Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   OA858_RS15940 Genome accession   NZ_CP112874
Coordinates   3500274..3502286 (-) Length   670 a.a.
NCBI ID   WP_281006185.1    Uniprot ID   -
Organism   Pseudanabaena galeata CCNP1313     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3495274..3507286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OA858_RS15920 (OA858_15915) - 3495960..3496424 (-) 465 WP_281006181.1 pentapeptide repeat-containing protein -
  OA858_RS15925 (OA858_15920) miaA 3496829..3497743 (+) 915 WP_281006182.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  OA858_RS15930 (OA858_15925) - 3497774..3498982 (-) 1209 WP_281006184.1 type II secretion system F family protein -
  OA858_RS15935 (OA858_15930) - 3499027..3500181 (-) 1155 WP_281009426.1 type IV pilus twitching motility protein PilT -
  OA858_RS15940 (OA858_15935) pilB/pilB1 3500274..3502286 (-) 2013 WP_281006185.1 GspE/PulE family protein Machinery gene
  OA858_RS15945 (OA858_15940) - 3502560..3504659 (-) 2100 WP_281006186.1 D-alanyl-lipoteichoic acid biosynthesis protein DltD -
  OA858_RS15950 (OA858_15945) - 3504769..3505146 (-) 378 WP_281006187.1 metalloregulator ArsR/SmtB family transcription factor -
  OA858_RS15955 (OA858_15950) - 3505244..3505411 (+) 168 WP_190580297.1 metallothionein -
  OA858_RS15960 (OA858_15955) - 3505623..3506450 (+) 828 WP_281006188.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  OA858_RS15965 (OA858_15960) - 3506554..3506883 (+) 330 WP_281006189.1 DUF4359 domain-containing protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74580.18 Da        Isoelectric Point: 5.4412

>NTDB_id=758632 OA858_RS15940 WP_281006185.1 3500274..3502286(-) (pilB/pilB1) [Pseudanabaena galeata CCNP1313]
MNTSDQAPTTAASRNKTKALAKRGGRTPFESKLIQAGHATSDQFDRAVKDSQEQNVPFLSVLEQILGQQLAPDITRYYKR
QQLFELRVLYGLEPIDPDVEIDKFDIPTLSTLLDSNIIPISTCRDCEVLPLVKTENSITIAVVSPDSYKVQNEIARLLKN
ITIVRRVISREDFHRTFDNIVQFQVNKSAKAEGAISDEDLQIKDDVFSEDITGADDGDELMVGGENSAPIISLVDKILVK
ALKEGCSDIHIEPQEKDLCIRLRKDGVLREYFFLSEGKRLPRNIVNAVISRFKIISNLNIAEKRVPQDGKLKRNFQGRRV
DFRVNTCPTQNGEKVVLRILDNSNTQLGLNKLISDPESLELMQSFAKRPYGLILVTGPTGSGKTTTLYSTLAECNDPGIN
ISTAEDPVEYNLPGLTQCQVLREKGMTFASILRAFLRQDPDVILVGETRDAETAKTAIEAALTGHLVLTTLHTNDAPSSI
ARLEEMGVEPFMASTAIIGVLAQRLLRRICDNCRIPYNPSQEELDRFGLPSSDLEKTFYRANIVNREAMLPGQRVCPKCS
GSGYKGRAGVYEIMKMTERLQSAINKGATTEVIKEIAVQEGMKTLMAYAFDLVRNGATTLDEVLRVVYTDKGREAEERAR
RGKGLECDNCDAMLTPETVECPYCTTPRTF

