Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7R64_RS04215 Genome accession   NZ_AP022020
Coordinates   869467..870450 (+) Length   327 a.a.
NCBI ID   WP_106925700.1    Uniprot ID   A0A2T2Y3H0
Organism   Klebsiella sp. WP3-S18-ESBL-05     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 864467..875450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R64_RS04185 (WP3S18E05_08300) - 864852..865838 (-) 987 WP_106925688.1 TRAP transporter substrate-binding protein -
  H7R64_RS04190 (WP3S18E05_08310) hemW 866143..867279 (-) 1137 WP_106925690.1 radical SAM family heme chaperone HemW -
  H7R64_RS04195 (WP3S18E05_08320) - 867272..867865 (-) 594 WP_106925692.1 XTP/dITP diphosphatase -
  H7R64_RS04200 (WP3S18E05_08330) yggU 867874..868164 (-) 291 WP_182945228.1 DUF167 family protein YggU -
  H7R64_RS04205 (WP3S18E05_08340) - 868161..868727 (-) 567 WP_106925696.1 YggT family protein -
  H7R64_RS04210 (WP3S18E05_08350) - 868746..869450 (-) 705 WP_106925698.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7R64_RS04215 (WP3S18E05_08360) pilT 869467..870450 (+) 984 WP_106925700.1 type IV pilus twitching motility protein PilT Machinery gene
  H7R64_RS04220 (WP3S18E05_08370) - 870981..871634 (+) 654 WP_106925702.1 LuxR C-terminal-related transcriptional regulator -
  H7R64_RS04225 (WP3S18E05_08380) ruvX 871693..872109 (-) 417 WP_064542651.1 Holliday junction resolvase RuvX -
  H7R64_RS04230 (WP3S18E05_08390) - 872109..872672 (-) 564 WP_106925704.1 YqgE/AlgH family protein -
  H7R64_RS04235 (WP3S18E05_08400) gshB 872819..873766 (-) 948 WP_106925706.1 glutathione synthase -
  H7R64_RS04240 (WP3S18E05_08410) rsmE 873779..874510 (-) 732 WP_106925708.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H7R64_RS04245 (WP3S18E05_08420) endA 874592..875299 (-) 708 WP_106925710.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 327 a.a.        Molecular weight: 35555.72 Da        Isoelectric Point: 6.7330

>NTDB_id=76261 H7R64_RS04215 WP_106925700.1 869467..870450(+) (pilT) [Klebsiella sp. WP3-S18-ESBL-05]
MDIQEIVTLSVKHNVSDLHLCSSGNARWRRAGKLEIAPFSAPHLGVLMDGWLSESQQQTWLRQRQLDFSLSLTGCPRLRA
SAFAHSGGVSLVLRILPTRCPTLDELHPPVALAEMLQEESGLILVTGATGSGKSTTLAAMVDYLNHHQAGHILTLEDPVE
FLHTSEGCLIHQREVGEHAASFADALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDV
FPAQEKEPVRNQLASSLCAVLAQKLQPDNRGGRVALFEMLINTPAVGNLIREGKTHQIAGVMQTGLQSGMQTFSQSYQQR
VKAGMLS

Nucleotide


Download         Length: 984 bp        

>NTDB_id=76261 H7R64_RS04215 WP_106925700.1 869467..870450(+) (pilT) [Klebsiella sp. WP3-S18-ESBL-05]
ATGGATATACAAGAAATCGTGACGCTTAGTGTAAAGCATAACGTGTCCGATCTACACCTATGTAGCAGTGGAAACGCGCG
CTGGCGTAGGGCGGGTAAGCTGGAAATAGCGCCGTTTAGCGCCCCGCATCTCGGCGTGCTAATGGACGGCTGGCTAAGTG
AAAGCCAGCAACAGACGTGGCTGCGGCAGCGACAGCTTGATTTTTCACTGTCGCTGACCGGCTGCCCGCGCCTGCGTGCC
AGTGCGTTTGCCCACAGCGGCGGTGTCTCGCTGGTGCTGCGCATACTGCCGACGCGCTGCCCTACGCTTGACGAACTGCA
TCCGCCGGTGGCGTTAGCGGAGATGCTACAGGAGGAGAGCGGGCTGATTCTGGTGACCGGGGCGACGGGCAGCGGAAAAT
CCACCACGCTGGCGGCGATGGTGGATTACCTTAATCATCATCAGGCCGGGCATATTTTGACGCTGGAAGACCCGGTTGAG
TTTCTGCATACCAGCGAGGGGTGTCTGATTCACCAGCGCGAGGTCGGTGAGCACGCCGCGTCTTTTGCCGATGCGCTGCG
AGTCGCACTACGTCAGGATCCGGATGTGATTCTGCTGGGTGAGCTGCGCGATAGCGAAACCATTCGTCTGGCGCTGACGG
CGGCGGAAACCGGGCACCTGGTGCTGGCAACGCTGCATACGCGCGGCGCGGCACAGGCCATAGAGCGGCTGGTGGACGTG
TTCCCCGCACAGGAAAAAGAGCCCGTGCGCAATCAGCTGGCCAGCAGCCTCTGCGCGGTGCTGGCGCAAAAGCTGCAGCC
GGACAATCGCGGCGGGCGCGTCGCGCTGTTTGAAATGCTGATTAACACGCCGGCGGTGGGTAATCTCATTCGCGAAGGGA
AAACGCACCAGATAGCAGGCGTGATGCAGACCGGGTTGCAAAGCGGCATGCAGACCTTCAGCCAAAGCTATCAACAGCGA
GTTAAGGCGGGGATGTTATCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T2Y3H0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.697

100

0.502
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.697

100

0.502
  pilT Neisseria meningitidis 8013

48.64

100

0.492
  pilT Neisseria gonorrhoeae MS11

48.338

100

0.489
  pilT Pseudomonas aeruginosa PAK

46.364

100

0.468
  pilT Pseudomonas stutzeri DSM 10701

46.061

100

0.465
  pilT Legionella pneumophila strain Lp02

46.061

100

0.465
  pilT Legionella pneumophila strain ERS1305867

46.061

100

0.465
  pilT Acinetobacter baumannii strain A118

45.758

100

0.462
  pilT Acinetobacter nosocomialis M2

45.758

100

0.462
  pilT Acinetobacter baumannii D1279779

45.758

100

0.462
  pilT Acinetobacter baylyi ADP1

45.758

100

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.634

96.942

0.462
  pilU Acinetobacter baylyi ADP1

40.248

98.777

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.312

97.859

0.394
  pilU Vibrio cholerae strain A1552

39.506

99.083

0.391

Multiple sequence alignment