Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OR613_RS03800 Genome accession   NZ_CP112887
Coordinates   774791..775771 (+) Length   326 a.a.
NCBI ID   WP_131048584.1    Uniprot ID   A0AAJ5QUA3
Organism   Klebsiella electrica strain S2-IND-01-C     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 769791..780771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OR613_RS03770 (OR613_03765) - 770102..771148 (-) 1047 WP_131048581.1 sugar ABC transporter substrate-binding protein -
  OR613_RS03775 (OR613_03770) hemW 771461..772600 (-) 1140 WP_131048582.1 radical SAM family heme chaperone HemW -
  OR613_RS03780 (OR613_03775) - 772593..773186 (-) 594 WP_100683436.1 XTP/dITP diphosphatase -
  OR613_RS03785 (OR613_03780) yggU 773199..773489 (-) 291 WP_032690761.1 DUF167 family protein YggU -
  OR613_RS03790 (OR613_03785) - 773486..774052 (-) 567 WP_100683434.1 YggT family protein -
  OR613_RS03795 (OR613_03790) - 774070..774774 (-) 705 WP_131048583.1 YggS family pyridoxal phosphate-dependent enzyme -
  OR613_RS03800 (OR613_03795) pilT 774791..775771 (+) 981 WP_131048584.1 type IV pilus twitching motility protein PilT Machinery gene
  OR613_RS03805 (OR613_03800) - 776107..776760 (+) 654 WP_131048585.1 LuxR C-terminal-related transcriptional regulator -
  OR613_RS03810 (OR613_03805) ruvX 776831..777247 (-) 417 WP_004867490.1 Holliday junction resolvase RuvX -
  OR613_RS03815 (OR613_03810) - 777247..777810 (-) 564 WP_100683430.1 YqgE/AlgH family protein -
  OR613_RS03820 (OR613_03815) gshB 778110..779057 (-) 948 WP_131048586.1 glutathione synthase -
  OR613_RS03825 (OR613_03820) rsmE 779078..779809 (-) 732 WP_100683428.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OR613_RS03830 (OR613_03825) endA 779898..780605 (-) 708 WP_271207409.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35579.88 Da        Isoelectric Point: 6.8607

>NTDB_id=758657 OR613_RS03800 WP_131048584.1 774791..775771(+) (pilT) [Klebsiella electrica strain S2-IND-01-C]
MELEEIVALSVKHNVSDLHLCNTSAARWRRQGKLEPAPFHTPDIVKWLDTWLNEHQRAHWLANGQVDFALAPVGVPRLRA
NAFSHAQGISLALRLLPESCPRIEALHVPAALTELLNEESGLILVTGATGSGKSTTLAAMVNHLNQQREGHILTLEDPVE
FVHHSERCLIQQREIGRDCPSFSSALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAIERLVDV
FPAQEKEPIRSQLAGSLCAVLAQKLLPTASGGRVALYELLLNTLAVANLIREGKTHQLPGVMQTGQQAGMLTFTQSFQQR
VAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=758657 OR613_RS03800 WP_131048584.1 774791..775771(+) (pilT) [Klebsiella electrica strain S2-IND-01-C]
ATGGAGCTGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATACCTCGGCAGCGCG
CTGGCGTCGGCAGGGAAAGCTTGAGCCGGCACCATTTCACACGCCGGATATCGTGAAATGGCTTGATACGTGGCTCAATG
AGCATCAGCGCGCGCACTGGCTGGCCAACGGACAGGTCGATTTTGCCTTAGCTCCCGTCGGCGTACCGCGCCTTCGCGCC
AACGCATTTTCTCACGCCCAGGGGATTTCGTTAGCGCTGCGCCTGTTGCCGGAAAGCTGCCCCCGGATTGAGGCGCTGCA
TGTGCCCGCGGCGCTGACGGAACTGCTGAATGAAGAAAGCGGTTTGATTCTGGTAACCGGCGCCACCGGCAGCGGTAAGT
CGACCACGCTGGCGGCGATGGTCAATCATCTCAACCAGCAGCGTGAAGGGCATATTCTGACCCTTGAAGATCCGGTGGAG
TTTGTCCATCACAGCGAGCGATGTCTGATTCAGCAGCGTGAAATCGGGCGCGACTGCCCCTCTTTTTCCTCGGCGCTGCG
GGCGGCGCTGCGCCAGGATCCCGATGTGATTCTGCTGGGTGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACCG
CTGCCGAGACCGGACATCTGGTGATGGCCACGCTGCATACCCGAGGTGCGGCTCAGGCGATTGAGCGTCTGGTGGATGTC
TTCCCCGCGCAGGAAAAAGAGCCCATACGTAGCCAGCTAGCGGGAAGTTTATGTGCGGTGCTGGCACAAAAATTACTGCC
GACAGCGTCAGGGGGAAGAGTTGCGCTATACGAGCTTTTGCTGAACACCCTGGCGGTAGCGAATTTGATCCGCGAAGGGA
AAACTCATCAACTGCCGGGGGTGATGCAAACCGGGCAACAAGCCGGCATGCTGACTTTTACGCAAAGTTTTCAACAGCGT
GTGGCAGCGGGTCTGTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.456

100

0.509
  pilT Vibrio cholerae strain A1552

50.456

100

0.509
  pilT Neisseria meningitidis 8013

47.576

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.273

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

46.667

100

0.472
  pilT Acinetobacter baumannii strain A118

46.364

100

0.469
  pilT Acinetobacter baumannii D1279779

46.364

100

0.469
  pilT Acinetobacter nosocomialis M2

46.061

100

0.466
  pilT Legionella pneumophila strain ERS1305867

46.201

100

0.466
  pilT Legionella pneumophila strain Lp02

46.201

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.897

100

0.463
  pilT Acinetobacter baylyi ADP1

45.455

100

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.057

97.239

0.448
  pilU Pseudomonas stutzeri DSM 10701

39.879

100

0.405
  pilU Vibrio cholerae strain A1552

40.123

99.387

0.399
  pilU Acinetobacter baylyi ADP1

38.006

98.466

0.374