Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OTK00_RS08415 Genome accession   NZ_CP113865
Coordinates   1688013..1689071 (-) Length   352 a.a.
NCBI ID   WP_045169850.1    Uniprot ID   -
Organism   Caldicellulosiruptor morganii strain DSM 8990     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1683013..1694071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTK00_RS08380 (OTK00_001676) - 1683454..1683987 (-) 534 WP_045169847.1 PilW family protein -
  OTK00_RS08385 (OTK00_001677) - 1683984..1684535 (-) 552 WP_045170211.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OTK00_RS08390 (OTK00_001678) - 1684556..1684849 (-) 294 WP_045170212.1 late competence development ComFB family protein -
  OTK00_RS08395 (OTK00_001679) - 1684860..1685645 (-) 786 WP_045170213.1 A24 family peptidase -
  OTK00_RS08400 (OTK00_001680) - 1685784..1686194 (-) 411 WP_045170214.1 hypothetical protein -
  OTK00_RS08405 (OTK00_001681) - 1686273..1686695 (-) 423 WP_045169848.1 type II secretion system protein -
  OTK00_RS08410 (OTK00_001682) - 1686749..1687963 (-) 1215 WP_045169849.1 type II secretion system F family protein -
  OTK00_RS08415 (OTK00_001683) pilT 1688013..1689071 (-) 1059 WP_045169850.1 type IV pilus twitching motility protein PilT Machinery gene
  OTK00_RS08420 (OTK00_001684) - 1689108..1690823 (-) 1716 WP_045169851.1 GspE/PulE family protein -
  OTK00_RS08425 (OTK00_001685) - 1690823..1693858 (-) 3036 WP_082054641.1 response regulator -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39532.50 Da        Isoelectric Point: 6.7296

>NTDB_id=763315 OTK00_RS08415 WP_045169850.1 1688013..1689071(-) (pilT) [Caldicellulosiruptor morganii strain DSM 8990]
MHINEILTEAFLKNASDIHITPGVPPVFRIHGKLVKMEDEDNLTAEMVEEYVRQILTPEQFSKLEEVGELDFSYSIRGVS
RFRVNAYKQRGSYSIAFRVITANIPAFESLGLPAVLKEFTKLNKGLVLVTGPTGSGKSTTLASLIDIMNSERDLHIITLE
DPIEYLHKHKKSIINQREIGNDTKSFANALRAALREDPDVILVGEMRDLETISIALTAAETGHVVFSTLHTVGAAKTIDR
IIDVFPPHQQQQIRVQLSTVLQGVVSQQLLTRRDGKGRVVATEVMICNPAIRNLIRENKTYQIPSIIQTHQRVGMITMEQ
SLIELYKRGLITRDDAYAHATDFDYMEKLLSA

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=763315 OTK00_RS08415 WP_045169850.1 1688013..1689071(-) (pilT) [Caldicellulosiruptor morganii strain DSM 8990]
ATGCATATAAATGAGATTCTGACAGAAGCATTTCTAAAAAACGCGTCTGACATTCATATTACACCGGGGGTGCCACCTGT
TTTTCGAATACACGGCAAACTTGTGAAAATGGAAGACGAAGATAATCTCACCGCCGAGATGGTTGAGGAATATGTGCGAC
AGATTCTGACTCCCGAGCAATTCAGCAAACTTGAAGAGGTGGGTGAGCTGGACTTTTCTTACAGTATAAGAGGGGTAAGC
AGATTCAGAGTAAATGCTTACAAGCAACGTGGTTCATATTCAATAGCTTTCAGAGTGATTACCGCCAATATACCAGCTTT
TGAAAGTCTGGGTCTTCCTGCTGTTTTAAAAGAATTTACAAAATTAAATAAAGGGCTTGTACTGGTAACAGGTCCCACGG
GCTCGGGTAAATCAACAACTCTGGCTTCGTTAATTGATATAATGAATAGTGAAAGAGATTTACATATTATAACACTGGAA
GATCCTATTGAGTATTTGCATAAACATAAAAAAAGTATAATCAATCAGAGGGAAATAGGAAATGATACAAAAAGCTTTGC
TAATGCTCTGAGAGCAGCACTGCGTGAAGATCCAGATGTTATCCTGGTTGGTGAGATGAGAGATCTGGAAACCATCTCTA
TTGCGCTAACAGCTGCTGAGACCGGTCACGTTGTGTTTTCAACTCTGCATACGGTTGGTGCTGCAAAGACCATAGATCGT
ATAATTGACGTGTTTCCTCCACATCAACAGCAGCAGATTAGAGTTCAGCTATCTACTGTTCTTCAGGGTGTTGTTTCACA
GCAACTTTTGACAAGACGCGATGGAAAAGGTCGAGTTGTTGCAACAGAGGTAATGATTTGTAATCCTGCTATAAGAAATC
TTATTAGAGAGAACAAGACTTATCAAATACCGTCCATAATTCAAACACATCAGCGGGTTGGTATGATAACTATGGAACAG
TCTTTGATAGAACTTTACAAACGAGGGCTTATTACAAGAGATGATGCATATGCTCATGCAACTGATTTCGACTATATGGA
AAAGTTATTGTCTGCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

97.159

0.503
  pilT Legionella pneumophila strain Lp02

51.471

96.591

0.497
  pilT Legionella pneumophila strain ERS1305867

51.471

96.591

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.87

94.034

0.497
  pilT Vibrio cholerae strain A1552

52.87

94.034

0.497
  pilT Acinetobacter baumannii strain A118

51.94

95.17

0.494
  pilT Acinetobacter nosocomialis M2

51.94

95.17

0.494
  pilT Acinetobacter baumannii D1279779

51.94

95.17

0.494
  pilT Neisseria meningitidis 8013

49.856

98.58

0.491
  pilT Acinetobacter baylyi ADP1

50.437

97.443

0.491
  pilT Pseudomonas stutzeri DSM 10701

50.437

97.443

0.491
  pilT Neisseria gonorrhoeae MS11

49.133

98.295

0.483
  pilT Pseudomonas aeruginosa PAK

49.706

96.591

0.48
  pilU Pseudomonas stutzeri DSM 10701

41.525

100

0.418
  pilU Acinetobacter baylyi ADP1

40.762

96.875

0.395
  pilU Vibrio cholerae strain A1552

39.181

97.159

0.381