Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OXH62_RS15365 Genome accession   NZ_CP113803
Coordinates   3331225..3332259 (-) Length   344 a.a.
NCBI ID   WP_007953348.1    Uniprot ID   J3FB13
Organism   Pseudomonas chlororaphis strain YJY915     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3326225..3337259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OXH62_RS15340 (OXH62_15280) - 3326699..3327970 (+) 1272 WP_375744275.1 dihydroorotase -
  OXH62_RS15345 (OXH62_15285) - 3328292..3329278 (+) 987 WP_375744277.1 lipid II-degrading bacteriocin -
  OXH62_RS15350 (OXH62_15290) - 3329318..3329722 (+) 405 WP_375744279.1 hypothetical protein -
  OXH62_RS15355 (OXH62_15295) - 3329797..3330231 (-) 435 WP_007953343.1 TM2 domain-containing protein -
  OXH62_RS15360 (OXH62_15300) - 3330481..3331098 (+) 618 WP_007953345.1 C40 family peptidase -
  OXH62_RS15365 (OXH62_15305) pilT 3331225..3332259 (-) 1035 WP_007953348.1 type IV pilus twitching motility protein PilT Machinery gene
  OXH62_RS15370 (OXH62_15310) - 3332316..3333002 (+) 687 WP_375744282.1 YggS family pyridoxal phosphate-dependent enzyme -
  OXH62_RS15375 (OXH62_15315) proC 3333026..3333844 (+) 819 WP_375744284.1 pyrroline-5-carboxylate reductase -
  OXH62_RS15380 (OXH62_15320) - 3333855..3334445 (+) 591 WP_007953355.1 YggT family protein -
  OXH62_RS15385 (OXH62_15325) - 3334442..3334729 (+) 288 WP_127800408.1 DUF167 domain-containing protein -
  OXH62_RS15390 (OXH62_15330) - 3334919..3336058 (+) 1140 WP_085605883.1 homoserine O-acetyltransferase -
  OXH62_RS15395 (OXH62_15335) metW 3336066..3336686 (+) 621 WP_007953362.1 methionine biosynthesis protein MetW -
  OXH62_RS15400 (OXH62_15340) - 3336713..3337147 (+) 435 WP_007953365.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38071.74 Da        Isoelectric Point: 7.0157

>NTDB_id=763147 OXH62_RS15365 WP_007953348.1 3331225..3332259(-) (pilT) [Pseudomonas chlororaphis strain YJY915]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=763147 OXH62_RS15365 WP_007953348.1 3331225..3332259(-) (pilT) [Pseudomonas chlororaphis strain YJY915]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTGGATGGCGACGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTCCCCGGCGTGGCGCGC
TTTCGGGTCAATGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTGTTCCGGACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGCCATGGGCGACGTCTTTCGCAAGATCACCGATGCGCCACGCGGGCTGGTGCTGGTCACCGGGCCGACCGGCT
CCGGCAAGTCCACGACCCTGGCGGCAATGATCGATTACCTCAACACCCATCGCCATCACCACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGTTTCGCCAC
CGCGTTGCGTTCAGCGCTGCGCGAAGACCCGGACGTGATCCTGGTGGGCGAGATGCGTGATCTGGAAACCATTCGCCTGG
CGCTGACCGCCGCCGAAACCGGGCACCTGGTGTTCGGCACCCTGCACACCACGTCGGCAGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCGGGGACGAAAAGTCCATGGTGCGCTCGATGCTCTCGGAGTCGTTGCTGGCGGTGGTGTCGCAGAC
GCTGATCAAGAAGATCGGCGGTGGTCGGGTGGCGGCGCACGAGATCATGCTGGGCACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATACAGACCGGCGGCTCGCTGGGGATGCAGACACTCGACATGTGCCTG
AAGGATCTGGTGACCAAAGGCTTGATCAGCCGCGAACATGCGCGGGAGAAGGCGCGCACGCCGGATAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J3FB13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398