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=758632 OA858_RS15940 WP_281006185.1 3500274..3502286(-) (pilB/pilB1) [Pseudanabaena galeata CCNP1313]
ATGAATACATCAGATCAAGCGCCAACCACTGCCGCAAGCAGAAACAAAACTAAAGCTCTGGCAAAACGGGGAGGTCGAAC
TCCCTTCGAGAGCAAACTGATTCAAGCAGGACATGCCACCTCAGACCAGTTCGATCGCGCTGTCAAAGATAGCCAAGAAC
AAAATGTTCCATTCCTCAGCGTCCTAGAGCAAATTCTGGGGCAGCAACTAGCTCCAGACATTACCCGCTATTACAAGCGC
CAACAACTTTTTGAACTGCGGGTTCTTTACGGGCTAGAACCGATCGATCCCGATGTCGAAATTGACAAGTTTGACATTCC
TACTCTCAGCACCTTACTTGACTCCAACATTATTCCAATTTCCACTTGTCGGGACTGCGAAGTATTGCCGCTAGTTAAGA
CTGAAAATAGCATTACGATCGCTGTAGTCTCTCCCGACAGCTACAAAGTCCAAAATGAGATTGCTCGCCTACTTAAAAAC
ATCACAATCGTTCGGCGCGTGATCTCCCGTGAAGATTTTCATCGCACCTTTGACAATATTGTCCAATTTCAAGTTAACAA
GAGTGCTAAGGCTGAAGGAGCAATCAGCGACGAAGATCTTCAAATCAAAGATGATGTTTTTAGTGAAGATATCACTGGGG
CAGATGATGGCGATGAACTCATGGTGGGCGGCGAAAATTCCGCTCCAATCATCAGTCTCGTCGATAAAATTCTCGTTAAA
GCGCTCAAAGAAGGATGTTCAGATATCCACATCGAACCACAAGAAAAAGACCTTTGCATCCGTCTTCGTAAAGATGGAGT
ACTGCGCGAATACTTCTTCCTGAGTGAAGGCAAGCGCTTACCACGTAACATCGTCAATGCTGTTATCTCGCGCTTTAAGA
TTATCTCCAACCTGAATATTGCTGAAAAGCGCGTTCCTCAAGATGGCAAACTGAAGCGCAACTTCCAAGGTCGGCGGGTG
GATTTCCGCGTCAATACCTGTCCCACTCAAAATGGGGAAAAAGTAGTTTTGCGGATTCTGGATAACTCCAATACGCAACT
TGGACTTAATAAACTGATTAGCGATCCCGAAAGCTTAGAACTGATGCAAAGCTTTGCCAAACGTCCCTATGGATTGATCT
TGGTTACAGGTCCCACAGGTTCAGGCAAGACTACCACCCTATACTCGACCCTTGCTGAATGTAATGATCCTGGTATCAAC
ATCAGTACTGCTGAAGATCCTGTTGAATATAACTTACCTGGTCTGACCCAGTGCCAAGTGCTGCGTGAAAAGGGAATGAC
ATTTGCGAGCATTCTCCGTGCCTTCTTACGACAAGATCCTGACGTAATCCTTGTTGGTGAAACCAGAGATGCAGAAACAG
CAAAAACAGCGATCGAAGCTGCGCTAACAGGGCACTTAGTTCTCACGACATTACACACCAACGACGCGCCTAGCAGTATT
GCTCGTCTGGAAGAAATGGGCGTAGAGCCATTTATGGCAAGTACCGCGATCATTGGTGTTCTTGCCCAACGTCTCCTTCG
CCGAATCTGTGACAACTGCCGCATCCCCTATAACCCTAGCCAAGAAGAACTCGATCGCTTCGGTTTACCCAGCAGCGATC
TCGAAAAAACCTTCTATCGAGCCAACATCGTTAACCGCGAAGCCATGCTACCTGGACAACGAGTATGTCCAAAATGTAGT
GGGTCTGGGTATAAAGGTCGAGCAGGTGTCTATGAGATCATGAAAATGACCGAACGCTTGCAAAGTGCCATCAACAAAGG
CGCAACAACCGAAGTAATTAAAGAAATTGCTGTCCAAGAGGGCATGAAGACTCTGATGGCATATGCTTTTGACTTGGTGC
GAAATGGAGCAACGACTCTAGACGAAGTGCTGCGAGTCGTATATACAGACAAAGGACGTGAAGCAGAAGAACGCGCCCGA
CGCGGTAAAGGGCTGGAATGTGACAACTGTGATGCTATGCTCACACCCGAAACAGTTGAATGCCCGTACTGTACAACGCC
TCGAACTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-508)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(79-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

52.096

99.701

0.519