Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OTK01_RS04330 Genome accession   NZ_CP113866
Coordinates   911326..912381 (+) Length   351 a.a.
NCBI ID   WP_269011786.1    Uniprot ID   -
Organism   Caldicellulosiruptor acetigenus strain DSM 7040     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 906326..917381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTK01_RS04320 (OTK01_000864) - 906424..909510 (+) 3087 WP_029227964.1 response regulator -
  OTK01_RS04325 (OTK01_000865) - 909561..911258 (+) 1698 WP_029227963.1 GspE/PulE family protein -
  OTK01_RS04330 (OTK01_000866) pilT 911326..912381 (+) 1056 WP_269011786.1 type IV pilus twitching motility protein PilT Machinery gene
  OTK01_RS04335 (OTK01_000867) - 912400..913611 (+) 1212 WP_029227961.1 type II secretion system F family protein -
  OTK01_RS04340 (OTK01_000868) - 913627..914049 (+) 423 WP_029227960.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OTK01_RS04345 (OTK01_000869) - 914074..914508 (+) 435 WP_029227959.1 type II secretion system protein -
  OTK01_RS04350 (OTK01_000870) - 914515..915279 (+) 765 WP_051129948.1 prepilin peptidase -
  OTK01_RS04355 (OTK01_000871) - 915318..915584 (+) 267 WP_029227957.1 late competence development ComFB family protein -
  OTK01_RS13225 - 915614..916216 (+) 603 WP_029227956.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OTK01_RS04365 (OTK01_000873) - 916213..916698 (+) 486 WP_029227955.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39404.51 Da        Isoelectric Point: 9.0617

>NTDB_id=763322 OTK01_RS04330 WP_269011786.1 911326..912381(+) (pilT) [Caldicellulosiruptor acetigenus strain DSM 7040]
MDINEILKEAFLKGASDIHITAGSPPIMRVHGVLTRMSDLSLTPEITERFVKEITNEYQYKRLQEAGEVDFSYSIRGLSR
FRVNAFRQRGSYAIAFRVISQDIPKFETLGLPPVLKDFTKLNKGLVLVTGPTGSGKSTTLASLIDIINAERNLHIITLED
PIEYLHRHKKSIVNQREIGNDTQSFAAALRAALREDPDVILVGEMRDLETISIALTAAETGHLVFSTLHTVGAAKTIDRI
IDVFPPYQQQQIRVQLSTVLQAVVSQQLLTRRDGRGRVVAVEVMIANAAIRNLIREAKTYQIQSIIQTHTKNGMITMEQS
LVDLYKRGLITREDAIMYASDHELINKLLIF

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=763322 OTK01_RS04330 WP_269011786.1 911326..912381(+) (pilT) [Caldicellulosiruptor acetigenus strain DSM 7040]
ATGGATATCAATGAGATTTTGAAAGAGGCATTTTTGAAAGGCGCATCTGATATACATATCACGGCAGGTTCACCCCCTAT
CATGAGGGTGCACGGTGTACTTACACGCATGAGCGATTTGAGCCTCACACCAGAGATAACTGAAAGGTTTGTAAAAGAAA
TTACAAACGAATACCAGTACAAACGTTTGCAAGAAGCTGGTGAGGTTGACTTTTCTTACAGCATTAGAGGTTTGAGCAGG
TTTAGGGTAAATGCATTTAGGCAAAGAGGTTCTTATGCTATTGCATTCAGAGTCATATCTCAGGATATACCAAAGTTTGA
GACATTGGGACTTCCTCCGGTTCTGAAAGATTTTACAAAGCTCAACAAAGGGCTTGTTCTTGTTACTGGTCCAACCGGTT
CAGGGAAATCGACCACGTTAGCATCGCTTATAGATATAATAAATGCTGAGAGGAACTTACATATTATCACATTGGAAGAC
CCTATTGAATATCTTCACAGGCACAAAAAGAGTATTGTCAATCAAAGAGAGATAGGAAACGACACACAAAGCTTTGCAGC
AGCCCTGAGAGCTGCTCTTCGTGAAGACCCTGATGTAATTCTGGTTGGCGAGATGAGAGATTTAGAGACAATTTCCATAG
CACTTACCGCTGCAGAGACCGGACACTTGGTTTTTTCAACACTTCACACTGTTGGTGCAGCAAAGACCATTGACAGAATC
ATCGATGTTTTCCCACCATATCAGCAGCAGCAAATACGTGTTCAGCTTTCTACAGTTCTTCAAGCAGTTGTATCGCAACA
GCTTTTGACAAGGAGAGACGGCAGGGGCAGGGTTGTTGCGGTTGAGGTTATGATAGCAAACGCGGCAATAAGGAATCTCA
TAAGAGAAGCAAAGACGTATCAGATACAATCAATTATTCAAACACATACGAAAAATGGAATGATAACAATGGAACAATCT
CTTGTTGACTTGTACAAAAGAGGGCTTATAACAAGAGAAGATGCAATAATGTATGCATCTGATCATGAGCTTATAAACAA
GCTTTTGATATTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.299

99.145

0.519
  pilT Legionella pneumophila strain Lp02

53.731

95.442

0.513
  pilT Legionella pneumophila strain ERS1305867

53.731

95.442

0.513
  pilT Acinetobacter nosocomialis M2

53.433

95.442

0.51
  pilT Acinetobacter baumannii D1279779

53.433

95.442

0.51
  pilT Acinetobacter baumannii strain A118

53.433

95.442

0.51
  pilT Neisseria meningitidis 8013

51.744

98.006

0.507
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.134

95.442

0.507
  pilT Vibrio cholerae strain A1552

53.134

95.442

0.507
  pilT Pseudomonas stutzeri DSM 10701

52.679

95.726

0.504
  pilT Pseudomonas aeruginosa PAK

52.239

95.442

0.499
  pilT Neisseria gonorrhoeae MS11

51.02

97.721

0.499
  pilT Acinetobacter baylyi ADP1

52.568

94.302

0.496
  pilU Pseudomonas stutzeri DSM 10701

43.658

96.581

0.422
  pilU Acinetobacter baylyi ADP1

41.765

96.866

0.405
  pilU Vibrio cholerae strain A1552

38.643

96.581

0.373