Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OVN20_RS04445 Genome accession   NZ_CP113090
Coordinates   941724..942767 (+) Length   347 a.a.
NCBI ID   WP_324287741.1    Uniprot ID   -
Organism   Microcella daejeonensis strain MMS21-STM26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 936724..947767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OVN20_RS04420 (OVN20_04420) - 936972..937448 (+) 477 WP_267738816.1 hypothetical protein -
  OVN20_RS04425 (OVN20_04425) - 937445..937942 (+) 498 WP_267738817.1 hypothetical protein -
  OVN20_RS04430 (OVN20_04430) - 937989..938879 (+) 891 WP_267738818.1 hypothetical protein -
  OVN20_RS04435 (OVN20_04435) - 939034..940707 (+) 1674 WP_267738819.1 GspE/PulE family protein -
  OVN20_RS04440 (OVN20_04440) - 940852..941214 (-) 363 WP_267738821.1 hypothetical protein -
  OVN20_RS04445 (OVN20_04445) pilT 941724..942767 (+) 1044 WP_324287741.1 type IV pilus twitching motility protein PilT Machinery gene
  OVN20_RS04450 (OVN20_04450) - 942769..944007 (+) 1239 WP_267738825.1 type II secretion system F family protein -
  OVN20_RS13450 - 944249..944674 (+) 426 WP_324287742.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OVN20_RS04460 (OVN20_04460) - 944808..946184 (+) 1377 WP_267738827.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OVN20_RS04465 (OVN20_04465) - 946181..946882 (+) 702 WP_267738828.1 PilW family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37524.67 Da        Isoelectric Point: 6.6687

>NTDB_id=760128 OVN20_RS04445 WP_324287741.1 941724..942767(+) (pilT) [Microcella daejeonensis strain MMS21-STM26]
MNAPPRIRLHGALEDLPSTVPWTPEKMEGALHSIMTPAQRDTFEEHLELDFAFTLSAESRFRVNVYQQRGNLGAAFRLIP
TEIKPLANLGVPASVSRFATLARGLVLVTGPTGSGKSTTLAGLIDLVNRTRTDHIVTVEDPIEFMHENKKSLINQREVGS
DTHSFANALKHVLRQDPDVILIGELRDLETISVALTAAETGHLVFATLHTQSAGQTIDRVIDVFPPHQQGQVRAQLASTL
QGVVCQTLIPRASGGGRAVATEILVANAAIGNLIREGKTFQIPSALQAGRELGMHTLDQHLADLVNHGVITHQAAMEKAQ
DTENLRQLIHRAESNGQMAGAGMNGSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=760128 OVN20_RS04445 WP_324287741.1 941724..942767(+) (pilT) [Microcella daejeonensis strain MMS21-STM26]
GTGAACGCGCCTCCGCGCATCCGCCTGCACGGGGCCCTGGAGGATCTGCCCTCGACGGTCCCGTGGACGCCCGAGAAGAT
GGAGGGAGCGCTGCACAGCATCATGACGCCCGCCCAGCGCGACACCTTCGAGGAGCACCTCGAGCTCGACTTCGCATTCA
CGCTCTCGGCAGAGAGCCGGTTCCGCGTGAACGTCTATCAGCAGCGCGGCAATCTCGGCGCGGCGTTCCGTCTCATCCCG
ACCGAGATCAAGCCGCTCGCCAACCTCGGTGTGCCGGCCTCGGTCTCCCGCTTCGCCACCCTCGCCCGCGGCCTCGTGCT
GGTGACCGGCCCGACCGGTTCCGGCAAGTCGACCACGCTCGCCGGACTCATCGACCTGGTGAACCGCACCCGCACCGATC
ACATCGTGACCGTCGAGGACCCGATCGAGTTCATGCACGAGAACAAGAAGTCGCTCATCAACCAGCGCGAGGTCGGCAGC
GACACCCACTCCTTCGCCAACGCACTCAAGCACGTGCTGCGGCAGGACCCGGACGTCATCCTCATCGGCGAGCTCCGTGA
CCTCGAGACCATCTCCGTCGCGCTGACCGCGGCCGAGACCGGGCACCTCGTGTTCGCGACCCTGCACACGCAGAGCGCCG
GTCAGACGATCGACCGCGTCATCGACGTGTTCCCGCCGCACCAGCAGGGCCAGGTGCGGGCGCAGCTCGCCTCGACCCTG
CAGGGCGTCGTGTGTCAGACGCTCATCCCTCGGGCCTCGGGCGGCGGCCGTGCGGTCGCGACCGAGATCCTCGTGGCCAA
CGCGGCCATCGGCAACCTCATCCGCGAGGGCAAGACGTTCCAGATCCCCTCGGCGTTGCAGGCGGGACGTGAACTCGGCA
TGCACACGCTCGATCAGCATCTCGCCGACCTCGTCAACCACGGGGTCATCACCCACCAGGCCGCGATGGAGAAGGCGCAG
GACACCGAGAACCTGCGTCAGCTCATCCACCGCGCCGAGTCGAACGGCCAGATGGCCGGCGCCGGCATGAACGGGAGCTT
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(32-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.235

97.983

0.522
  pilT Pseudomonas aeruginosa PAK

52.469

93.372

0.49
  pilT Acinetobacter nosocomialis M2

52.322

93.084

0.487
  pilT Acinetobacter baumannii D1279779

52.322

93.084

0.487
  pilT Acinetobacter baumannii strain A118

52.322

93.084

0.487
  pilT Legionella pneumophila strain Lp02

52.174

92.795

0.484
  pilT Legionella pneumophila strain ERS1305867

52.174

92.795

0.484
  pilT Pseudomonas stutzeri DSM 10701

51.703

93.084

0.481
  pilT Acinetobacter baylyi ADP1

51.543

93.372

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.201

91.643

0.478
  pilT Vibrio cholerae strain A1552

52.201

91.643

0.478
  pilT Neisseria gonorrhoeae MS11

49.845

93.084

0.464
  pilT Neisseria meningitidis 8013

49.536

93.084

0.461
  pilU Acinetobacter baylyi ADP1

40.597

96.542

0.392
  pilU Pseudomonas stutzeri DSM 10701

40.476

96.83

0.392
  pilU Vibrio cholerae strain A1552

41.401

90.49

0.375