Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DSM104299_RS16390 Genome accession   NZ_CP114013
Coordinates   3239470..3240555 (-) Length   361 a.a.
NCBI ID   WP_272472712.1    Uniprot ID   -
Organism   Baekduia alba strain 0141_2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3234470..3245555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSM104299_RS16365 (DSM104299_03281) - 3234586..3235743 (+) 1158 WP_272472707.1 HRDC domain-containing protein -
  DSM104299_RS16370 (DSM104299_03282) - 3235753..3236856 (-) 1104 WP_272472708.1 GGDEF domain-containing protein -
  DSM104299_RS16375 (DSM104299_03283) - 3236960..3237535 (-) 576 WP_272472709.1 hypothetical protein -
  DSM104299_RS16380 (DSM104299_03284) - 3237592..3238173 (+) 582 WP_272472710.1 DoxX family protein -
  DSM104299_RS16385 (DSM104299_03285) - 3238243..3239451 (-) 1209 WP_272472711.1 type II secretion system F family protein -
  DSM104299_RS16390 (DSM104299_03286) pilT 3239470..3240555 (-) 1086 WP_272472712.1 type IV pilus twitching motility protein PilT Machinery gene
  DSM104299_RS16395 (DSM104299_03287) - 3240619..3241578 (-) 960 WP_272472713.1 aldo/keto reductase -
  DSM104299_RS16400 (DSM104299_03288) - 3241936..3242076 (-) 141 WP_272472714.1 hypothetical protein -
  DSM104299_RS16405 (DSM104299_03289) - 3242229..3242891 (+) 663 WP_272472715.1 GNAT family N-acetyltransferase -
  DSM104299_RS16410 (DSM104299_03290) - 3242888..3243865 (-) 978 WP_272472716.1 hypothetical protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39404.11 Da        Isoelectric Point: 6.6640

>NTDB_id=764029 DSM104299_RS16390 WP_272472712.1 3239470..3240555(-) (pilT) [Baekduia alba strain 0141_2]
MRFDFAQLLLDVLERRASDLHITAGSHPMIRVRGRLTSLEDYPVLDPTDTREIIYGILSNDQRQRLETDWQLDFAYAVPG
TARFRVNAYFQRSAMSAAFRLIPSVITKIDGLGLPPVVHEFCKKPRGLVLVTGPTGSGKSTSLAAMIDEINETREEHILT
IEDPIEFLHSHKKCMVNQRELGSDAQGFGPALKGALRQDPDVILVGEMRDLETISTALTAAETGHLVFATLHTQSAPQTI
DRIIDVFPSHQQDQVRTQLSVALQGVVTQTLIPTADGSARVVAAETLVPTPAVRNLIREGKTHQILSAMQTGGGTGMQTL
DMALASLIRAGKITQRVAESRSSTPEELKRLSMGLGGLQAA

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=764029 DSM104299_RS16390 WP_272472712.1 3239470..3240555(-) (pilT) [Baekduia alba strain 0141_2]
ATGCGGTTCGATTTCGCCCAGCTCCTGCTCGATGTCCTCGAGCGTCGTGCCTCCGACCTGCACATCACGGCCGGATCGCA
CCCGATGATCCGCGTCCGTGGTCGCCTGACGTCGCTGGAGGACTATCCGGTCCTCGACCCGACGGACACCCGCGAGATCA
TCTACGGGATCCTCAGCAACGACCAGCGCCAGCGCCTGGAGACGGACTGGCAGCTGGACTTCGCCTACGCGGTCCCGGGC
ACCGCCCGCTTCCGTGTCAACGCGTACTTCCAGCGGTCGGCGATGAGCGCGGCGTTCCGCCTCATCCCGTCGGTCATCAC
CAAGATCGACGGCCTCGGCCTGCCGCCCGTCGTCCACGAGTTCTGCAAGAAGCCGCGTGGCCTGGTCCTCGTGACCGGTC
CGACCGGCTCCGGCAAGTCCACCTCGCTCGCCGCGATGATCGACGAGATCAACGAGACGCGCGAGGAGCACATCCTCACG
ATCGAGGACCCGATCGAGTTCCTGCACTCCCACAAGAAGTGCATGGTCAACCAGCGTGAGCTGGGCTCCGACGCGCAGGG
CTTCGGCCCGGCGCTGAAGGGCGCGCTGCGCCAGGACCCCGACGTGATCCTCGTCGGCGAGATGCGCGACCTGGAGACCA
TCTCGACCGCCCTGACGGCGGCTGAGACCGGCCACCTGGTCTTCGCCACCCTGCACACCCAGAGCGCGCCGCAGACCATC
GACCGCATCATCGACGTGTTCCCGAGCCACCAGCAGGACCAGGTCCGGACGCAGCTGTCGGTCGCGCTCCAGGGCGTGGT
CACCCAGACGCTGATCCCGACCGCCGACGGCTCGGCGCGCGTCGTGGCGGCAGAGACGCTCGTCCCGACCCCCGCGGTCC
GCAACCTCATCCGCGAGGGCAAGACCCATCAGATCCTGTCGGCGATGCAGACCGGCGGCGGCACCGGCATGCAGACGCTC
GACATGGCGCTCGCCTCGCTCATCCGCGCCGGCAAGATCACGCAGCGCGTCGCCGAGTCGCGCTCCTCCACGCCCGAGGA
GCTCAAGCGCCTGTCCATGGGCCTGGGCGGCCTCCAGGCCGCATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.089

99.446

0.518
  pilT Pseudomonas aeruginosa PAK

53.757

95.845

0.515
  pilT Acinetobacter baylyi ADP1

53.179

95.845

0.51
  pilT Pseudomonas stutzeri DSM 10701

52.601

95.845

0.504
  pilT Acinetobacter nosocomialis M2

52.601

95.845

0.504
  pilT Acinetobacter baumannii D1279779

52.601

95.845

0.504
  pilT Acinetobacter baumannii strain A118

52.601

95.845

0.504
  pilT Legionella pneumophila strain ERS1305867

54.03

92.798

0.501
  pilT Legionella pneumophila strain Lp02

54.03

92.798

0.501
  pilT Neisseria meningitidis 8013

50.448

92.798

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.558

93.906

0.465
  pilT Vibrio cholerae strain A1552

49.558

93.906

0.465
  pilT Neisseria gonorrhoeae MS11

50.149

92.798

0.465
  pilU Pseudomonas stutzeri DSM 10701

43.503

98.061

0.427
  pilU Acinetobacter baylyi ADP1

42.09

98.061

0.413
  pilU Vibrio cholerae strain A1552

39.769

96.122

0.382