Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OZ428_RS31230 Genome accession   NZ_CP114115
Coordinates   6598150..6599184 (-) Length   344 a.a.
NCBI ID   WP_269132233.1    Uniprot ID   -
Organism   Pseudomonas sp. GXZC     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6593150..6604184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OZ428_RS31200 (OZ428_31200) ruvX 6593215..6593652 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  OZ428_RS31205 (OZ428_31205) pyrR 6593726..6594232 (+) 507 WP_033897063.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  OZ428_RS31210 (OZ428_31210) - 6594257..6595261 (+) 1005 WP_016976042.1 aspartate carbamoyltransferase catalytic subunit -
  OZ428_RS31215 (OZ428_31215) - 6595258..6596529 (+) 1272 WP_269132232.1 dihydroorotase -
  OZ428_RS31220 (OZ428_31220) - 6596575..6596985 (-) 411 WP_269134239.1 TM2 domain-containing protein -
  OZ428_RS31225 (OZ428_31225) - 6597254..6597895 (+) 642 WP_028618303.1 C40 family peptidase -
  OZ428_RS31230 (OZ428_31230) pilT 6598150..6599184 (-) 1035 WP_269132233.1 type IV pilus twitching motility protein PilT Machinery gene
  OZ428_RS31235 (OZ428_31235) - 6599242..6599928 (+) 687 WP_133075462.1 YggS family pyridoxal phosphate-dependent enzyme -
  OZ428_RS31240 (OZ428_31240) proC 6599962..6600780 (+) 819 WP_269132234.1 pyrroline-5-carboxylate reductase -
  OZ428_RS31245 (OZ428_31245) - 6600790..6601380 (+) 591 WP_033897055.1 YggT family protein -
  OZ428_RS31250 (OZ428_31250) - 6601476..6602615 (+) 1140 WP_095013877.1 homoserine O-acetyltransferase -
  OZ428_RS31255 (OZ428_31255) metW 6602623..6603243 (+) 621 WP_010207236.1 methionine biosynthesis protein MetW -
  OZ428_RS31260 (OZ428_31260) - 6603265..6603699 (+) 435 WP_016976033.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37309.06 Da        Isoelectric Point: 6.8430

>NTDB_id=764444 OZ428_RS31230 WP_269132233.1 6598150..6599184(-) (pilT) [Pseudomonas sp. GXZC]
MDITELLTASVRRGASDLHVSAGLAPMLRLDGEVWPLEGPVLSPTQVADLLSPLLTQHQQKDFETSLETDFAFELPGVAR
FRANVFRQDRGMSAVFRTIPSEVQSLESLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDFLNQHRRQHILTLED
PIEFIHTPKMALINQRQVHRDTHGFSTALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGAGVISREDAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=764444 OZ428_RS31230 WP_269132233.1 6598150..6599184(-) (pilT) [Pseudomonas sp. GXZC]
ATGGATATCACTGAATTACTCACGGCCAGCGTGCGCCGTGGTGCCTCCGACCTGCATGTGTCTGCCGGCCTGGCGCCGAT
GCTGCGACTGGATGGCGAGGTCTGGCCGCTGGAGGGGCCGGTGCTTTCACCTACGCAAGTAGCGGACTTATTGAGTCCTT
TGCTCACTCAGCACCAACAAAAGGATTTCGAAACATCTCTTGAAACAGACTTTGCCTTCGAACTGCCCGGCGTGGCGCGC
TTCCGGGCCAATGTGTTCCGGCAGGATCGCGGCATGAGTGCGGTGTTTCGCACCATCCCTTCTGAGGTCCAGAGCCTGGA
AAGCCTCGGTCTTGGCGAAGTGTTCCAGCGTATCGCCCAACTGCCACGTGGTCTGGTGCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCTACGACTCTGGCGGCGATGATCGATTTTCTCAATCAGCATCGGCGTCAGCACATCCTCACCCTTGAAGAC
CCCATCGAATTTATCCACACGCCGAAAATGGCGTTGATCAACCAGCGCCAGGTGCATCGCGATACCCATGGTTTTTCCAC
TGCCTTGCGCTCGGCACTGCGCGAAGACCCGGACGTGATCCTGGTGGGCGAACTGCGCGACCTGGAAACCATCCGCCTCG
CGCTGACGGCGGCTGAGACCGGGCACCTAGTGTTTGGCACCCTGCACACCAGTTCGGCGGCAAAGACTGTCGACCGGCTG
GTGGACGTGTTCCCGGCCGGGGAAAAGGCCATGGTCCGCTCGATGCTGTCGGAGTCGTTGCAGGCGGTGGTGTCTCAGGT
ACTGGTGAAGAAAGTCGGCGGCGGACGCGTGGCGGCTCATGAAATCATGCTGGGCACGCCGGCCATTCGGAATTTGATCC
GGGAGGACAAGGTGGCGCAGATGGTTTCAGCGATACAGACGGGTGGGGCGTTGGGGATGAAGACGCTGGACATGAGTTTG
AAGGCGTTGGTCGGCGCGGGGGTGATCAGCCGGGAGGATGCGAGGGAGAAGGCGAGGGTGCCAGCAGACATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.674

100

0.727
  pilT Pseudomonas stutzeri DSM 10701

70.93

100

0.709
  pilT Acinetobacter nosocomialis M2

69.501

99.128

0.689
  pilT Acinetobacter baumannii D1279779

69.501

99.128

0.689
  pilT Acinetobacter baumannii strain A118

69.501

99.128

0.689
  pilT Acinetobacter baylyi ADP1

68.622

99.128

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.392

97.965

0.631
  pilT Vibrio cholerae strain A1552

64.392

97.965

0.631
  pilT Legionella pneumophila strain Lp02

63.609

98.256

0.625
  pilT Legionella pneumophila strain ERS1305867

63.609

98.256

0.625
  pilT Neisseria meningitidis 8013

61.176

98.837

0.605
  pilT Neisseria gonorrhoeae MS11

60.882

98.837

0.602
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.406

100

0.485
  pilU Vibrio cholerae strain A1552

38.841

100

0.39
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387
  pilU Pseudomonas stutzeri DSM 10701

38.806

97.384

0.378