilU Vibrio cholerae strain A1552

40.426

100

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.252

98.466

0.386