Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   O0N60_RS39540 Genome accession   NZ_CP114039
Coordinates   9718738..9719850 (-) Length   370 a.a.
NCBI ID   WP_206789813.1    Uniprot ID   -
Organism   Corallococcus sp. NCRR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 9713738..9724850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O0N60_RS39525 (O0N60_39525) - 9714396..9715763 (-) 1368 WP_242544336.1 sigma-54 dependent transcriptional regulator -
  O0N60_RS39530 (O0N60_39530) - 9715829..9717397 (-) 1569 WP_269012797.1 ATP-binding protein -
  O0N60_RS39535 (O0N60_39535) - 9717426..9718682 (-) 1257 WP_206789815.1 type II secretion system F family protein -
  O0N60_RS39540 (O0N60_39540) pilT 9718738..9719850 (-) 1113 WP_206789813.1 type IV pilus twitching motility protein PilT Machinery gene
  O0N60_RS39545 (O0N60_39545) pilB 9719896..9721596 (-) 1701 WP_120589090.1 type IV-A pilus assembly ATPase PilB -
  O0N60_RS39550 (O0N60_39550) - 9721819..9722745 (-) 927 WP_206789811.1 bifunctional riboflavin kinase/FAD synthetase -
  O0N60_RS39555 (O0N60_39555) - 9722827..9723447 (+) 621 WP_206789809.1 tRNA (guanine-N7)-methyltransferase -
  O0N60_RS39560 (O0N60_39560) - 9723622..9724665 (+) 1044 WP_206789807.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40426.59 Da        Isoelectric Point: 8.2875

>NTDB_id=764229 O0N60_RS39540 WP_206789813.1 9718738..9719850(-) (pilT) [Corallococcus sp. NCRR]
MANLHQLLKAMVEKGSSDLHITTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLS
RFRANIFMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQLSFVLEGVMSQALVSKVGGPGRVLALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKYGMQTFN
QALAALLARRVISQDEAFGRSSDPEELRNILATGGGTGGAQRPAGGAGAR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=764229 O0N60_RS39540 WP_206789813.1 9718738..9719850(-) (pilT) [Corallococcus sp. NCRR]
GTGGCCAACCTGCACCAGCTTCTCAAGGCGATGGTCGAGAAGGGCTCTTCCGACCTCCACATCACCACCGGCTCGCCGCC
GCAGCTCCGCGTGGACGGTGAGCTCGTCCCGCTCAAGACGGCGCCGCTCACGCCCGTGGAGACCAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAACTGGACCTGTCGTTCGGCGTGAAGGGCCTGTCA
CGCTTCCGCGCGAACATCTTCATGCAGCGCGGCGCCGTCGCCGGTGCGTTCCGCACGATTCCGTTCAAGATTCTGACGTT
CCAGGAGCTGGGCCTGCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGCCGCGCGGCCTCATCCTGGTGACGGGCCCCACGG
GCTCCGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTGGTCAACCAGCGCGAGGTCGGTGCGGACACGCGCAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGATCCGGACGTGGTGCTCGTCGGCGAGCTCCGAGACCTGGAGACCATCGAAG
CGGCGCTCACCATCGCGGAGACGGGCCACATCTGCTACGCGACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTCTTTCCCCCGTACCAGCAGCCCCAGGTGCGCGCCCAGCTGTCCTTCGTGCTGGAGGGCGTGATGAGCCA
GGCGCTCGTCTCCAAGGTGGGCGGGCCGGGCCGCGTGCTGGCCCTGGAGGTCATGGTGCCCAACCCCGCCATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCGTCCATGCAGGTGGGCCAGGCGAAGTACGGCATGCAGACGTTCAAC
CAGGCGCTGGCCGCCCTTCTGGCGCGCCGGGTCATCTCCCAGGACGAGGCCTTCGGCCGTTCCTCCGACCCGGAGGAGCT
GCGCAACATCCTCGCCACGGGTGGCGGGACGGGCGGAGCCCAGCGGCCGGCCGGGGGAGCGGGTGCCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

98.378

0.492
  pilT Legionella pneumophila strain Lp02

54.03

90.541

0.489
  pilT Legionella pneumophila strain ERS1305867

54.03

90.541

0.489
  pilT Pseudomonas stutzeri DSM 10701

51.884

93.243

0.484
  pilT Pseudomonas aeruginosa PAK

51.594

93.243

0.481
  pilT Acinetobacter baumannii D1279779

51.594

93.243

0.481
  pilT Acinetobacter baumannii strain A118

51.594

93.243

0.481
  pilT Acinetobacter nosocomialis M2

51.304

93.243

0.478
  pilT Acinetobacter baylyi ADP1

51.014

93.243

0.476
  pilT Vibrio cholerae strain A1552

49.401

90.27

0.446
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.401

90.27

0.446
  pilT Neisseria meningitidis 8013

44.575

92.162

0.411
  pilT Neisseria gonorrhoeae MS11

44.575

92.162

0.411
  pilU Vibrio cholerae strain A1552

43.059

95.405

0.411
  pilU Pseudomonas stutzeri DSM 10701

41.292

96.216

0.397
  pilU Acinetobacter baylyi ADP1

40.346

93.784

0.378