Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKJ05_RS21520 Genome accession   NZ_CP113163
Coordinates   4504412..4505392 (+) Length   326 a.a.
NCBI ID   WP_267588310.1    Uniprot ID   -
Organism   Citrobacter braakii strain LBA3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4499412..4510392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKJ05_RS21490 (MKJ05_21490) - 4499788..4500774 (-) 987 WP_267588308.1 TRAP transporter substrate-binding protein -
  MKJ05_RS21495 (MKJ05_21495) hemW 4501088..4502224 (-) 1137 WP_267588309.1 radical SAM family heme chaperone HemW -
  MKJ05_RS21500 (MKJ05_21500) - 4502217..4502810 (-) 594 WP_016154402.1 XTP/dITP diphosphatase -
  MKJ05_RS21505 (MKJ05_21505) yggU 4502818..4503108 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  MKJ05_RS21510 (MKJ05_21510) - 4503105..4503671 (-) 567 WP_016154401.1 YggT family protein -
  MKJ05_RS21515 (MKJ05_21515) - 4503690..4504394 (-) 705 WP_199146396.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKJ05_RS21520 (MKJ05_21520) pilT 4504412..4505392 (+) 981 WP_267588310.1 type IV pilus twitching motility protein PilT Machinery gene
  MKJ05_RS21525 (MKJ05_21525) ruvX 4505389..4505805 (-) 417 WP_267588311.1 Holliday junction resolvase RuvX -
  MKJ05_RS21530 (MKJ05_21530) - 4505805..4506368 (-) 564 WP_016154395.1 YqgE/AlgH family protein -
  MKJ05_RS21535 (MKJ05_21535) gshB 4506545..4507492 (-) 948 WP_114681110.1 glutathione synthase -
  MKJ05_RS21540 (MKJ05_21540) rsmE 4507505..4508236 (-) 732 WP_267588312.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MKJ05_RS21545 (MKJ05_21545) endA 4508312..4509019 (-) 708 WP_267588313.1 deoxyribonuclease I -
  MKJ05_RS21550 (MKJ05_21550) - 4509114..4509611 (-) 498 WP_199146389.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35937.01 Da        Isoelectric Point: 6.1703

>NTDB_id=760698 MKJ05_RS21520 WP_267588310.1 4504412..4505392(+) (pilT) [Citrobacter braakii strain LBA3]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVETAPFTPPDVENLLMCWLSEQQQVQCREQGQIDFALTLANSWRLRA
SAFAHQQGTSLALRLLPLECPCLDDLQTPEAIPELLHSENGLVLVTGATGSGKSTTLAAMVEYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAITRLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=760698 MKJ05_RS21520 WP_267588310.1 4504412..4505392(+) (pilT) [Citrobacter braakii strain LBA3]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAACCGCACCATTTACTCCCCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGCCGGGAACAGGGGCAGATTGATTTTGCCCTTACGTTAGCGAACTCCTGGCGTCTGCGCGCC
AGCGCATTTGCTCATCAGCAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTTGTTTAGACGATCTCCA
GACTCCCGAGGCCATACCTGAACTGCTGCACAGTGAAAATGGATTAGTTCTGGTTACTGGCGCCACCGGAAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATGTTGCGGGGCATATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGTTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCGCAAGCTATCACGCGACTGGTGGACACC
TTTCCCGCACAGGAAAAAGATCCGGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAGCTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Legionella pneumophila strain Lp02

47.799

97.546

0.466
  pilT Legionella pneumophila strain ERS1305867

47.799

97.546

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.956

97.239

0.408
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365