Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   O1V65_RS22025 Genome accession   NZ_CP114062
Coordinates   4762418..4763407 (+) Length   329 a.a.
NCBI ID   WP_269130537.1    Uniprot ID   -
Organism   Rouxiella badensis strain DAR84756     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4753087..4762282 4762418..4763407 flank 136


Gene organization within MGE regions


Location: 4753087..4763407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1V65_RS21980 (O1V65_21975) - 4753087..4754526 (+) 1440 WP_269130032.1 type III restriction endonuclease subunit R -
  O1V65_RS21985 (O1V65_21980) - 4755615..4756751 (-) 1137 WP_269130033.1 DUF3800 domain-containing protein -
  O1V65_RS21990 (O1V65_21985) - 4757062..4757325 (+) 264 WP_269130034.1 hypothetical protein -
  O1V65_RS21995 (O1V65_21990) - 4757879..4758529 (+) 651 WP_269130035.1 hypothetical protein -
  O1V65_RS22000 (O1V65_21995) - 4759126..4759740 (-) 615 WP_269130036.1 LexA family transcriptional regulator -
  O1V65_RS22005 (O1V65_22000) - 4759841..4760098 (+) 258 WP_269130037.1 helix-turn-helix domain-containing protein -
  O1V65_RS22010 (O1V65_22005) - 4760104..4760910 (+) 807 WP_269130038.1 hypothetical protein -
  O1V65_RS22015 (O1V65_22010) - 4760922..4761278 (+) 357 WP_269130039.1 hypothetical protein -
  O1V65_RS22020 (O1V65_22015) - 4761707..4762282 (+) 576 WP_269130040.1 recombinase family protein -
  O1V65_RS22025 (O1V65_22020) pilT 4762418..4763407 (+) 990 WP_269130537.1 type IV pilus twitching motility protein PilT Machinery gene

Sequence

Protein


Download         Length: 329 a.a.        Molecular weight: 35841.97 Da        Isoelectric Point: 7.0149

>NTDB_id=764312 O1V65_RS22025 WP_269130537.1 4762418..4763407(+) (pilT) [Rouxiella badensis strain DAR84756]
MQNSSVGNFLKASDLHLCTGQLPMLRIDGELIGGQYSERLTPAQMVALSEEWLGDAQQIELKQLGQADAAVTLASGRRIR
VNFFRQFSGLSAALRPLPQRCPSLADLNAPDILRRLSEHEDGLILITGATGSGKSTTLAAMIGHLNQTRTRHIITLEDPI
EFLHTSDRCLIQQRELGTHTPSFVQAIRAALREDPDVLLLGELRDSDSIRLALTAAETGHLVLATLHARHAAQAVDRLID
VFPADEKAFVRAQLAGSLRGILAQKLLTAKQGGRVALYEMLTSTPAVRNLIREGKTHQLQDSLQTGGEAGMQTFEQCRQQ
RVSAGLLAD

Nucleotide


Download         Length: 990 bp        

>NTDB_id=764312 O1V65_RS22025 WP_269130537.1 4762418..4763407(+) (pilT) [Rouxiella badensis strain DAR84756]
ATCCAAAATAGTAGTGTAGGTAACTTCCTTAAAGCCTCGGATCTGCACCTTTGTACCGGTCAGTTGCCTATGTTACGCAT
TGACGGAGAACTGATCGGCGGCCAATATTCCGAACGTCTCACCCCTGCGCAGATGGTGGCGCTCAGTGAGGAGTGGCTCG
GCGACGCCCAGCAAATTGAGTTAAAACAGCTTGGGCAGGCCGACGCGGCGGTTACGCTGGCAAGCGGCAGGCGTATCAGG
GTTAACTTCTTTCGCCAATTCTCCGGGCTCTCGGCGGCGCTGCGACCGCTGCCGCAGCGTTGCCCGAGTCTTGCAGACTT
GAATGCCCCCGATATTCTGCGCCGGCTCAGTGAACACGAAGATGGCCTGATTCTGATTACCGGTGCCACCGGCAGCGGTA
AATCGACCACGCTGGCGGCGATGATTGGCCACCTCAATCAGACCCGCACACGGCACATTATCACCTTGGAAGACCCGATT
GAATTCTTGCACACCAGCGACCGTTGTTTAATTCAACAGCGTGAGCTGGGCACTCATACGCCTTCATTTGTACAGGCCAT
ACGCGCTGCGCTGCGAGAGGATCCTGACGTACTGCTGCTCGGCGAACTTCGCGACAGTGACAGTATTCGACTGGCGTTAA
CCGCCGCCGAAACGGGGCATCTGGTGCTGGCCACGCTTCATGCTCGCCATGCCGCACAGGCCGTTGACCGGCTAATTGAC
GTCTTTCCCGCCGACGAAAAGGCCTTCGTCCGTGCACAGCTTGCCGGAAGCCTGCGTGGCATATTGGCGCAAAAACTGCT
GACCGCTAAGCAGGGCGGCAGGGTTGCGTTATATGAGATGCTGACCTCGACACCGGCGGTCCGTAATTTGATTCGTGAGG
GAAAGACGCATCAGTTGCAGGACAGCCTGCAAACCGGCGGTGAAGCCGGCATGCAGACCTTTGAGCAGTGCAGGCAGCAG
CGCGTGAGCGCGGGGTTGCTTGCAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.157

96.96

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.157

96.96

0.486
  pilT Neisseria meningitidis 8013

49.216

96.96

0.477
  pilT Neisseria gonorrhoeae MS11

48.903

96.96

0.474
  pilT Legionella pneumophila strain Lp02

46.177

99.392

0.459
  pilT Legionella pneumophila strain ERS1305867

46.177

99.392

0.459
  pilT Pseudomonas stutzeri DSM 10701

46.894

97.872

0.459
  pilT Pseudomonas aeruginosa PAK

47.003

96.353

0.453
  pilT Acinetobacter baylyi ADP1

46.273

97.872

0.453
  pilT Acinetobacter nosocomialis M2

45.963

97.872

0.45
  pilT Acinetobacter baumannii D1279779

45.652

97.872

0.447
  pilT Acinetobacter baumannii strain A118

45.652

97.872

0.447
  pilU Vibrio cholerae strain A1552

42.994

95.441

0.41
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.951

92.705

0.398
  pilU Pseudomonas stutzeri DSM 10701

39.088

93.313

0.365
  pilU Acinetobacter baylyi ADP1

38.835

93.921

0.365