Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DSM112329_RS15610 Genome accession   NZ_CP114014
Coordinates   3204795..3205907 (-) Length   370 a.a.
NCBI ID   WP_354697479.1    Uniprot ID   A0AAU7AX81
Organism   Paraconexibacter sp. AEG42_29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3199795..3210907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSM112329_RS15590 (DSM112329_03105) - 3201983..3202399 (+) 417 WP_354697475.1 hypothetical protein -
  DSM112329_RS15595 (DSM112329_03106) - 3202409..3202954 (+) 546 WP_354697476.1 RES family NAD+ phosphorylase -
  DSM112329_RS15600 (DSM112329_03107) - 3202956..3203528 (-) 573 WP_354697477.1 hypothetical protein -
  DSM112329_RS15605 (DSM112329_03108) - 3203584..3204792 (-) 1209 WP_354697478.1 type II secretion system F family protein -
  DSM112329_RS15610 (DSM112329_03109) pilT 3204795..3205907 (-) 1113 WP_354697479.1 type IV pilus twitching motility protein PilT Machinery gene
  DSM112329_RS15615 (DSM112329_03110) - 3205978..3207780 (-) 1803 WP_354697480.1 ATPase, T2SS/T4P/T4SS family -
  DSM112329_RS15620 (DSM112329_03111) aroE 3207782..3208588 (-) 807 WP_354697481.1 shikimate dehydrogenase -
  DSM112329_RS15625 (DSM112329_03112) mltG 3208588..3210489 (-) 1902 WP_354697482.1 endolytic transglycosylase MltG -
  DSM112329_RS15630 (DSM112329_03113) ruvX 3210486..3210890 (-) 405 WP_354697483.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40059.72 Da        Isoelectric Point: 6.4296

>NTDB_id=764040 DSM112329_RS15610 WP_354697479.1 3204795..3205907(-) (pilT) [Paraconexibacter sp. AEG42_29]
MRFDFADLLLDVLDRRASDLHITAGSHPMVRVRGRLTPLDEYPMLDATETREIVYSILSNDQRQRLETEWQLDFAYSIPG
RARFRVNAYFQRGALGAAFRLIPAQITPIDDLGLPPVVHELCKKPRGIVLVTGPTGSGKSTTLASMVDEINATREEHILT
IEDPIEFLHGHRKCMVNQREIGADAHSFAAGLKAALREDPDVILVGEMRDLDTISTALTAAETGHLVFATLHTQSAPSTI
DRVIDVFPAHQQQQIRVQLSIALQGVITQTLIPTADGAGRAVACEILVPTAAVRNLIREGKTHQILSSMQTGSGVGMQSM
DTALAALVRSGKITQRVAEARSDTPDELRRILGASSFGGGGAGGNYGMAA

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=764040 DSM112329_RS15610 WP_354697479.1 3204795..3205907(-) (pilT) [Paraconexibacter sp. AEG42_29]
ATGCGCTTCGATTTCGCCGATCTCCTCCTTGACGTCCTGGACCGCCGGGCGTCCGACCTCCACATCACCGCCGGCTCGCA
TCCCATGGTGCGCGTGCGGGGCCGGCTGACGCCGCTGGACGAGTACCCGATGCTCGACGCGACCGAGACGCGGGAGATCG
TCTACTCGATCCTCTCGAACGACCAGCGCCAGCGCCTGGAGACCGAGTGGCAGCTCGACTTCGCCTACTCGATCCCGGGC
CGCGCCCGCTTCCGCGTCAACGCCTACTTCCAGCGCGGTGCCCTCGGTGCCGCCTTCCGCCTGATCCCGGCGCAGATCAC
GCCGATCGACGACCTCGGCCTGCCGCCGGTCGTCCACGAGCTGTGCAAGAAGCCGCGCGGCATCGTCCTCGTGACGGGCC
CGACCGGCTCCGGCAAGTCCACGACGCTCGCGTCCATGGTCGACGAGATCAACGCGACCCGCGAGGAGCACATCCTCACG
ATCGAGGACCCGATCGAGTTCCTGCACGGCCACCGCAAGTGCATGGTCAACCAGCGGGAGATCGGGGCCGACGCGCACTC
GTTCGCCGCCGGCCTGAAGGCCGCCCTACGTGAGGATCCGGACGTCATCCTCGTCGGCGAGATGCGCGACCTGGACACCA
TCTCCACCGCGCTCACCGCGGCGGAGACCGGCCACCTCGTGTTCGCGACGCTGCACACGCAGAGCGCGCCGTCCACCATC
GACCGCGTCATCGACGTCTTCCCCGCCCATCAGCAGCAGCAGATCCGGGTCCAGCTCTCGATCGCGCTGCAGGGCGTGAT
CACGCAGACGCTGATCCCGACCGCCGACGGCGCCGGCCGCGCGGTCGCCTGCGAGATCCTCGTCCCGACCGCCGCCGTGC
GGAACCTGATCCGGGAGGGCAAGACCCACCAGATCCTCTCCTCGATGCAGACCGGCTCCGGGGTCGGGATGCAGTCGATG
GACACCGCCCTGGCCGCGCTCGTGCGCTCGGGCAAGATCACCCAGCGCGTCGCCGAGGCGCGGTCGGACACGCCGGACGA
GCTGCGCCGGATCCTCGGCGCGTCGAGCTTCGGCGGCGGCGGAGCCGGTGGCAACTACGGGATGGCGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.5

97.297

0.511
  pilT Acinetobacter baumannii D1279779

51.156

93.514

0.478
  pilT Acinetobacter baumannii strain A118

51.156

93.514

0.478
  pilT Acinetobacter nosocomialis M2

51.156

93.514

0.478
  pilT Pseudomonas aeruginosa PAK

50.867

93.514

0.476
  pilT Acinetobacter baylyi ADP1

50.867

93.514

0.476
  pilT Legionella pneumophila strain ERS1305867

52.537

90.541

0.476
  pilT Legionella pneumophila strain Lp02

52.537

90.541

0.476
  pilT Pseudomonas stutzeri DSM 10701

50.578

93.514

0.473
  pilT Neisseria meningitidis 8013

49.708

92.432

0.459
  pilT Neisseria gonorrhoeae MS11

49.415

92.432

0.457
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.788

91.622

0.438
  pilT Vibrio cholerae strain A1552

47.788

91.622

0.438
  pilU Acinetobacter baylyi ADP1

43.228

93.784

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.525

95.676

0.397
  pilU Vibrio cholerae strain A1552

39.205

95.135

0.373