Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OT487_RS03665 Genome accession   NZ_CP113197
Coordinates   737087..738067 (+) Length   326 a.a.
NCBI ID   WP_002916609.1    Uniprot ID   W8UCM3
Organism   Klebsiella pneumoniae strain SCLC3-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 732087..743067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OT487_RS03635 (OT487_03635) - 732418..733464 (-) 1047 WP_064152132.1 sugar ABC transporter substrate-binding protein -
  OT487_RS03640 (OT487_03640) hemW 733757..734896 (-) 1140 WP_048993541.1 radical SAM family heme chaperone HemW -
  OT487_RS03645 (OT487_03645) - 734889..735482 (-) 594 WP_048993540.1 XTP/dITP diphosphatase -
  OT487_RS03650 (OT487_03650) yggU 735495..735785 (-) 291 WP_002916614.1 DUF167 family protein YggU -
  OT487_RS03655 (OT487_03655) - 735782..736348 (-) 567 WP_002916613.1 YggT family protein -
  OT487_RS03660 (OT487_03660) - 736369..737070 (-) 702 WP_002916611.1 YggS family pyridoxal phosphate-dependent enzyme -
  OT487_RS03665 (OT487_03665) pilT 737087..738067 (+) 981 WP_002916609.1 type IV pilus twitching motility protein PilT Machinery gene
  OT487_RS03670 (OT487_03670) - 738416..739069 (+) 654 WP_071571001.1 LuxR C-terminal-related transcriptional regulator -
  OT487_RS03675 (OT487_03675) ruvX 739122..739538 (-) 417 WP_002916605.1 Holliday junction resolvase RuvX -
  OT487_RS03680 (OT487_03680) - 739538..740101 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  OT487_RS03685 (OT487_03685) gshB 740276..741226 (-) 951 WP_002916600.1 glutathione synthase -
  OT487_RS03690 (OT487_03690) rsmE 741247..741978 (-) 732 WP_002916597.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OT487_RS03695 (OT487_03695) endA 742070..742777 (-) 708 WP_002916589.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35230.71 Da        Isoelectric Point: 6.8544

>NTDB_id=760847 OT487_RS03665 WP_002916609.1 737087..738067(+) (pilT) [Klebsiella pneumoniae strain SCLC3-1]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGRLQPAPFPAPDIANLLNDWLDAAQLLHWQEHGQIDFALTLACGARLRA
SAFAHTRGISLVLRLLPEQCPRLDTLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREVGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQC
VAAGAL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=760847 OT487_RS03665 WP_002916609.1 737087..738067(+) (pilT) [Klebsiella pneumoniae strain SCLC3-1]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCACG
CTGGCGGCGGCAGGGCAGGCTGCAGCCCGCGCCCTTTCCCGCGCCGGATATTGCGAATTTACTCAACGACTGGCTCGATG
CCGCACAGTTACTGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGACTCTGGCCTGCGGTGCGCGGCTGCGCGCC
AGCGCCTTCGCCCATACGCGTGGGATATCGCTGGTGCTGCGCCTCCTTCCTGAACAGTGCCCGCGTCTGGACACGCTCGG
CGCCCCGCCTGCGCTGAGCGAACTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCAATGGTGGGGCACCTCAATCAGCATCTTGATGGCCATATTCTGACGCTTGAAGATCCGGTGGAA
TTTATTCATCACAGCGAGCGCTGTCTGATCCAGCAGCGGGAAGTTGGCCGTCACTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGATAGCGAAACCATTCGCCTGGCGTTAACCG
CGGCGGAGACCGGGCACCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTAATCGATGTC
TTTCCGGCGGAGGAGAAAGATCAGGTTCGTAGCCAGCTTGCCGGAAGCCTGTGCGCGGTACTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTCTGGTCAACACGCCAGCGGTGGCGAATTTGATCCGTGAAGGAA
AAGTACATCAATTGCCTGGTGTGATGCAGACCGGGATGCAGGCCGGAATGTTGACCTTTACCCAGAGTTTTCAGCAATGT
GTCGCTGCAGGCGCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8UCM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

100

0.518
  pilT Vibrio cholerae strain A1552

51.368

100

0.518
  pilT Acinetobacter baylyi ADP1

48.936

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

48.024

100

0.485
  pilT Acinetobacter baumannii strain A118

48.024

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

47.72

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.148

99.387

0.479
  pilT Legionella pneumophila strain Lp02

48.148

99.387

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

97.239

0.436
  pilU Vibrio cholerae strain A1552

41.358

99.387

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.875

98.466

0.393