Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OWC53_RS18150 Genome accession   NZ_CP113504
Coordinates   3957262..3958278 (-) Length   338 a.a.
NCBI ID   WP_268197750.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain 21PCA_AGRO2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3952262..3963278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OWC53_RS18135 (OWC53_18120) nsrR 3952485..3952910 (-) 426 WP_014916532.1 nitric oxide-sensing transcriptional repressor NsrR -
  OWC53_RS18140 (OWC53_18125) - 3953238..3954809 (+) 1572 WP_268197748.1 methyl-accepting chemotaxis protein -
  OWC53_RS18145 (OWC53_18130) - 3955461..3957293 (+) 1833 WP_268197749.1 diguanylate cyclase -
  OWC53_RS18150 (OWC53_18135) pilT 3957262..3958278 (-) 1017 WP_268197750.1 type IV pilus twitching motility protein PilT Machinery gene
  OWC53_RS18155 (OWC53_18140) - 3958299..3959012 (+) 714 WP_268197751.1 YggS family pyridoxal phosphate-dependent enzyme -
  OWC53_RS18160 (OWC53_18145) proC 3959107..3959928 (+) 822 WP_268198896.1 pyrroline-5-carboxylate reductase -
  OWC53_RS18165 (OWC53_18150) - 3959977..3960531 (+) 555 WP_010279107.1 YggT family protein -
  OWC53_RS18170 (OWC53_18155) yggU 3960528..3960818 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  OWC53_RS18175 (OWC53_18160) rdgB 3960836..3961429 (+) 594 WP_075278319.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  OWC53_RS18180 (OWC53_18165) hemW 3961422..3962564 (+) 1143 WP_075278320.1 radical SAM family heme chaperone HemW -
  OWC53_RS18185 (OWC53_18170) - 3962790..3963053 (+) 264 WP_225181991.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36707.92 Da        Isoelectric Point: 6.6533

>NTDB_id=762469 OWC53_RS18150 WP_268197750.1 3957262..3958278(-) (pilT) [Pectobacterium brasiliense strain 21PCA_AGRO2]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLATLQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=762469 OWC53_RS18150 WP_268197750.1 3957262..3958278(-) (pilT) [Pectobacterium brasiliense strain 21PCA_AGRO2]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCCGCTCAGAGAGAGCAACTGCGGCAGGCTGGGCAGGTGGACGGGGCATTAATGCTGCCCGATGGGCAA
CGCCTGCGGGTGAATGTATTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGTATTATCCCGTCCGTTCAACCTTCATT
GGCGACGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAGTCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTCATCCATGCCAGCCGGCGTTGCCTGATTCAGCAGCGGGAGATTGGTGCGCACAGCGCATCGTTTGC
GCAGGCGCTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTAGCTACCTGTTTGCAGGCCGTGGTGACGCA
AAGGCTGCTGCCCGCTGCGCAAGGTGGCCGAATTGCGCTTTATGAAGTGCTGACGGCGACGGCGGCTGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

97.633

0.503
  pilT Vibrio cholerae strain A1552

51.515

97.633

0.503
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.64

97.929

0.476
  pilT Acinetobacter baumannii strain A118

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

99.704

0.423
  pilU Vibrio cholerae strain A1552

39.067

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.64

98.521

0.391
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367